Online ordering is currently unavailable due to technical issues. We apologise for any delays responding to customers while we resolve this. For further updates please visit our website: https://www.cambridge.org/news-and-insights/technical-incident
We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.
To save content items to your account,
please confirm that you agree to abide by our usage policies.
If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account.
Find out more about saving content to .
To save content items to your Kindle, first ensure coreplatform@cambridge.org
is added to your Approved Personal Document E-mail List under your Personal Document Settings
on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part
of your Kindle email address below.
Find out more about saving to your Kindle.
Note you can select to save to either the @free.kindle.com or @kindle.com variations.
‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi.
‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
Cytoplasts from single spermatocytes of NZB/BinJ mice were
separated from the nuclei and individually microinjected into B6D2F1 (C57BL/6 × DNBA/2J)
hybrid embryos at the pronuclear stage (20 h after hCG injection). Of 363 zygotes injected, 311 (86%)
survived and developed. From these experiments, we transferred 222 embryos into 20 pseudopregnant
recipients. Eighteen (90%) became pregnant and 82 pups were born (37% of transfers). Mitochondrial DNA
(mt DNA) from the NZB/BinJ strain lacks a RsaI restriction site and can thus be distinguished from
the host embryo following PCR amplification. We were unable to detect the transferred mtDNA in
blastocysts on day 4–5 after injection. Nor could we detect NZB/BinJ mtDNA in placentae, nor
in tissues from mice born to host mothers following the transfer of blastocysts that developed from
injected zygotes. Rejection of paternal mitochondria by the embryo normally occurs at the 4- to 8-cell
stage in mice and is apparently dependent on mutual recognition between the mitochondria and the
nuclear genome. We conclude that this mechanism has probably already developed by the time the germ
cells have become committed to meiosis.
Recommend this
Email your librarian or administrator to recommend adding this to your organisation's collection.