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Additions to the genus Cliostomum (Ramalinaceae) from Australia

Published online by Cambridge University Press:  28 February 2024

Gintaras Kantvilas*
Affiliation:
Tasmanian Herbarium, Tasmanian Museum and Art Gallery, UTAS LPO, Sandy Bay, Tasmania 7005, Australia
*
Corresponding author: Gintaras Kantvilas; Email: Gintaras.Kantvilas@tmag.tas.gov.au

Abstract

Seven species of Cliostomum Fr. recorded from Australia are treated. These include the widespread C. griffithii (Sm.) Coppins, the austral C. praepallidum (Müll. Arg.) Kantvilas & Fryday and the Tasmanian endemic C. vezdae Kantvilas, a reinstated name previously subsumed under C. flavidulum Hafellner & Kalb. Three species are described as new: C. latisporum Kantvilas, a corticolous species containing atranorin and gangaleoidin from coastal Tasmania and New South Wales, characterized by pale pink, soon immarginate apothecia, 0.3–0.9 mm wide, non-capitate, unpigmented paraphyses, and relatively wide, 1(–3)-septate ascospores, 9–15 × 4–6 μm; C. litorale Kantvilas, a saxicolous species containing atranorin and confluentic acid, recorded only from Tasmania, with relatively large, usually pale pinkish apothecia, 0.6–1.5 mm wide, non-capitate paraphyses, and 1(–2)-septate ascospores, 9–13 × 4−6 μm; and C. saxatile Kantvilas, a saxicolous species containing atranorin and lecanoric acid, also known only from Tasmania, with dark brown to black apothecia, 0.3–0.5 mm wide, pigmented with Laurocerasi-brown, capitate paraphyses, and 1-septate ascospores, 7–14 × 3–5 μm. The widespread, coastal Australasian species Tylothallia verrucosa (Müll. Arg.) Kantvilas is transferred to Cliostomum, with the additional synonym Catillaria brisbanensis Räsänen. Megalaria variegata (Müll. Arg.) D. J. Galloway, based on a New Zealand type, is a further synonym of Cliostomum griffithii. All species are illustrated and described on the basis of Australian and Tasmanian specimens.

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Introduction

Although described by Fries (Reference Fries1825), the genus Cliostomum was not generally taken up until it was reinstated by Hawksworth et al. (Reference Hawksworth, James and Coppins1980). Subsequent studies, including those of Gowan (Reference Gowan1990: North America), Kalb & Hafellner (Reference Kalb and Hafellner1992: Madeira), Tønsberg (Reference Tønsberg1992: Norway) and Kantvilas & Elix (Reference Kantvilas and Elix1995: Australia), gradually added more species. In his overview of the genus, by then comprising seven species, Ekman (Reference Ekman1997) speculated that further taxa of Cliostomum remained to be found, either lurking within large genera such as Bacidia, Biatora or Catillaria, or in poorly studied habitats or regions. This certainly proved to be the case, with species from both hemispheres being added by Hawksworth et al. (Reference Hawksworth, Earland-Bennet and Coppins2006), Kantvilas & Fryday (Reference Kantvilas and Fryday2010), Wirth & Kalb (Reference Wirth and Kalb2011), Fryday & Coppins (Reference Fryday and Coppins2012), Fryday & Øvstedal (Reference Fryday and Øvstedal2012), Aptroot (Reference Aptroot2014), Tønsberg & Goward (Reference Tønsberg and Goward2016), Holien & Tønsberg (Reference Holien and Tønsberg2017), van den Boom et al. (Reference van den Boom, Sipman, Divakar and Ertz2017) and Fryday (Reference Fryday2019). Today the genus comprises c. 20 species, of which three are recognized for Australia by McCarthy (Reference McCarthy2023). Molecular investigations involving Cliostomum have been few (e.g. Spjut et al. Reference Spjut, Simon, Guissard, Magain and Sérusiaux2020) and offer little insight into the relationships between individual species.

