A Palmer amaranth biotype (CT-Res) with resistance to glyphosate was recently confirmed in a pumpkin field in Connecticut. However, the underlying mechanisms conferring glyphosate resistance in this biotype is not known. The main objectives of this research were 1) to determine the effect of plant height (10, 20, and 30 cm) on glyphosate resistance levels in CT-Res Palmer amaranth biotype, and 2) to investigate whether the target site–based mechanisms confer glyphosate resistance. To achieve these objectives, progeny seeds of the CT-Res biotype after two generations of recurrent selection with glyphosate (6,720 g ae ha−1) were used. Similarly, known glyphosate-susceptible Palmer amaranth biotypes from Kansas (KS-Sus) and Alabama (AL-Sus) were included. Results from greenhouse dose-response studies revealed that CT-Res Palmer amaranth biotype had 69-, 64-, and 54-fold resistance to glyphosate as compared with the KS-Sus biotype when treated at heights of 10, 20, and 30 cm, respectively. Sequence analysis of the EPSPS gene revealed no point mutations at the Pro106 and Thr102 residues in the CT-Res Palmer amaranth biotype. Quantitative polymerase chain reaction analysis revealed that the CT-Res biotype had 33 to 111 relative copies of the EPSPS gene compared with the AL-Sus biotype. All these results suggest that the EPSPS gene amplification endows a high level of glyphosate resistance in the GR Palmer amaranth biotype from Connecticut. Because of the lack of control with glyphosate, growers should adopt the use of effective alternative preemergence and postemergence herbicides in conjunction with other cultural and mechanical tactics to mitigate the further spread of GR Palmer amaranth in Connecticut.

Waterhemp has evolved resistance to seven herbicide modes of action in the United States and to five in Canada, which limits weed control options for producers. The objective of this research was to quantify the level and duration of residual control of multiple herbicide-resistant (MHR) waterhemp with five Group 15 herbicides (acetochlor, dimethenamid-p, flufenacet, pyroxasulfone, and S-metolachlor) applied preemergence in a non-crop area. Four field trials were conducted over a 2-yr period (2021, 2022) in southwestern Ontario, Canada. By 4 wk after application (WAA) 91% of waterhemp had emerged in the nontreated control area. The numerical control of waterhemp with all Group 15 herbicides, with the exception of pyroxasulfone, was greatest at 4 WAA, then control declined. Flufenacet provided the lowest waterhemp control; dimethenamid-p and S-metolachlor provided intermediate control, and acetochlor and pyroxasulfone provided the highest control. Waterhemp control with pyroxasulfone peaked at 6 WAA with 99% and declined to 77% at 12 WAA. Flufenacet (low and high rates) was predicted to reduce waterhemp emergence by 50% for 42 to 44 d after application (DAA). Dimethenamid-p, S-metolachlor, and acetochlor (both formulations and three rates) were predicted to reduce waterhemp emergence by 80% for 36, 43, and 33 to 51 DAA, respectively; in contrast, pyroxasulfone was predicted to reduce waterhemp emergence by 80% for 82 DAA. This study concludes that of the Group 15 herbicides evaluated, flufenacet provides the lowest and shortest residual control of waterhemp, and pyroxasulfone provides the highest and longest residual control of waterhemp.

Palmer amaranth (Amaranthus palmeri S. Watson) is not native to Africa. Based on the presence and persistence of A. palmeri populations, its invasive status in southern Africa is classified as “naturalized.” Globally, A. palmeri is one of the most troublesome weed species in several crops, including soybean [Glycine max (L.) Merr.], maize (Zea mays L.), and cotton (Gossypium hirsutum L.). Certain populations of A. palmeri in various countries were reported to be resistant to herbicides with different sites of action (SOAs). Two biotypes of A. palmeri in the United States reportedly each have resistance to herbicides representing five different SOAs, and between them a total of eight different SOAs are involved. Resistance mechanisms in these biotypes involve target-site and/or non–target site resistance. Here we characterize a specific A. palmeri population that was found in the Douglas district in South Africa and showed resistance to various herbicide SOAs. Initially, this A. palmeri population was discovered in a glyphosate-tolerant cotton field, where it survived glyphosate treatment. Subsequently, greenhouse experiments were conducted to characterize this A. palmeri population for potential resistance to herbicides of additional SOAs, and molecular analyses were conducted to reveal the mechanisms of herbicide resistance. Results indicated resistance to chlorimuron-ethyl and glyphosate in this population, while <90% control (decreased sensitivity) was observed at the label rate for mesotrione, atrazine, saflufenacil, and S-metolachlor. However, glufosinate, tembotrione, acifluorfen, dicamba, 2,4-D, metribuzin, acetochlor, isoxaflutole, diflufenican, and pyroxasulfone were effective at controlling this population. This profiling of herbicide sensitivity has allowed development of programs to control and potentially minimize the spread of this weed. In addition, molecular analysis of EPSPS revealed the role of higher copy number as a mechanism for glyphosate resistance in this population and a Ser-653-Asn target-site mutation likely conferring resistance to the acetolactate synthase–inhibitor chlorimuron-ethyl. No known target-site mutations were identified for the protoporphyrinogen oxidase–inhibitor group.

