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Evaluación del semen criopreservado de toros Curraleiro Pé Duro, perteneciente al Banco Brasilero de Germoplasma Animal

Published online by Cambridge University Press:  01 May 2014

I.S. Montesinos ,
J.O. Carvalho ,
J.V. Malaquias ,
E. Arnhold ,
G.E. Freneau ,
M.A.N. Dode ,
M.C.S. Fioravanti  and
J.R.B. Sereno
I.S. Montesinos*
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
J.O. Carvalho
Affiliation:
Embrapa Cenargen, CEP 70770-900, Brasilia-DF, Brasil
J.V. Malaquias
Affiliation:
Embrapa Cerrados, CEP 73310-970, Planaltina-DF, Brasil
E. Arnhold
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
G.E. Freneau
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
M.A.N. Dode
Affiliation:
Embrapa Cenargen, CEP 70770-900, Brasilia-DF, Brasil
M.C.S. Fioravanti
Affiliation:
Universidad Federal de Goiás, CEP 74001-970. Goiânia-GO, Brasil
J.R.B. Sereno
Affiliation:
Embrapa Cerrados, CEP 73310-970, Planaltina-DF, Brasil
*
Correspondencia: I.S. Montesinos, Universidad Federal de Goiás, Brasil. e-mail: ivansm.vet@gmail.com
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Summary

This investigation aimed to evaluate the quality of cryopreserved semen from 21 Curraleiro Pé Duro bulls in a germplasm bank, categorizing the doses of the sires according to parameters related to sperm viability, and verifying the fertilizing capacity in vitro of the spermatozoids. All the samples were thawed and analyzed as to morphology, kinetics, plasma membrane integrity and acrosome integrity. Samples of only four bulls were used for evaluating the fertilization rates. The results indicated that the Curraleiro Pé Duro semen doses, stocked in the Brazilian Animal Germplasm Bank, showed variable sperm quality and high in vitro fertilizing capacity, making it possible to design appropriate strategies for assisted reproduction in order to conserve this Brazilian genetic resource, which is in danger of extinction.

Resumen

Esta investigación tuvo el objetivo de evaluar la calidad del semen criopreservado de 21 toros Curraleiro Pé Duro en banco de germoplasma, categorizando las dosis de los reproductores según parámetros relacionados a la viabilidad espermática, y verificando la capacidad fecundante in vitro de los espermatozoides. Todas las muestras fueron descongeladas y analizadas en cuanto a morfología, cinética, integridad de membrana plasmática e integridad de acrosoma. Para evaluar las tasas de fecundación, muestras de sólo cuatro toros fueron utilizadas. Los resultados indicaron que las dosis de semen Curraleiro Pé Duro, almacenadas en el Banco Brasilero de Germoplasma Animal, mostraron calidad espermática variable y alta capacidad fecundante in vitro, posibilitando delinear estrategias adecuadas de reproducción asistida para la conservación de este recurso genético brasilero en peligro de extinción.

Résumé

Cette recherche visait à évaluer la qualité du sperme cryoconservés de 21 taureaux Curraleiro Pé Duro dans une banques de gènes, categorisant les doses des reproduteurs selon les paramètres liés à la viabilité du sperme, et de vérifier la capacité fecondante in vitro des spermatozoïdes. Tous les échantillons ont été décongelés et analysés pour la morphologie, la cinétique et la membrane plasmique et intégrité de l'acrosome. Pour évaluer le taux de fecondation, des échantillons de seulement quatre taureaux ont été utilisés. Les résultats ont indiqués que les doses de sperme Curraleiro Pé Duro, stokée dans la Banque Bresilienne de Gènes Animal, ont montré de haute qualité du sperme variable capacité de fécondation in vitro, permettant délimiter les stratégies appropriés de reproduction assistée pour la préservation de cette ressource génétique bresilienne en voie de disparition.

Keywords

conservación ex situespermatozoiderazas criollascreole breedsex situ conservationspermatozoidcréoles racesla conservation ex situspermatozoïde
Type
Research Article
Information
Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales , Volume 54 , June 2014 , pp. 135 - 140
Copyright
Copyright © Food and Agriculture Organization of the United Nations 2014 

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