Estienne still worried in 1562 that if words lost their meanings, however conventionally established, the processes of language and learning became nonsense, decaying into madness:

He tied this unreason directly to political conditions, arguing that bringing Machiavellian political practices into France had fatally undermined linguistic, and so conceptual, sureties. He blamed the matter on those who frequented the Medici-controlled court, and in this we may well see a continuation of that path of civic humanism to which I have already several times referred.

Like most others in France, as religious disturbances in the 1540s and fifties became increasingly vicious civil dynastic wars in the 1560s and seventies, Estienne grew warmer on the subject. By 1575, he had joined the Protestant polemicist Innocent Gentillet in directly attacking the queen: Discours merveilleux de la vie, actions & deportemens de Catherine de Medicis royne mere.