Failure to control Palmer amaranth with glyphosate and protoporphyrinogen IX oxidase (PPO)-inhibitor herbicides was reported across southwestern Nebraska in 2017. The objectives of this study were to 1) confirm and 2) validate glyphosate and PPO-inhibitor (fomesafen and lactofen) resistance in 51 Palmer amaranth accessions from southwestern Nebraska using genotypic and whole-plant phenotypic assay correlations and cluster analysis, and 3) determine which agronomic practices might be influencing glyphosate resistance in Palmer amaranth accessions in that location. Based on genotypic assay, 88% of 51 accessions contained at least one individual with amplification (>2 copies) of the 5-enolypyruvyl-shikimate-3-phosphate synthase (EPSPS) gene, which confers glyphosate resistance; and/or a mutation in the PPX2 gene, either ΔG210 or R128G, which endows PPO-inhibitor resistance in Palmer amaranth. Cluster analysis and high correlation (0.83) between genotypic and phenotypic assays demonstrated that EPSPS gene amplification is the main glyphosate resistance mechanism in Palmer amaranth accessions from southwestern Nebraska. In contrast, there was poor association between genotypic and phenotypic responses for PPO-inhibitor resistance, which was attributed to segregation for PPO-inhibitor resistance within these accessions and/or the methodology that was adopted herein. Genotypic assays can expedite the process of confirming known glyphosate and PPO-inhibitor resistance mechanisms in Palmer amaranth from southwestern Nebraska and other locations. Phenotypic assays are also a robust method for confirming glyphosate resistance but not necessarily PPO-inhibitor resistance in Palmer amaranth. Moreover, random forest analysis of glyphosate resistance in Palmer amaranth indicated that EPSPS gene amplification, county, and current and previous crops are the main factors influencing glyphosate resistance within that geographic area. Most glyphosate-susceptible Palmer amaranth accessions were found in a few counties in areas with high crop diversity. Results presented here confirm the spread of glyphosate resistance and PPO-inhibitor resistance in Palmer amaranth accessions from southwestern Nebraska and demonstrate that less diverse cropping systems are an important driver of herbicide resistance evolution in Palmer amaranth.

Pigweed is difficult to manage in grain sorghum because of widespread herbicide resistance, a limited number of registered effective herbicides, and the synchronous emergence of pigweed with grain sorghum in Kansas. The combination of cultural and mechanical control tactics with an herbicide program are commonly recognized as best management strategies; however, limited information is available to adapt these strategies to dryland systems. Our objective for this research was to assess the influence of four components, including a winter wheat cover crop (CC), row-crop cultivation, three row widths, with and without a herbicide program, on pigweed control in a dryland system. Field trials were implemented during 2017 and 2018 at three locations for a total of 6 site-years. The herbicide program component resulted in excellent control (>97%) in all treatments at 3 and 8 weeks after planting (WAP). CC provided approximately 50% reductions in pigweed density and biomass for both timings in half of the site-years; however, mixed results were observed in the remaining site-years, ranging from no attributable difference to a 170% increase in weed density at 8 WAP in 1 site-year. Treatments including row-crop cultivation reduced pigweed biomass and density in most site-years 3 and 8 WAP. An herbicide program is required to achieve pigweed control and should be integrated with row-crop cultivation or narrow row widths to reduce the risk of herbicide resistance. Additional research is required to optimize the use of CC as an integrated pigweed management strategy in dryland grain sorghum.

