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Effect of fermented liquid food and zinc bacitracin on microbial metabolism in the gut and sensoric profile of m. longissimus dorsi from entire male and female pigs

Published online by Cambridge University Press:  18 August 2016

L. L. Hansen ,
L. L. Mikkelsen ,
H. Agerhem ,
A. Laue ,
M. T. Jensen  and
B. B. Jensen
L. L. Hansen
Affiliation:
Department of Animal Product Quality, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
L. L. Mikkelsen
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
H. Agerhem
Affiliation:
Swedish Meats Research and Development AB, Kaevlinge, PO Box 504, SE-244 24 Kaevlinge, Sweden
A. Laue*
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
M. T. Jensen
Affiliation:
Department of Animal Product Quality, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
B. B. Jensen
Affiliation:
Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum, PO Box 50, DK-8830 Tjele, Denmark
*
†Present address: Danish Cooperative Farm Supply, Vesterbrogade 4A, DK-1504 Copenhagen V, Denmark
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Abstract

The aim of this work in pigs was to evaluate the effect of fermented liquid food (FLF) and the combination of FLF plus short-term addition of an antibiotic food additive zinc bacitracin (FLF + ZB) compared with fed non-pelleted dry food (NPDF), on microbial metabolism in the gut and the effects on flavour and odour attributes and profiles of pig meat as well as boar odour from entire male and female pigs.

At an average start weight of 60 kg, 108 pigs (54 males and 54 females) were equally allocated to three treatments according to pen-replicate, litter and sex. Microbial metabolism in the gut was studied in 24 pigs, eight from each treatment. For sensory profile evaluation (flavour, odour and tenderness) of pork loins from m. longissimus dorsi, 48 pigs (24 entire males and 24 females) were selected. The sensory evaluation of different qualities of flavour and odour as well as tenderness of cooked pork loins (LD) was done by a boar taint (skatole and androstenone) trained taste panel. To evaluate the odour and the flavour, a sensory profile analysis was performed which involved the following attributes: total off-odour/off-flavour, pig, urine, manure, naphthalene, rancid, sweet and sweat in the whole sample including both meat and fat.

Giving FLF to pigs significantly changed the microbiota in the gastro-intestinal tract compared with NPDF. In particular, the density of coliform bacteria was reduced in the gastro-intestinal tract of the pigs on FLF. Giving FLF demonstrated no effects on skatole concentration in caecum, colon, blood and backfat and boar odour attributes, whereas administration of FLF + ZB decreased the skatole concentrations and the typical boar odours, pig and manure odour, compared especially with the pigs on NPDF. However, meat from pigs given FLF either with or without zinc bacitracin had smaller but significantly worsened scores for three flavour attributes — pig flavour, rancid flavour and total off-flavour — compared with meat from NPDF pigs and the three flavour attributes were equally affected in both sexes. This seems to point to a significant worsening of meat flavour in pigs given FLF that is independent of sex and boar odour problems (skatole and androstenone).

Keywords

boar taintflavourliquid dietpig meatzinc bacitracin
Type
Growth, development and meat science
Information
Animal Science , Volume 71 , Issue 1 , August 2000 , pp. 65 - 80
Copyright
Copyright © British Society of Animal Science 2000

