Poloxamer 407 (P407) is a biocompatible thermo-setting polymer, while agarose is a biocompatible thermo-softening material. It is interesting to mix them to examine any possible synergy in thermomechanical properties. In this study, rotational rheometer was adopted to study rheological properties of the mixtures of agarose/P407 gels with different concentrations at various frequencies, strain rates and temperatures. It has revealed that the addition of P407 decreased the gel stiffness by an order of magnitude. For the given combinations in this study, the increase in agarose concentration would increase both the storage modulus and loss modulus of the gel mixtures. The variation in P407 concentration (2.5%-10%) minimally changes the composite moduli. These agarose/P407 gel mixtures also exhibited shear thinning behavior. However, the addition of P407 (2.5%-10%) to agarose gel only has very small effect on thermomechanical properties of agarose gels. The overall transition temperature for these gel mixtures is governed by P407 melting point where the phase change starts around 55°C and the gels completely collapse at the melting temperature of agarose.