Book contents
- Fertility Preservation
- Fertility Preservation
- Copyright page
- Dedication
- Contents
- Contributors
- Foreword
- Foreword
- Preface
- Section 1 Introduction
- Section 2 Reproductive Biology and Cryobiology
- Section 3 Fertility Preservation in Cancer and Non-Cancer Patients
- Section 4 Fertility Preservation Strategies in the Male
- Section 5 Fertility Preservation Strategies in the Female: Medical/Surgical
- Section 6 Fertility Preservation Strategies in the Female: ART
- Section 7 Ovarian Cryopreservation and Transplantation
- Section 8 In Vitro Follicle Culture
- Chapter 28 Molecular and Cellular Integrity of Cultured Follicles
- Chapter 29 In Vitro Growth of Human Oocytes
- Chapter 30 Contributions of Ovarian Stromal Cells to Follicle Culture
- Chapter 31 In Vitro Maturation of Germinal Vesicle Oocytes
- Chapter 32 Survival of Primordial Follicles
- Section 9 New Research and Technologies
- Section 10 Ethical, Legal, and Religious Issues
- Index
- References
Chapter 31 - In Vitro Maturation of Germinal Vesicle Oocytes
from Section 8 - In Vitro Follicle Culture
Published online by Cambridge University Press: 27 March 2021
- Fertility Preservation
- Fertility Preservation
- Copyright page
- Dedication
- Contents
- Contributors
- Foreword
- Foreword
- Preface
- Section 1 Introduction
- Section 2 Reproductive Biology and Cryobiology
- Section 3 Fertility Preservation in Cancer and Non-Cancer Patients
- Section 4 Fertility Preservation Strategies in the Male
- Section 5 Fertility Preservation Strategies in the Female: Medical/Surgical
- Section 6 Fertility Preservation Strategies in the Female: ART
- Section 7 Ovarian Cryopreservation and Transplantation
- Section 8 In Vitro Follicle Culture
- Chapter 28 Molecular and Cellular Integrity of Cultured Follicles
- Chapter 29 In Vitro Growth of Human Oocytes
- Chapter 30 Contributions of Ovarian Stromal Cells to Follicle Culture
- Chapter 31 In Vitro Maturation of Germinal Vesicle Oocytes
- Chapter 32 Survival of Primordial Follicles
- Section 9 New Research and Technologies
- Section 10 Ethical, Legal, and Religious Issues
- Index
- References
Summary
The field of female fertility preservation is based on the ability to successfully cryopreserve ovarian tissue [1], and this can now be offered to a wide range of patients worldwide [2, 3]. Fragments of cryopreserved ovarian cortex can be thawed and autografted to an orthotopic or heterotopic site to restore fertility [4, 5]. The potential of this tissue to restore fertility would be greatly enhanced if immature oocytes contained within the tissue could be grown to mature stages within the laboratory and this would be particularly beneficial for prepubertal girls who currently have fewer options to preserve and restore their fertility than adult women [2, 3].
Cryopreserved tissue contains the most immature stage of oocyte within primordial follicles and the main aim of culturing this tissue is to support in vitro gametogenesis/growth (IVG) to develop immature oocytes entirely in vitro [6]. If this methodology could be demonstrated to be safe, it would maximize the potential of cryopreserved ovarian tissue and have many clinical applications [2, 3].
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- Information
- Fertility PreservationPrinciples and Practice, pp. 355 - 363Publisher: Cambridge University PressPrint publication year: 2021