A national need is to prepare for and respond to accidental or intentional disasters categorized as chemical, biological, radiological, nuclear, or explosive (CBRNE). These incidents require specific subject-matter expertise, yet have commonalities. We identify 7 core elements comprising CBRNE science that require integration for effective preparedness planning and public health and medical response and recovery. These core elements are (1) basic and clinical sciences, (2) modeling and systems management, (3) planning, (4) response and incident management, (5) recovery and resilience, (6) lessons learned, and (7) continuous improvement. A key feature is the ability of relevant subject matter experts to integrate information into response operations. We propose the CBRNE medical operations science support expert as a professional who (1) understands that CBRNE incidents require an integrated systems approach, (2) understands the key functions and contributions of CBRNE science practitioners, (3) helps direct strategic and tactical CBRNE planning and responses through first-hand experience, and (4) provides advice to senior decision-makers managing response activities. Recognition of both CBRNE science as a distinct competency and the establishment of the CBRNE medical operations science support expert informs the public of the enormous progress made, broadcasts opportunities for new talent, and enhances the sophistication and analytic expertise of senior managers planning for and responding to CBRNE incidents.

This paper discusses a pulse electroplating method for preparing copper (Cu)-coated gas diffusion electrodes (GDEs) for the electrochemical conversion of carbon dioxide (CO2) to hydrocarbons such as ethylene. Ionomer coating and air-plasma surface pre-treatments were explored as means of hydrophilizing the carbon surface to enable adhesion of electrodeposited material. The pulsed-current electrodeposition method used successfully generated copper and copper oxide micro- and nano-particles on the prepared surfaces. Copper(I) species identified on the ionomer-treated GDEs are presumed to be highly active for the selective generation of ethylene as compared to other gaseous byproducts of CO2 reduction. Conversely, copper catalysts deposited onto plasma-treated GDEs were found to have poor activity for hydrocarbon production, likely due to substantial metallic character. Of note, plasma treatment of an ionomer-treated GDE after copper plating yielded further improvements in catalytic activity and durability towards ethylene production.

The Laurentian Great Lakes represent the world's largest freshwater ecosystem and contain irreplaceable biodiversity. Lakewide Action and Management Plans (LAMPs) hold the highest potential for ecosystem management in the Great Lakes but have not specifically addressed biodiversity status or strategies for conservation. For four Great Lakes, recently completed biodiversity conservation strategies (blueprints) have assessed the status and threats to biodiversity and recommended strategies for conservation and restoration; a blueprint is under way also for Lake Superior. Here, we compare the completed blueprints and explore challenges to conservation planning for large ecosystems. We also assess whether earlier blueprints are being adopted and offer suggestions for more effective implementation. All of the blueprints focus on biodiversity in the lakes and coastal areas, and some include tributaries and migratory species. Biodiversity status was rated as fair (out of desirable range but restorable) in each lake, with some exceptions and considerable spatial variability. Aquatic invasive species ranked as a top threat to biodiversity in all four blueprints. Other highly ranked threats included incompatible development, climate change, terrestrial invasive species, dams and barriers, and non-point-source pollutants. The recommended strategies are characterized by six themes: coastal conservation, invasive species, connectivity and hydrology, fish restoration, nearshore water quality, and climate change. Each blueprint highlights high-priority strategies, but successful protection and restoration of Great Lakes biodiversity require revisiting these priorities in an adaptive approach.

Environmental Practice 15:462–480 (2013)

Pictures are a quintessential source of aesthetic pleasure. This makes it easy to forget that they are epistemically valuable no less than they are aesthetically so. Pictures are representations. As such, they may furnish us with knowledge of the objects they represent. In this article I provide an account of why photographs are of greater epistemic utility than handmade pictures. To do so, I use a novel approach: I seek to illuminate the epistemic utility of photographs by situating both photographs and handmade pictures among the sources of knowledge. This method yields an account of photography's epistemic utility that better connects the issue with related issues in epistemology and is relatively superior to other accounts. Moreover, it answers a foundational issue in the epistemology of pictorial representation: ‘What kinds of knowledge do pictures furnish?’ I argue that photographs have greater epistemic utility than handmade pictures because photographs are sources of perceptual knowledge, while handmade pictures are sources of testimonial knowledge.

The main goal of the Working Group was to gather astrometric observations made during the triennum as well as old observations not yet published in the data base. The WG encouraged the making of new observations. A Spring School was organized in China in order to teach the observational techniques of natural satellites to students and young astronomers. New theoretical models of the motion of the satellites and fit of the current models to new observations were used in order to make ephemerides of all the planetary satellites with tools useful for observations such as configurations. These ephemerides named MULTISAT are available at <www.imcce.fr/sat> or at <lnfm1.sai.msu.ru/neb/nss/nssephme.htm>.

Excavations by MoLAS at 30 Gresham Street and 12 Arthur Street in the City of London in 2001 have led to a complete reassessment of the provision of Roman London’s water supply. Four massive first- and second-century wells contained remains of two types of mechanical water-lifting machines or bucket-chain systems. The discoveries allowed identification of a similar bucket-chain from the Cheapside Roman baths, found 50 years earlier by Ivor Noël Hume of the Guildhall Museum. Archaeological and engineering analysis has revealed the water-lifting capacity of the machines, which could have supplied a significant proportion of the town’s population with clean water.

In vitro studies using highly purified calf thymus RNA polymerase II and a fragment spanning the first intron of H3.3 as template DNA have demonstrated the existence of a strong transcription termination site consisting of thymidine stretches. In this study, nuclear run-on experiments have been performed to assess the extent to which transcription elongation is blocked in vivo using DNA probes corresponding to regions 5′ and 3′ of the in vitro termination sites. These studies suggest that H3.3 expression is stimulated following the inhibition of DNA synthesis through the elimination of the transcription elongation block. Interestingly, both the in vivo and in vitro experiments have revealed that the transcriptional block/termination sites are positioned immediately downstream of a 73 bp region that has been over 90% conserved between the chicken and human H3.3 genes. The extreme conservation of this intronic region suggests a possible role in maintaining cis-acting function. Electrophoretic mobility shift experiments show that HeLa cell nuclear extracts contain protein factors that bind specifically to the region of transcription elongation block. Furthermore, we demonstrate a correlation between the protein binding activity and the transcriptional block in cells that have been either arrested at the initiation of S phase or were replication-interrupted by hydroxyurea. DNA footprinting experiments indicate that the region of protein binding is at the 3′ end of the conserved region and overlaps with one of the three in-vitro-mapped termination sites.