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Increased GABAA receptor binding in amygdala after prenatal administration of valproic acid to rats

  • Freja Bertelsen (a1) (a2), Arne Møller (a1) (a2), Davide Folloni (a1) (a3), Kim Ryun Drasbek (a1), Jørgen Scheel-Krüger (a1) and Anne M. Landau (a2) (a4)...

Abstract

Objective

Prenatal exposure to valproic acid (VPA) enhances the risk for later development of autism spectrum disorders (ASD). An altered gamma-aminobutyric acid (GABA) system may be a key factor in ASD. Here we investigated possible changes in the GABA system in rats exposed to a low dose of prenatal VPA.

Method

We performed autoradiography with [3H]muscimol, (a GABAA receptor agonist), and [11C]Ro15-4513 (a partial agonist of the GABAA α1+5 receptor subtypes), in brain sections containing amygdala, thalamus and hippocampus of rats treated prenatally with 20 mg/kg VPA or saline from the 12th day of gestation.

Result

Prenatal VPA significantly increased [11C]Ro15-4513 binding in the left amygdala compared with controls (p<0.05). This difference was not observed in the hippocampus, thalamus or right amygdala. No differences were observed in [3H]muscimol binding.

Conclusion

We observed an asymmetric increase in GABAA receptor binding. Disturbances in the GABAA receptor system have also been detected in human autism with [11C]Ro15-4513.

Copyright

Corresponding author

Anne M. Landau, Department of Nuclear Medicine and PET Centre, Nørrebrogade 44, Building 10G, 8000 Aarhus, Denmark. Tel: +45 7846 3029; Fax: +45 7846 1662; E-mail: alandau@clin.au.dk

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Acta Neuropsychiatrica
  • ISSN: 0924-2708
  • EISSN: 1601-5215
  • URL: /core/journals/acta-neuropsychiatrica
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