Rhynchosporium secalis causes an economically important foliar disease of barley, rye, and other grasses known as leaf blotch or scald. This species has been difficult to classify due to a paucity of morphological features; the genus Rhynchosporium produces conidia from vegetative hyphae directly, without conidiophores or other structures. Furthermore, no teleomorph has been associated with R. secalis, so essentially nothing is known about its phylogenetic relationships. To identify other fungi that might be related to R. secalis, the 18S ribosomal RNA gene and the internal transcribed spacer (ITS) region (ITS1, 5.8S rRNA gene, and ITS2) were sequenced and compared to those in databases. Among 31 18S sequences downloaded from GenBank, the closest relatives to R. secalis were two species of Graphium (hyphomycetes) and two other accessions that were not identified to genus or species. Therefore, 18S sequences were not useful for elucidating the phylogenetic relationships of R. secalis. However, analyses of 76 ITS sequences revealed very close relationships among R. secalis and species of the discomycete genera Tapesia and Pyrenopeziza, as well as several anamorphic fungi including soybean and Adzuki-bean isolates of Phialophora gregata. These species all clustered together with 100% bootstrap support. On the basis of these results, the teleomorph of R. secalis, if it exists, most likely will be a small apothecium produced directly on dead, infected host tissue. The ITS analysis also indicated that higher-level classifications within the discomycetes need to be revised, and that Tapesia and Pyrenopeziza probably do not belong in the Dermateaceae.