Field experiments were conducted in 2013, 2014, and 2015 in Columbia and Moberly, Missouri to determine the effects of cereal rye, Italian ryegrass, winter wheat, winter oat, crimson clover, Austrian winterpea, hairy vetch, oilseed radish, and cereal rye plus hairy vetch on winter and summer annual weed emergence in soybean. For comparison purposes, each experiment in each year included a Fall PRE, Spring PRE without residual, and Spring PRE residual herbicide programs. Cereal rye and cereal rye plus hairy vetch reduced winter annual weed emergence by 72 and 68%, respectively, but were not comparable to the Fall PRE which reduced winter annual weed emergence by 99%. The following spring, early-season waterhemp emergence was similar among treatments of cereal rye, cereal rye plus hairy vetch, and the Spring PRE residual herbicide program. In contrast, all cover crop species other than Italian ryegrass reduced late season waterhemp emergence between 21 and 40%, but were not comparable to the Spring PRE residual herbicide program, which reduced late season waterhemp emergence by 97%. All other summer annual weeds excluding waterhemp showed a similar response among cover crop and herbicide treatments. Overall, results from this experiment indicate that certain cover crops are able to suppress winter and summer annual weed emergence, but not to the extent of soil-applied residual herbicides.

Conservation agriculture (CA) practices are threatened by glyphosate-resistant Palmer amaranth. Integrated control practices including PRE herbicides and high-residue CA systems can decrease Amaranthus emergence. Field experiments were conducted from autumn 2006 through crop harvest in 2009 at two sites in Alabama to evaluate the effect of integrated weed management practices on Amaranthus population density and biomass, cotton yield, and economics in glyphosate-resistant cotton. Horizontal strips included four CA systems with three cereal rye cover crop seeding dates and a winter fallow (WF) CA system compared to a conventional tillage (CT) system. Additionally, vertical strips of four herbicide regimes consisted of: broadcast, banded, or no PRE applications of S-metolachlor (1.12 kg ai ha−1) followed by (fb) glyphosate (1.12 kg ae ha−1) applied POST fb layby applications of diuron (1.12 kg ai ha−1) plus MSMA (2.24 kg ai ha−1) or the LAYBY application alone. Early-season Amaranthus density was reduced in high-residue CA in comparison to the CA WF systems in 2 of 3 yr. Amaranthus densities in herbicide treatments that included a broadcast PRE application were lower at three of five sampling dates compared to banding early-season PRE applications; however, the differences were not significant during the late season and cotton yields were not affected by PRE placement. High-residue conservation tillage yields were 577 to 899 kg ha−1 more than CT, except at one site in 1 yr when CT treatment yields were higher. CA utilizing high-residue cover crops increased net returns over CT by $100 ha−1 or more 2 out of 3 yr at both locations. High-residue cover crop integration into a CA system reduced Amaranthus density and increased yield over WF systems; the inclusion of a broadcast PRE application can increase early-season Amaranthus control and might provide additional control when glyphosate-resistant Amaranthus populations are present.

Glyphosate-resistant Palmer amaranth has become a major problem for cotton producers throughout much of the southern United States. With cotton producers relying heavily on glyphosate-resistant cotton, an alternative solution to controlling resistant Palmer amaranth is needed. A field experiment was conducted during 2009 and 2010 at Marianna, AR, in which a rye cover crop and no cover crop were tested in combination with deep tillage with the use of a moldboard plow and no tillage to determine the impact on Palmer amaranth emergence in cotton. To establish a baseline population, 500,000 glyphosate-resistant Palmer amaranth seeds were placed in a 2-m2 area in the middle of each plot and incorporated into the soil, and emergence was evaluated five times during the season. In 2009, both tillage and the cover crop reduced Palmer amaranth emergence in cotton, but the combination of the two reduced emergence 85%. In the second year, only the cover crop reduced Palmer amaranth emergence in cotton, a 68% reduction. Cover crops and deep tillage will not eliminate glyphosate-resistant Palmer amaranth; however, use of these tools will likely reduce the risks of failures associated with residual herbicides along with selection pressure placed on both PRE- and POST-applied herbicides. Additional efforts should focus on the integration of the best cultural practices identified in this research with use of residual herbicides and greater focus on limiting Palmer amaranth seed production and reducing the soil seedbank.