For many years, the author has noted several additional but undetermined taxa in Australia. After an extensive search for existing overlooked names in other genera, comparative study of Cliostomum species and specimens in many herbaria, and much fieldwork, mostly in Tasmania, these taxa are described here as new to science, and are discussed in the context of a general review of the genus in Australia.

Outline of the genus

Delimiting Cliostomum can be somewhat complex. Ekman (Reference Ekman1997) reviewed in considerable detail its characterization at generic rank, discussing such critical features as the photobiont, apothecial morphology and anatomy, in particular the presence of crystals in the epithecium and proper exciple, the asci and paraphyses, ascospores, pycnidia, conidia and secondary chemistry. Based on morphological and anatomical examination undertaken in the present study, Cliostomum can be characterized by: an ecorticate, crustose thallus; a unicellular, green photobiont with globose cells 6−16 μm diam.; usually pinkish to pale grey, biatorine apothecia with a cupulate proper exciple and epithecium both densely inspersed with minute crystals that mostly dissolve in K; simple to sparsely branched, generally ±straight, often capitate paraphyses; clavate, 8-spored asci approximating the Biatora-type, with an amyloid wall, well-developed amyloid tholus, usually with a slightly more intensely amyloid band adjacent to a conical masse axiale, and a poorly developed or absent ocular chamber (illustrated by Hafellner (Reference Hafellner1984) and Kantvilas & Elix (Reference Kantvilas and Elix1995)); hyaline, non-halonate, (0–)1(–3)-septate ascospores; a secondary chemistry that often includes atranorin and/or usnic acid, together with fatty acids or depsidones.

The closest genus to Cliostomum is Tylothallia, which shares with Cliostomum similar, Biatora-type asci and usually 1-septate ascospores, and also has apothecia where the exciple and epithecium are inspersed with crystals. The close relationship between these two genera has been noted by Ekman (Reference Ekman1997) and Cannon et al. (Reference Cannon, Ekman, Kistenich, LaGreca, Printzen, Timdal, Aptroot, Coppins, Fletcher and Sanderson2021) and clearly deserves further exploration. These authors refer to the more branched and anastomosed paraphyses and immersed pycnidia of Tylothallia as distinguishing features. Certainly the paraphyses of the type species, T. biformigera (Leight.) P. James & H. Kilias, are somewhat thinner (1–1.5 μm), flexuose and distinctly branched and anastomosing towards the apices in comparison to those of Cliostomum species. However, the Australasian species T. verrucosa (Müll. Arg.) Kantvilas has simple to sparingly branched paraphyses (Kantvilas Reference Kantvilas2014). In the past, this species, with its saxicolous thallus containing atranorin with the depsidones gangaleoidin or lecideoidin, and its black apothecia with greenish pigments, meant it was unlikely to be confused for a Cliostomum. However, in recent times (Fryday & Coppins Reference Fryday and Coppins2012; Fryday & Øvstedal Reference Fryday and Øvstedal2012; Fryday Reference Fryday2019), as well as in the present paper, Cliostomum has come to include several saxicolous species with black apothecia. Furthermore, gangaleoidin is now also found in one species (C. latisporum Kantvilas, described below) whose inclusion in Cliostomum is unequivocal. On this basis, Tylothallia verrucosa is transferred to Cliostomum (see below).

Material and Methods

The study is based on specimens housed in the Tasmanian Herbarium (HO), collected mainly by the author, and on the holdings of the Australian National Herbarium (CANB) and the National Herbarium of Victoria (MEL). Comparative data for non-Australian species were drawn mostly from the literature and from exsiccata housed in the herbaria cited above. Anatomical observations and measurements are based on thin, hand-cut sections of the thallus, apothecia and pycnidia, mounted in water, 10% KOH (K), Lugol's iodine, ammoniacal erythrosin and/or lactophenol cotton blue. Ascospore measurements are presented in the format 5th percentile–average–95th percentile, with outlying values in brackets and n signifying the number of observations. Chemical analyses were undertaken mostly by thin-layer chromatography (TLC) using standard methods (Orange et al. Reference Orange, James and White2010); solvent A was the preferred routine medium. The presence of gangaleoidin was confirmed by Dr E. Lacey, Sydney, using high-performance liquid chromatography (Feige et al. Reference Feige, Lumbsch, Huneck and Elix1993).

