The ITS region of the rRNA genes was amplified using DNA extracted from Terfezia pfeilii fruit-bodies. Two types of restriction
fragment profiles were revealed among 11 fruit-bodies by the Hinf I restriction enzyme. An additional four truffles collected from the
same field one year later were found to have a mixed profile combining both types. Germinated spores from one of these mixed-profile individuals had a single profile belonging to one or the other of the two types. Roots collected around and underneath one
Terfezia fruit-body were traced to different plants. Roots of a watermelon plant (Citrullus vulgaris) showed a non-vesicular-arbuscular
endomycorrhizal association. Restricted ITS amplification fragments of mycorrhized roots from C. vulgaris were shown to match
T. pfeilii fruit-body ITS-RFLP fragments. The significance of this finding with regard to T. pfeilii potential host range is discussed.