Skip to main content Accessibility help
×
Hostname: page-component-848d4c4894-r5zm4 Total loading time: 0 Render date: 2024-06-24T23:56:05.219Z Has data issue: false hasContentIssue false

12 - Cryopreservation of blastocysts

from Cryopreservation of embryos

Published online by Cambridge University Press:  06 July 2010

Ri-Cheng Chian
Affiliation:
McGill University, Montréal
Get access

Summary

Cryopreservation techniques for blastocysts are required to preserve surplus blastocysts. Cryopreservation of the blastocyst was mainly carried out with a slow-freezing technique using programmed freezers. As a typical container for embryos, a cryostraw, includes both liquid (cryoprotectant) and solids, a slow change in temperature is needed to preserve cell structure. The vitrification method developed for mammals has recently been applied to human embryos. The chapter outlines a vitrification procedure for cryopreservation of blastocysts (Cryotop method). The cryopreservation of surplus blastocysts is beneficial, especially for day 6 blastocysts. When cryopreserved and thawed blastocysts are transferred into the uterus, two methods of endometrial preparation for transfer are available. One is transferring to the endometrium in the natural menstrual cycle and the other is to an endometrium prepared by hormone replacement. For patients who have a chromosomal abnormality, preimplantation genetic diagnosis (PGD) is applied for in order to choose normal embryos.
Type
Chapter
Information
Publisher: Cambridge University Press
Print publication year: 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Save book to Kindle

To save this book to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×