The more widespread species, notably C. griffithii (Sm.) Coppins and C. verrucosum (Müll. Arg.) Kantvilas, have extensive synonymies. Only new synonyms or those that have been explicitly recorded for Australia are listed in this paper.

Key to Cliostomum in Australia

  1. 1 Thallus sorediate……… 2

    Thallus not sorediate………. 3

  2. 2(1) Thallus with a yellowish tinge, P+ red (containing fumarprotocetraric acid and atranorin); soredia continuous and dominating the thallus……… . C. vezdae

    Thallus whitish grey, P− (containing atranorin and roccellic acid); soredia patchy, spreading irregularly, or in discrete soralia………. C. griffithii

  3. 3(1) Thallus exclusively saxicolous………4

    Thallus corticolous, lignicolous or on dead leaves, very rarely saxicolous………. 6

  4. 4(3) Thallus C+ pink (containing lecanoric acid in addition to atranorin); a species of the hinterland………. C. saxatile

    Thallus C− (lecanoric acid lacking); ±confined to the coast………. 5

  5. 5(4) Apothecia black; thallus containing gangaleoidin or lecideoidin in addition to atranorin………. . C. verrucosum

    Apothecia pale pinkish; thallus containing confluentic acid plus atranorin………. C. litorale

  6. 6(3) Ascospores polarilocular; septum thickened and pierced by a narrow channel………C. praepallidum

    Ascospores (0–)1(–3) septate, with septum simple and unthickened………. 7

  7. 7(6) Thallus containing atranorin and roccellic acid; apothecia usually persistently marginate, pinkish, grey or blackened with Laurocerasi-brown pigment, sometimes piebald; ascospores 3–4.5 μm wide………. C. griffithii

    Thallus containing atranorin and gangaleoidin; apothecia soon immarginate, persistently pinkish and lacking dark pigments; ascospores 4−6 μm wide………C. latisporum

The Australian Species

Cliostomum griffithii (Sm.) Coppins

In D. L. Hawksworth, Lichenologist 12, 106 (1980).—Lichen griffithii Sm., in J. E. Smith & J. E. Sowerby, Engl. Bot. 25, plate 1735 (1807).—Catillaria griffithii (Sm.) H. Magn., Svensk Bot. Tidskr. 13, 80 (1919).

Cliostomum spermogoniatum (Zahlbr.) Kantvilas & Elix, Biblioth. Lichenol. 58, 204 (1995).—Lecidea spermogoniata Zahlbr., Denkschr. Kaiserl. Akad. Wiss. Wien, Math.-Naturwiss. Kl. 104, 310 (1941); type: New Zealand, Wellington, Halcombe near Palmerston North, on Podocarpus totara in forest, H. H. Allan ZA 3340 (CHR347059—lectotype!, fide Galloway (Reference Galloway1985); BM—isolectotype!).

Catillaria banksiae (Müll. Arg.) Zahlbr., Cat. Lich. Univ. 4, 11 (1926).—Patellaria banksiae Müll. Arg., Bull. Herb. Boissier 1, 47 (1893); type: [Australia, Victoria], corticola, in Banksia serrata prope Cheltenham, F. R. M. Wilson n 632 (G—lectotype!, fide Kantvilas & Fryday (Reference Kantvilas and Fryday2010)).