Herbicide-resistant Palmer amaranth has become the most economically detrimental weed of cotton in the southeast United States. With the continual marginalization of potential herbicide tools, research has expanded to include alternative means of affecting future Palmer amaranth populations by altering safe sites and reducing inputs to the seedbank population. The influence of delayed Palmer amaranth establishment on seed production potential has not been investigated in the southeast United States. Studies were conducted to evaluate the influence of time of Palmer amaranth establishment on morphology, growth, and seed production. The experiment was a factorial, with five levels of Palmer amaranth transplanting (0 to 12 wk after cotton planting) and two levels of crop type (cotton and noncrop). In the absence of crop competition, the first cohort of Palmer amaranth produced 446,000 seeds per plant. This potential seed production was reduced 50% when Palmer amaranth plants were established nearly 6 wk later. In contrast, the first Palmer amaranth cohort growing in competition with cotton produced 312,000 seeds, 30% less than was produced in the absence of competition. Interference from cotton shifted time to 50% seed production to nearly 4 wk earlier in the growing season, relative to plants without crop competition. Delayed establishment of Palmer amaranth simulates premature loss of herbicide efficacy and alters the competitive balance between cotton and Palmer amaranth. Although the planting time that resulted in 50% reduction in Palmer amaranth plant height at the end of the season was not affected by the presence of cotton, 50% reduction in plant width and biomass occurred within the first 3.8 wk of the growing season when growing with cotton, more than 4 wk earlier than Palmer amaranth growing without a crop. This suggests that early season weed control programs will have a large effect on Palmer amaranth potential growth and seed production, as well as crop yield. To implement soil seedbank reduction strategies effectively, information on mechanisms that regulate Palmer amaranth persistence in the soil seedbank is needed.

Glyphosate-resistant Palmer amaranth has become a major threat to soybean production in the southern United States. Arkansas soybean producers rely heavily on glyphosate-resistant soybean; hence, an alternative solution for controlling resistant Palmer amaranth is needed. A field experiment was conducted at Marianna, AR, during 2009 and 2010 in which soybean production systems were tested in combination with deep tillage and no tillage to determine the impact on Palmer amaranth emergence. To establish a baseline population, 250,000 glyphosate-resistant Palmer amaranth seeds were placed in a 1-m2 area in the middle of each plot and incorporated in the soil, and emergence was evaluated five times during the season. Production systems of full-season soybean with a rye cover crop or soybean double-cropped with wheat, which had high amounts of plant residue on the soil surface reduced Palmer amaranth emergence more than systems without deep tillage and a cover crop or wheat. When used in combination with deep tillage, these systems reduced Palmer amaranth emergence by 98 and 97%, respectively, in 2009 and by 73 and 82%, respectively, in 2010. Deep tillage alone caused an 81% reduction in emergence averaged over both years. Soybean double-cropped with wheat used in combination with deep tillage provided a 95% reduction in Palmer amaranth emergence over the 2-yr period. This research shows that deep tillage in combination with soybean production systems that have high amounts of residue on the soil surface are alternative means for providing a high level of control of glyphosate-resistant Palmer amaranth and could lessen the dependence on chemical weed control.

A 2-yr field study was conducted to compare the growth of Amaranthus palmeri, A. rudis, A. retroflexus, and A. albus planted in June and July. Rates of height increase (centimeters per growing degree day) were 0.21 and 0.18 for A. palmeri, 0.16 and 0.11 for A. rudis, 0.12 and 0.09 for A. retroflexus, and 0.08 and 0.09 for A. albus in 1994 and 1995, respectively, when planted in June. A. palmeri had among the highest values for plant volume, dry weight, and leaf area, while A. albus had the lowest. Specific leaf area values (cm2 g−1) ranged from 149 to 261 for A. palmeri, 160 to 205 for A. rudis, 150 to 208 for A. retroflexus, and 127 to 190 for A. albus. Maximum relative growth rates (g g−1 day−1) for any measured period were 0.32 for A. palmeri, 0.31 for A. rudis, 0.30 for A. retroflexus, and 0.26 for A. albus. Recent increases in species range and observed changes in weed community structure may be partially explained by the growth characteristics of A. palmeri and A. rudis. Herbicide rate and timing recommendations for mixed populations of these weeds should be based on A. palmeri because of its high growth rates.

