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Assessment of the potential to improve N fixation by cowpea (Vigna unguiculata (L.) Walp.) in Ghanaian soils

  • J.O. Fening (a1), W. Dogbe (a2) and S.K.A. Danso (a3)

Abstract

The potential to improve N fixation by cowpea in Ghanaian soils was examined through: (1) assessment of the natural nodulation of 45 cowpea cultivars in 20 soils sampled from 5 ecological zones; (2) determination of the numbers of cowpea bradyrhizobial isolates in the soils; and (3) determination of the response of cowpea to N fertilization. The ability of 45 cowpea cultivars to nodulate naturally in the various soils showed wide cultural adaptability. Counts of indigenous bradyrhizobia showed that most soils in Ghana contained large populations capable of nodulating cowpea. These ranged from 0.6 × 10 bradyrhizobia cells g soil−1 to 3.1 × 104 cells g soil−1, with 60% of the soils containing more than 103 cells g soil−1. Response of cowpea to N fertilization differed according to soil type. In general all cowpea cultivars showed significant response to increasing N fertilizer applications, indicating that N fixation was not providing the plants with sufficient N for maximum growth and yield. This study suggests that inoculation of cowpea with effective indigenous strains of bradyrhizobial species has considerable potential to improve this situation.

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Corresponding author

Corresponding author is J.O. Fening (soils@africaonline.com.gh).

