Book contents
- Frontmatter
- Contents
- Foreword
- Preface to the first edition
- Postscript
- Preface to the second edition
- Preface to the third edition
- List of contributors
- Introduction
- 1 Classification and nomenclature
- 2 Culture media: constituents and sterilization
- 3 Principles of isolation
- 4 Bacterial characters and characterization
- 5 Theory and practice of bacterial identification
- 6 Characters of Gram-positive bacteria
- 7 Characters of Gram-negative bacteria
- 8 Taxonomy in theory and practice
- 9 Bacterial identification by cards
- 10 Bacterial identification by computer
- 11 Quality control in microbiology
- Appendices
- A Preparation and control of culture media
- B Staining: reagents and methods
- C Characterization tests
- D Test organisms
- E Preparation and use of Identicards
- F Use of computers
- G The Bacteriological Code
- H The Approved Lists of Bacterial Names
- I Reconciliation of Approaches to Bacterial Systematics
- J Glossary
- References
- Index
D - Test organisms
Published online by Cambridge University Press: 15 December 2009
- Frontmatter
- Contents
- Foreword
- Preface to the first edition
- Postscript
- Preface to the second edition
- Preface to the third edition
- List of contributors
- Introduction
- 1 Classification and nomenclature
- 2 Culture media: constituents and sterilization
- 3 Principles of isolation
- 4 Bacterial characters and characterization
- 5 Theory and practice of bacterial identification
- 6 Characters of Gram-positive bacteria
- 7 Characters of Gram-negative bacteria
- 8 Taxonomy in theory and practice
- 9 Bacterial identification by cards
- 10 Bacterial identification by computer
- 11 Quality control in microbiology
- Appendices
- A Preparation and control of culture media
- B Staining: reagents and methods
- C Characterization tests
- D Test organisms
- E Preparation and use of Identicards
- F Use of computers
- G The Bacteriological Code
- H The Approved Lists of Bacterial Names
- I Reconciliation of Approaches to Bacterial Systematics
- J Glossary
- References
- Index
Summary
Most of the biochemical characterization tests described in this Manual should be controlled by organisms that are known to give either positive or negative reactions under appropriate conditions. For this purpose, we list in Table Dl the species recommended together with the accession numbers of suitable strains available from the National Collection of Type Cultures, Central Public Health Laboratory, Colindale Avenue, London NW9 5HT and, in many instances, also from the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Maryland 20852, USA. As controls for the common tests shown in Table D2, the set of four strains given is adequate. For other bacterial characters, the test strains shown in Table D3 may be used. We emphasize, however, that most of the organisms shown in these tables are pathogenic so that due regard must be paid to safety and hygiene in the laboratory.
For the maintenance and storage of test-control strains, freeze-dried (lyophilized) cultures which retain their characters and keep virtually indefinitely are ideal. However, for those who wish to keep frequently used strains for immediate use, we give in Table D4 suggestions for the nutrient media, cultural conditions and short-term storage period in order to be reasonably sure of their survival before they need replacement from stock cultures.
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- Publisher: Cambridge University PressPrint publication year: 1993