Thirteen-day-old common cocklebur (Xanthium pensylvanicum Wallr. # XANPE) plants were treated with 15 μg glyphosate [N-(phosphonomethyl)glycine] applied to the lowermost true leaves. Growth was inhibited rapidly following treatment. About 56% of applied 14C-glyphosate was taken up within 8 h. Within 4 days after treatment, diffusive resistance increased in treated leaves but did not change in untreated leaves. Glyphosate had little effect on nonstructural carbohydrate content of leaves, but soluble carbohydrate levels of stems and roots had increased by 130 and 180%, respectively, by 56 h after treatment. The uptake of 33P into roots was unaffected by the herbicide, but transport of P to the aerial tissues was severely inhibited. Effects such as inhibition of P movement could result from inhibition of protein synthesis.