References

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1.Awonaike, K.O., Kumarasinghe, K.S., and Danso, S.K.A.. 1990. Nitrogen fixation and yield of cowpea (Vigna unguiculata) as influenced by cultivar and Bradyrhizobium strain. Field Crops Res. 24:163171.
2.Brammer, H. 1962. Soils of Ghana. In Wills, J.B. (ed.). Agriculture and Land Use in Ghana. Oxford University Press, London, p. 88126.
3.Bray, R.H., and Kurtz, L.T.. 1945. Determination of total, organic and available forms of phosphorus in soils. Soil Sci. 59:3945.
4.Bremner, J.M. 1960. Determination of nitrogen in soil by Kjeldahl method. J. Agric. Sci. 55:1133.
5.Bumb, B.L. 1995. World nitrogen supply and demand: An overview. In Bacon, P.E. (ed.). Nitrogen Fertilization in the Environment. Marcel Dekker, New York. p. 101103.
6.Buresh, R.J., Sanchez, P.A., and Calhoun, F.G. (eds.). 1997. Replenishing Soil Fertility in Africa. SSSA Spec. Pub. 51. Soil Science Society of America and American Society of Agronomy, Madison, WI.
7.Carroll, B.J., and Gresshoff, P.M.. 1983. Nitrate inhibition of nodulation and nitrogen fixation in white clover. Z. Pflanzenphysiol. 110:7788.
8.Carroll, B.J., and Mathews, A.. 1990. Nitrate inhibition of nodulation in legumes. In Gresshoff, P.M. (ed.). Molecular Biology of Symbiotic Nitrogen Fixation. CRC Press, Boca Raton, FL. p. 159180.
9.Chapman, A.L., and Myers, R.J.K.. 1987. N2 contributed by grain legumes in rotation with rice on the Cununura soils of the Ord irrigation area, Western Australia. Aust. J. Exp. Agric. 27:155163.
10.Cregan, P.B., Keyser, H.H., and Sadowsky, M.J.. 1989. Host plant effects on nodulation and competitiveness of the Bradyrhizobium japonicum serotype strains constituting serocluster 123. Appl. Environ. Microbiol. 55:25322536.
11.Danso, S.K.A., and Alexander, M.. 1974. Survival of two Rhizobium strains in soil. Soil Sci. Soc. Amer. Proc. 39:369372.
12.Eardly, B.D., Hannaway, D.B., and Bottomley, P.J.. 1984. Nitrogen nutrition and yield of seedling alfalfa as affected by ammonium nitrate fertilization. Agron. J. 77:5762.
13.George, T., Ladha, J.K., Buresh, R.J., and Garrity, D.P.. 1992. Managing native and legume-fixed N2 in lowland rice-based cropping systems. Plant Soil 141:6991.
14.Hardarson, G., Golbs, M., and Danso, S.K.A.. 1989. Nitrogen fixation in soybean (Glycine max L. Merrill) as affected by nodulation patterns. Soil Biol. Biochem. 21:783787.
15.Hardarson, G., Zapata, F., and Danso, S.K.A.. 1984. Effects of plant genotype and nitrogen fertilizer on symbiotic nitrogen fixation by soybean cultivars. Plant Soil 82:397405.
16.Marschner, H. 1986. Mineral Nutrition of Higher Plants. Academic Press, New York. p. 195200.
17.Munns, D.N., and Keyser, H.H.. 1981. Tolerance of rhizobia to acidity and phosphate. Soil. Sci. Soc. Amer. J. 34:519523.
18.Peoples, M.B., and Craswell, E.T.. 1992. Biological nitrogen fixation: Investments, expectations and actual contributions to agriculture. Plant Soil 141:1339.
19.Peoples, M.B., Herridge, D.F., and Ladha, J.K.. 1995. Biological nitrogen fixation: An efficient source of nitrogen for sustainable agricultural production? Plant Soil 174:328.
20.Ruschel, A.P., Salati, E., and Vose, P.B.. 1979. Nitrogen enrichment of soil and plant by Rhizobium phaseoli-Phaseolus vulgaris symbiosis. Plant Soil 51:425429.
21.Senaratne, R., Amornpinol, C., and Hardarson, G.. 1987. Effect of combined nitrogen on nitrogen fixation of soybean (Glycine max (L.) Merrill) as affected by cultivar and rhizobial strain. Plant Soil 103:4550.
22.Somasegaran, P., and Hoben, H.J.. 1994. Handbook for Rhizobia: Methods in Legume-Rhizobium Technology. Springer-Verlag, Heidelberg, Germany.
23.Stoorvogel, J.J., Smaling, E.M.A., and Janssen, B.H.. 1993. Calculating soil nutrient balances in Africa at different scales. I. Supra-national scale. Fert. Res. 35:227235.
24.Streeter, J.G. 1985. Nitrate inhibition of legume nodule growth and activity II. Short-term studies with high nitrate supply. Plant Physiol. 77:325328.
25.Thies, J.E., Singleton, P.W., and Bohlool, B.B.. 1991. Influence of the size of indigenous rhizobial populations on establishment and symbiotic performance of introduced rhizobia on field-grown legumes. Appl. Environ. Microbiol. 57:1928.
26.USDA. 1975. Soil Taxonomy: A Basic System of Soil Classification for Making and Interpreting Soil Surveys. Agric. Handbook 436. U.S. Dept. of Agriculture, Soil Conservation Service, Washington, DC.
27.Vincent, J.M. 1970. A Manual for the Practical Study of Root Nodule Bacteria. International Biological Programme Handbook. Blackwell Scientific, Oxford, U.K. p. 7379.
28.Vincent, J.M. 1980. Factors controlling the legume-Rhizobium symbiosis. In Newton, W.E. and Orme-Johnson, W.H. (eds.). Nitrogen Fixation. Vol. 2. Symbiotic Associations and Cyanobacteria. University Park Press, Baltimore, MD. p. 101129.
29.Walkley, A., and Black, C.A.. 1934. An examination of the Degtijareff method for determining soil organic matter and a proposed modification of the chromic and titration method. Soil Sci. 37:2938.
30.Weaver, R.W., and Frederick, L.R.. 1972. Effect of inoculum size on nodulation of Glycine max (L.) Merrill, variety Ford. Agron. J. 64:597599.
31.Woomer, P., Singleton, P.W., and Bohlool, B.B.. 1988. Ecological indicators of native rhizobia in tropical soils. Appl. Environ. Microbiol. 54:11121116.

Keywords

Assessment of the potential to improve N fixation by cowpea (Vigna unguiculata (L.) Walp.) in Ghanaian soils

  • J.O. Fening (a1), W. Dogbe (a2) and S.K.A. Danso (a3)

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