The actin cytoskeleton is involved in various cellular functions including cell shape determination, organelle transport, and cell polarity establishment. In order to study the function and dynamics of actin in plant cells, methods to image its orientation have to be optimized. Although, green fluorescent proteins (GFP) from the jellyfish Aequoria victoria have been used for visualizing actin filaments in plant cells, the technique is still in its infancy and has some limitations. Actin in plant cells is difficult to preserve and hence visualize mainly because of the problems associated with specimen fixation and processing. This is particularly true for bulky specimens such as plant organs because of the additional requirement for embedding and sectioning after the tissues are fixed and dehydrated. In order to overcome these difficulties, we have modified a method previously used for onion epidermal cells and suspension cells, and applied this to fresh Vibratome sectioned root tissues.