Bicolor fluorescent micro-pattern in the polymer film was prepared through the use of a new group of photobase generator containing phthalimido carbamate groups. The photobase generation from phthalimide carbamates was studied from the product analysis of a model compound. The result indicates that amine was produced from the photolytic cleavage of the CN bond of the phthalimide carbamate group. Bicolor fluorescent micropatterns were obtained by treating a copolymer film containing phthalimido groups, which was irradiated with 254 nm UV light through photomask, with fluorescamine and rhodamine, consecutively. Various colored fluorescent micropatterns – green, red, or red-yellow, were obtained on a single polymer film by varying the excitation wavelength.