Megalaria variegata (Müll. Arg.) D. J. Galloway, N. Z. J Bot. 42, 116 (2004).—Patellaria variegata Müll. Arg., Bot. J. Linn. Soc. 32, 205 (1896).—Catillaria variegata (Müll. Arg.) Zahlbr., Cat. Lich. Univers. 4, 84 (1926); type: New Zealand, W. Colenso 1578 (BM—lectotype!, fide Galloway (Reference Galloway1985)).

(Fig. 1A–D)

Figure 1. Morphological variation in Cliostomum griffithii. A, coastal form with a smooth thallus and dark-pigmented apothecia. B, swamp woodland form with a ±smooth to scurfy thallus and apothecia with a pale margin and pale to piebald disc. C, ‘old forest’ form, with a scurfy thallus and piebald apothecia. D, alpine form from leaves of Richea pandanifolia, with a distinctly sorediate thallus and dark-pigmented apothecia. Scales = 1 mm. Images: Jean Jarman. In colour online.

Thallus rimose areolate, greyish white to cream-white, ±smooth and to 100–200 μm thick, or rather unevenly granular, verruculose or scurfy, sometimes sorediate, typically forming irregular, diffuse, rather discontinuous, widely spreading patches; soredia farinose to granular, white or greenish when fresh, irregularly patchy or, rarely, in discrete soralia; medulla densely inspersed with minute crystals that partially dissolve in K.

Apothecia 0.3–0.8(–1) mm wide, roundish, superficial, basally constricted; disc pale pink, greyish or blackened, sometimes piebald, occasionally lightly grey-pruinose, plane to undulate to convex, sometimes markedly so and becoming dimpled; proper exciple concolorous with the disc, or pinkish brown and paler, or darker and sometimes black and glossy, usually persistent, a little higher than the disc, entire or, rarely, becoming flexuose or lobed, in section 30–60 μm thick laterally, hyaline to pale yellowish brown within, sometimes brownish, K+ purple-brown at the upper, outer edges, composed of radiating, short-celled, branched hyphae 2–3 μm wide, densely inspersed with minute crystals that dissolve in K. Hypothecium 40–80 μm thick, hyaline, not inspersed. Hymenium 45–70 μm thick, hyaline, overlain by a brownish, K+ purple-brown epithecium composed of the pigmented apices of the paraphyses and inspersed with minute, pale yellow-brown crystals that dissolve fleetingly yellowish in K. Paraphyses 1.5–2 μm wide, markedly capitate, with the apices 3.5–5 μm wide, hyaline to brownish. Asci 30–40 × 11–15 μm. Ascospores ellipsoid-oblong, (0–)1(–2)-septate, 8–11.2–15(–16) × 3–3.5–4(–4.5) μm (n = 70), with a very thin, often indistinct wall and septum.

Pycnidia emergent, black, usually very abundant, 0.1–0.3 mm wide; wall c. 25 μm thick in section, brown, K+ purple-brown; conidia ellipsoid, 3–4.5 × 1.5–2 μm.

Chemistry

Atranorin and roccellic acid (major compounds); thallus weakly K+ yellowish, KC−, C−, P−. The thinness of the thallus and low concentration of these compounds can sometimes render TLC analyses ambiguous.

Remarks

This essentially pan-temperate/cosmopolitan species has an extensive synonymy (see Ekman Reference Ekman1997; Galloway Reference Galloway2007). Only synonyms based on an Australian type or which have been explicitly recorded for Australia are listed above, with the exception of Megalaria variegata, a further synonym based on a type from New Zealand. Additional descriptive data can be found in Galloway (Reference Galloway1985), Gowan (Reference Gowan1990), Kantvilas & Elix (Reference Kantvilas and Elix1995), Ekman (Reference Ekman1997) and Cannon et al. (Reference Cannon, Ekman, Kistenich, LaGreca, Printzen, Timdal, Aptroot, Coppins, Fletcher and Sanderson2021).