Field experiments were conducted in 2012 and 2013 to determine the effects of row spacing, seeding rate, and herbicide programs on multiple-resistant waterhemp control and yield in glufosinate-resistant soybean. The two herbicide programs evaluated were: (1) a PRE application of fomesafen plus S-metolachlor followed by early POST application of glufosinate plus acetochlor, referred to as the PRE followed by (fb) POST with residual (w/RES) herbicide program; and (2) an early POST followed by a late POST application of glufosinate, referred to as the two-pass POST herbicide program. Results indicate that the PRE fb POST w/RES program provides greater control of resistant waterhemp compared to the two-pass POST herbicide program. In 2012, the PRE fb POST w/RES program resulted in a 99% waterhemp density reduction and 156 kg ha−1 increase in yield compared to the 72% density reduction by the two-pass POST program. In 2013, the two-pass POST program was equally as effective on density reduction and yield as the PRE fb POST w/RES program. Waterhemp control and density reduction was always greatest with 19- and 38- compared to 76-cm rows. In 2012, the PRE fb POST w/RES program provided at least 95% control and greater than 98% density reduction across all row spacings, whereas the two-pass POST program provided 95%, 95%, and 85% control and 87%, 80%, and 50% density reduction in 19-, 38-, and 76-cm rows, respectively. Soybean seeding rate did not affect waterhemp control or density in either year. In both years, 165,000 seeds ha−1 yielded lower than the three higher seeding rates. Overall, results from these experiments indicates that the use of a PRE fb POST w/RES program, narrow-row spacing, and seeding rates of 240,000 to 315,000 seeds ha−1 or greater provides the greatest waterhemp control, density reduction, and soybean yield when multiple resistant waterhemp is present.

Interspecific hybridization of Palmer amaranth and common waterhemp produce hybrids with unique DNA fragments not found in either parent. The objective of this research was to investigate the mechanisms involved in the formation of the polymorphic fragments. Six novel fragments were cloned and sequenced. Five of the six were significantly similar to plant transposons, the sixth was similar to squamosa promoter–binding proteins from other plant species. Southern blot analysis using one of the novel fragments as probe revealed a consistent pattern of repetitive DNA that was species and biotype specific. These results indicate that transposon-like elements may play an important role in the formation of new fragments in Amaranthus hybrids derived from interspecific hybridization, suggesting that considerable instability of the hybrid genome may occur.

We monitored emergence of morning-glory, pigweed, and prickly sida from seeded populations in no-till plots with no debris (reference plots) or with crimson clover, subterranean clover, rye, or wheat debris. Cover crops were either desiccated by glyphosate or mowed and tilled into the soil. Debris levels, soil temperature, moisture, pH, nitrate, total phenolic acid and compaction were monitored during May to August in both 1992 and 1993. Seedling emergence for all three weed species ranged from <1 to 16% of seeds sown. Surface debris treatments delayed weed seedling emergence compared with the reference plots. Rye and wheat debris consistently suppressed weed emergence; in contrast, the effects of clover debris on weed emergence ranged from suppression to stimulation. Gfyphosate application resulted in a longer delay and greater suppression of seedling emergence in May than in April. In 1993, plots in which living biomass was tilled into the soil were also included and monitored. Weed seedling emergence was stimulated when living biomass was incorporated into the soil. Covariate, correlation and principle component analyses did not identify significant relationships between weed seedling emergence and soil physical and chemical characteristics (e.g., total phenolic acid, nitrate, moisture, temperature). We hypothesize that the observed initial delay of the weed seedling emergence for all three species was likely due to low initial soil moisture. The subsequent rapid recovery of seedling emergence of morning-glory and pigweed but notprickfy sida in the clover compared with the small grain debris plots was likely due to variation in soil allelopathic agents or nitrate-N levels. The stimulation of weed seedling emergence when living biomass was incorporated into the soil was likely caused by an increase in “safe” germination sites coupled with the absence of a zone of inhibition resulting from tillage.