Gestures can play a facilitative role in the interpretation of structural ambiguities (Guellaiï, Langus, & Nespor, 2014; Prieto, Borràs-Comes, Tubau, & Espinal, 2013; Tubau, González-Fuente, Prieto, & Espinal, 2015) and are associated with spoken expression of negation (Calbris, 2011; Harrison, 2014a; Kendon, 2002, 2004). This study examines gestural forms and timing patterns with specific interpretations intended by speakers in a context of negation in English where the presence of quantification (all/most/many) yields scope ambiguities, for example, All the students didn’t go = (1). Some number of the students went, but all is not the correct number (negation takes wide scope over the quantifier; not>all), versus (2) some number of the students didn’t go, and all is that number (negation takes narrow scope over the quantifier, all>not; see Horn, 2001, Jackendoff, 1972; Syrett, Simon, & Nisula, 2014b). Twenty-five native English speakers produced scopally ambiguous sentences. Analyses of 317 co-occurring gestures revealed a preponderance of head gestures and use of semantically congruent head shakes, alignment of gestures with the negator, and lengthening of gesture strokes where interpretations involved narrow-scope negation. Results are discussed with reference to scope of negation and gesture (Harrison, 2010, 2013, 2014a, 2014b) particularly in comparison to variable patterns found for prosody (Syrett, Simon, & Nisula, 2014a).