Cliostomum griffithii is a highly variable species, best characterized within the genus by the often scurfy thallus containing atranorin and roccellic acid, the black, usually numerous pycnidia, and the small, relatively narrow, mostly 1-septate ascospores. The characteristic pigment found in the apothecia and walls of the pycnidia is Laurocerasi-brown, which gives a purplish brown reaction in K (Meyer & Printzen Reference Meyer and Printzen2000; Kantvilas & Coppins Reference Kantvilas and Coppins2019). In occasional specimens, a trace of additional Fucatus-violet, yielding a vivid K+ turquoise reaction (Kantvilas Reference Kantvilas2009), was also present in the pycnidia. Pigments are absent or barely present in pale-coloured apothecia, but intense and present in the epithecium and/or exciple of darker ones. Pigmented apothecia have markedly capitate paraphyses; in paler apothecia, the swollen apices are less pronounced but are nevertheless observable. Specimens with essentially unpigmented apothecia could be confused with C. latisporum, but usually, at least in the case of larger thalli, there will be some apothecia that show hints of dark pigmentation. Furthermore, whereas the apothecial margin in C. latisporum tends to become excluded as the apothecia age and become increasingly convex, in C. griffithii the margin tends to be a little higher than the disc and persistent into maturity. The two species can also be distinguished by their ascospores (4–6 μm wide in C. latisporum). The chemical difference between the two, although helpful, can be difficult to confirm due to the frequently low concentration of substances.

Also a little similar to C. griffithii is C. praepallidum (Müll. Arg.) Kantvilas & Fryday, but this species is readily distinguished by its larger, polarilocular ascospores (Kantvilas & Fryday Reference Kantvilas and Fryday2010). The saxicolous C. litorale and C. saxatile Kantvilas differ from C. griffithii chemically as well as morphologically (q.v.). In the Tasmanian and Australian biota in general, C. griffithii could also be confused with several other species, including as yet not fully determined species of Lecania and Biatora s. lat., which are known to occur in similar habitats. In such cases, the presence of crystals in the exciple and epithecium, characteristic of the genus as a whole, is a helpful identifying character, as is the combination of capitate paraphyses, ascospore size and thallus chemistry. One species, ascribed tentatively to Lecania chlorotiza (Nyl.) P. James s. lat., differs further by containing gyrophoric acid and having Bacidia-type asci. Two additional taxa, C. corrugatum (Ach.) Fr. and C. piceicola Holien & Tønsberg, share some features with C. griffithii but differ in thallus characters, lack roccellic acid and contain usnic acid in their apothecia (Ekman Reference Ekman1997; Holien & Tønsberg Reference Holien and Tønsberg2017). Both are confined to the Northern Hemisphere.

The morphological variation of the thallus and apothecia displayed by C. griffithii is remarkable (see Fig. 1), but a detailed examination of a wide range of specimens, both from Tasmania and elsewhere, failed to reveal consistent differences that could suggest that several cryptic species might be involved. The thallus ranges from rather thick, smooth and continuous, to scurfy and granular, to patchy with scattered soralia. Apothecia can be unpigmented and pale, or entirely black, or piebald, or various combinations of a dark disc with a pale exciple, or a dark exciple with a pale disc. However, lurking within what is regarded here as C. griffithii is one entity that may well represent an undescribed taxon, but which requires considerable further study. It grows on the dead leaves of Richea pandanifolia, together with C. griffithii and C. vezdae Kantvilas & Elix, but differs by containing usnic acid in the thallus in addition to atranorin and roccellic acid, atranorin only in the apothecia, having discrete, roundish soralia 0.3–0.7 mm wide, at least when young, and somewhat smaller ascospores, 7.5–11 × 2.5–3.5 μm.

Distribution and ecology

This species has a very wide global distribution and has been recorded from most temperate regions of the world (Ekman Reference Ekman1997). It is chiefly corticolous or lignicolous, but is also rarely found on rocks.

