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The effect of maternal nutrition on foetal serum potency in cell culture

Published online by Cambridge University Press:  02 September 2010

M. F. Thomas ,
M. H. Oliver  and
S. C. Hodgkinson
M. F. Thomas
Affiliation:
AgResearch, Ruakura Agricultural Research Centre, Private Bag 3123, Hamilton, New Zealand
M. H. Oliver
Affiliation:
AgResearch, Ruakura Agricultural Research Centre, Private Bag 3123, Hamilton, New Zealand
S. C. Hodgkinson
Affiliation:
AgResearch, Ruakura Agricultural Research Centre, Private Bag 3123, Hamilton, New Zealand
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Abstract

The influence of pre-slaughter nutrition on the potency of foetal serum in cell culture was studied. Ewes carrying late-gestation foetuses (120-day gestation) were either fasted for 66 h (F), fasted for 66 h but drenched with Ketol, a propylene glycol preparation, (5 × 120 ml doses; FK), given food ad libitum (A), or given food ad libitum and drenched with Ketol (5 × 120 ml doses; AK). Following slaughter foetal blood was collected for the determination of potency in cell culture using industry-standard cell culture bioassays: cloning efficiency, plating efficiency and a 96 h cell proliferation assay. Foetal serum insulin-like growth factor (IGF) concentrations were also measured. Pre-slaughter fasting or drenching with Ketol had no effect on the potency of foetal serum in any of the cell culture bioassays. Fasting significantly lowered foetal plasma IGF-1 levels (F < 0·01). Foetal IGF-2 levels were unaffected by fasting or drenching with Ketol.

Keywords

cell culturefoetal serumnutritionsomatomedin
Type
Research Article
Information
Animal Science , Volume 64 , Issue 1 , February 1997 , pp. 111 - 117
Copyright
Copyright © British Society of Animal Science 1997

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