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A study of familial aspects of human urinary amino acid excretory patterns utilizing paper chromatography1

Published online by Cambridge University Press:  01 August 2014

Arnold R. Kaplan*
Affiliation:
Department of Zoology, University of Texas, Austin

Summary

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A method for the preliminary analysis of a maximal number of urinary amino acids has been devised, which is based on preliminary ‘purification’ and desalting of each specimen prior to its subjection to two-dimensional paper chromatography. Aliquots of processed and concentrated specimens are standardized to quantities of alpha-amino acid nitrogen as determined colorimetrically.

Densitometric index values were analyzed for sex differences and for intrapair differences comparing genetically related and unrelated pairs. Significant sex differences were observed for five substances. Significant deviations between intrapair differences of genetically-related and control pairs were observed for ten substances. The results are compatible with the hypothesis that quantitative differences in the respective excretions involve genetic factors.

Riassunto

RIASSUNTO

È stato ideato un metodo per l'analisi preliminare del massimo numero di aminoacidi urinari, metodo che si basa su di una purificazione e desalinizzazìone preliminare di ogni campione prima di sottoporlo alla cromatografia bidirezionale su carta. Aliquote di campioni preparati e concentrati vengono standardizzate rispetto a quantità di azoto alfa-aminoacido mediante determinazione colorimetrica.

I valori dell'indice densimetrico sono stati analizzati rispetto alle differenze di sesso ed alle differenze intracoppia confrontando coppie geneticamente imparentate e non imparentate. Sono state trovate differenze significative tra i sessi per cinque sostanze. I risultati sono compatibili con l'ipotesi che le differenze quantitative nelle rispettive escrezioni comportino fattori genetici.

Résumé

RÉSUMÉ

On a suggeré une méthode pour l'analyse préalable du plus grand nombre d'aminoacides urinaires, méthode qui est fondée sur une purification et un dessalage préalable de chaque spécimen avant de le soumettre à la Chromatographie bidirectionnelle sur papier. Des aliquotes des spécimens préparés et concentrés sont standardisés par rapport à quantités d'azote alfa-aminoacide par détermination colori-métrique.

Les valeurs de l'index densimétrique ont été analisées, pour les différences de sexe et les différences intracouple, par la comparaison de couples génétiquement apparentées et non-apparentées. On a trouvé de différences significatives entre les sexes pour cinq substances.

Les résultats sont compatibles avec l'hypothèse que les différences quantitatives des excrétions respectives comportent des facteurs génétiques.

Zusammenfassung

ZUSAMMENFASSUNG

Es wurde eine Methode erdacht für die Voranalyse der Höchstzahl an Aminosäuren im Urin. Dabei wurd zuerst jedes Muster, bevor es mittels Papierchromatographie in zwei Richtungen untersucht wird, gereinigt und entsalzt. Aliquoten präparierter und konzentrierter Muster werden durch Kolorimetrie auf ihren alpha-aminosauren Stickstoffgehalt standardisiert.

Die Werte des Densimeterindexes wurden auf Geschlechtsunterschiede und auf die Differenzen zwischen genetisch verwandten und nicht-verwandten Paaren untersucht. Für fünf Stoffe wurden bezeichnende Unterschiede zwischen den Geschlechtern gefunden. Die Ergebnisse decken sich mit der Annahme, dass die quantitativen Unterschiede in den verschiedenen Ausscheidungen genetische Faktoren enthalten.

Type
Research Article
Copyright
Copyright © The International Society for Twin Studies 1959

Footnotes

1

From a dissertation (Kaplan, 1956) completed in partial fulfillment of the requirements for the Ph. D. degree at the University of Texas, Austin.

References

Fister, H. J.: 1950. Manual of Standardized Procedures for Spectrophotometric Chemistry. Easton, Pa.: Mack Ptg. Co. Google Scholar
Gartler, S. M., Dobzhansky, T., and Berry, H. K.: 1955. Chromatographic studies of urinary excretion patterns in monozygotic and dizygotic twins. II. Heritability of the excretion rates of certain substances. Am. J. Human Genet., 7: 108121.Google ScholarPubMed
Kaplan, A. R.: 1956. Chromatographic studies of genetic aspects of human urinary amino acid excretory patterns. Ph. D. dissertation, University of Texas, Austin.Google Scholar
Williams, R. J., et al.: 1951. Biochemical Institute Studies. IV. Univ. Tex. Pub. No. 5109. Austin: Univ. Texas Press.Google Scholar