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References

Agergaard, N., Jensen, B. B., Laue, A. and Jensen, M. T. 1998. Production and absorption of skatole to portal vein blood following tryptophan infusion to hindgut. In Skatole and boar taint (ed. Klinth Jensen, W.), pp. 97–110. Danish Meat Research Institute, Roskilde, Denmark.Google Scholar
Agerhem, H. and Dijksterhuis, G. 1997. Description of the methodology used for the sensory assessment of boar taint in EU project AIR 3 PL942482. Proceedings of the 43rd international congress of meat science and technology, August 1997, New Zealand.Google Scholar
Agerhem, H. and Tornberg, E. 1994. A comparison of the off-flavour of shoulder butts from entire male and female pigs using expert and consumer panels. Proceedings of the 40th international congress of meat science and technology, 28 August-2 September, 1994, The Hague, The Netherlands.Google Scholar
Agerhem, H. and Tornberg, E. 1995. A comparison of the off-flavour of meat from entire male pigs cooked to two different internal end-point temperatures. Annual Meeting of the European Association for Animal Production, 27-29 September, 1995. Google Scholar
Dahl, J. 1997. [Feed-related risk factors for sub-clinical salmonella infections.] Veterinærinformation 6: 17–20.Google Scholar
Geary, T. M., Brooks, P. H., Morgan, D. T., Campbell, A. and Russel, P. J. 1996. Performance of weaner pigs fed ad libitum with liquid feed at different dry matter concentrations. Journal of the Science of Food and Agriculture 72: 17–24.3.0.CO;2-3>CrossRefGoogle Scholar
Hansen, L. L., Larsen, A. E., Jensen, B. B. and Hansen-Møller, J. 1997. Short time effect of zinc bacitracin and heavy fouling with faeces plus urine on boar taint. Animal Science 64: 351–363.CrossRefGoogle Scholar
Harris, N. D., Strong, D. H. and Sunde, M. L. 1968. Intestinal flora and chicken flavour. Journal of Food Science 33: 543.CrossRefGoogle Scholar
Hawe, S. M. and Walker, N. 1991. The effects of involuntary coprophagy on the production of skatole in growing pigs. Animal Production 53: 105–109.Google Scholar
Holdeman, L. V., Cato, E. P. and Moore, E. C. 1977. Anaerobic laboratory manual, fourth edition. Blacksburg Virginia Polytechnic Institute and State University.Google Scholar
Jensen, B. B., Agergaard, N., Hansen, L. L., Mikkelsen, L. L., Jensen, M. T. and. Laue, A. 1998. Effect of liquid feed on microbial production of skatole in the hind gut, skatole absorption to portal blood and skatole deposition in backfat. In Skatole and boar taint (ed. Klinth Jensen, W.), pp. 165–178. Danish Meat Research Institute, Roskilde, Denmark.Google Scholar
Jensen, B. B. and Jensen, M. T. 1998. Microbial production of skatole in the digestive tract of entire male pigs. In Skatole and boar taint (ed. Klinth Jensen, W.), pp. 41–75. Danish Meat Research Institute, Roskilde, Denmark.Google Scholar
Jensen, B. B. and Jørgensen, H. 1994. Effect of dietary fiber on microbial activity and microbial gas production in various regions of the gastrointestinal tract of pigs. Applied and Environmental Microbiology 60: 1897–1904.Google Scholar
Jensen, B. B. and Mikkelsen, L. L. 1998. Feeding liquid diets to pigs. Recent Advances in Animal Nutrition 1998, pp. 107–126. Butterworths, London.Google Scholar
Jensen, M. T., Cox, R. P. and Jensen, B. B. 1995. Microbial production of skatole in the hindgut of pigs given different diets and its relation to skatole deposition in backfat. Animal Science 61: 293–304.CrossRefGoogle Scholar
Kjeldsen, N. 1993. Practical experience with production and slaughter of entire male pigs. In Measurement and prevention of boar taint in entire male pigs (ed. Bonneau, M.), pp. 137–144. Institut National de la Recherche Agronomique, Paris.Google Scholar
Madsen, A., Østerballe, R., Mortensen, H. P., Bejerholm, C. and Barton, P. 1990a. [Meat quality. The influence of feeds on meat quality of growing pigs. 1.] Statens Husdyrbrugsforsøg (NIAS). 673. Beretning.Google Scholar
Madsen, A., Petersen, J. and Søgaard, A. 1990b. [Anatomic content of the female and castrated male pig fed according to scale or ad libitum and slaughtered at 20, 50, 80 or 110 kg liveweight.] Statens Husdyrbrugsforsøg (NIAS). 769. Meddelelse.Google Scholar
Mead, G. C. 1983. Significance of the intestinal microflora in relation to meat quality in poultry. In Quality of poultry meat (ed. Lahellec, C., Richard, F. H. and Colin, P.), pp. 107–122. WPSA, Federation of European Branches, Working Group no. 5, Ploufragan, France.Google Scholar
Mikkelsen, L. L. and Jensen, B. B. 1997. Effect of fermented liquid feed (FLF) on growth performance and microbial activity in the gastrointestinal tract of weaned piglets. In Digestive physiology in pigs. Proceedings of the seventh symposium on digestive physiology in pigs, 1997, Saint-Malo, France. European Association for Animal Production publication no. 88, pp. 639–642.Google Scholar
Mikkelsen, L. L., McCartney, A. L. and Jensen, B. B. 1999. Comparison of three selective media for isolation and enumeration of Bifidobacteria in piglet faecal and intestinal samples. In Proceedings of a conference on host microflora interface in health and disease, 20-21. May, Amsterdam, The Netherlands, p. MO8 (abstr.). Yakult, The Netherlands.Google Scholar
Miller, T. L. and Wolin, M. J. 1974. A serum bottle modification of the Hungate technique for cultivating obligate anaerobic bacteria. Applied Microbiology 27: 985–987.Google Scholar
Mortensen, A. B. and Sørensen, S. E. 1984. Relationship between boar taint and skatole determined with a new analysis method. Proceedings of the 30th European meeting of meat research workers, Bristol, pp. 394–396.Google Scholar
Moss, B. W., Hawe, S. M. and Walker, N. 1993. Sensory thresholds for skatole and indole. In Measurement and prevention of boar taint in entire male pigs (ed. Bonneau, M.), pp. 63–68. Institut National de la Recherche Agronomique, Paris.Google Scholar
Sheldon, B. W. and Essary, E. O. 1982. Effect of antibiotics on intestinal microflora and flavour of broiler meat. Poultry Science 61: 280–287.Google Scholar
Siret, F., Béague, M. P., Fischer, K. and Chevillon, P. 1997. Consumer acceptability and characterisation of cooking odour of pork with different androstenone and skatole contents: Comparison of two cooking procedures. In Boar taint in entire male pigs (ed. Bonneau, M., Lundström, K. and Malmfors, B.). Proceedings of a meeting of the EAAP working group ‘Production and Utilisation of Meat from Entire Male Pigs’, Stockholm, Sweden, 1-3 October 1997. European Association for Animal Production publication no. 92, pp. 161–164. Wageningen Pers.Google Scholar
Statistical Analysis Systems Institute. 1985, 1986 and 1987. SAS users’ guide, Statistical Analysis Systems Institute Inc., Cary, NC.Google Scholar
Urbach, G., Stark, W. and Forss, D. A. 1972. Volatile compounds in butter oil. Journal of Dairy Research 39: 35–47.Google Scholar