In Tasmania, C. griffithii displays a remarkably broad distribution, occurring abundantly in at least four very distinct and widely spatially and ecologically separate vegetation formations. In cool temperate rainforest, it occurs commonly on the dry, flaky bark of Nothofagus cunninghamii within the rich association of lichens that predominates on dry, sheltered aspects of the largest, oldest trees. In analogous forests in New South Wales, it is found on N. moorei. Associated species in this habitat include calicioid lichens, Lecanactis abietina (Ach.) Körb. and L. mollis (Stirt.) Frisch & Ertz, Arthonia apteropteridis Kantvilas & Vězda, Chrysothrix xanthina (Vain.) Kalb and species of Micarea. This dry, sheltered corticolous habitat in ancient climax forests is typical for Cliostomum griffithii in other parts of the world (Ekman Reference Ekman1997; Cannon et al. Reference Cannon, Ekman, Kistenich, LaGreca, Printzen, Timdal, Aptroot, Coppins, Fletcher and Sanderson2021). At subalpine elevations, C. griffithii occurs in woodlands and tall heathlands, frequently in communities dominated by the ancient conifer Athrotaxis cupressoides. Here it is one of the dominant lichens found on the dead, dry leaves of the arborescent heath Richea pandanifolia. This lichen community is also very species-rich and whilst it shares many species with that of the dry, ancient trunk, forest community, it also supports several additional species characteristic of this unusual habitat; for example, Chiodecton flavovirens G. Thor, Clistomum vezdae, Lecanactis latispora Egea & Torrente and Opegrapha cf. atra Pers. In coastal scrub and heathland, Cliostomum griffithii can be common on the twigs and trunks of small trees and shrubs such as Banksia marginata, Myoporum insulare and Leucopogon parviflorus, where it can be associated with Austroparmelina pseudorelicina (Jatta) A. Crespo et al., Flavoparmelia rutidota (Hook.f. & Taylor) Hale, Halegrapha mucronata (Stirt.) Lücking, Lecanora flavopallida Stirt. and Ramalina inflata (Hook.f. & Taylor) Hook.f. & Taylor. Finally, Cliostomum griffithii is often the dominant epiphytic species in swampy, lowland Melaleuca ericifolia-dominated woodland, forming extensive thalli that cover entire trunks of papery bark for several metres. These woodlands are considered to be of very high conservation value, especially for their lichens (Baker et al. Reference Baker, Grove, de Salas, Byrne, Cave, Bonham, Moore, Cooke and Kantvilas2021; de Salas et al. Reference de Salas, Baker, Cave and Kantvilas2023), and C. griffithii is associated with numerous lichen species that are considered rare and highly localized, for example Bactrospora metabola (Nyl.) Egea & Torrente, B. paludicola Kantvilas, Bacidia septosior (Nyl.) Zahlbr., Coniocarpon cinnabarinum DC., Enterographa micrographa (Nyl.) Redinger, Haematomma sorediatum R. W. Rogers and Pseudocyphellaria aurata (Ach.) Vain. Vastly different though these vegetation types are, they are all essentially ‘old growth’ communities with an extended history of ecological stability and continuity. One Tasmanian collection is from sheltered conglomerate outcrops in high elevation buttongrass (Gymnoschoenus) moorland, a habitat not unusual for other chiefly corticolous, wet forest species.

Selected specimens examined

Australia: Tasmania: Mt Rufus Track, 42°07ʹS, 146°05ʹE, 1120 m, 1972, G. C. Bratt 72/1042 (HO); Mt Field National Park, W shore of Lake Dobson, 42°41ʹS, 146°35ʹE, 1040 m, 1981, L. Tibell 11097 (distributed as Lich. Sel. Upsalienses 265) (CANB); Robbins Island Track, N of Denium Hill, 40°44ʹS, 144°53ʹE, 2 m, 1993, J. A. Elix 40273 & G. Kantvilas 149/93 (CANB, HO, TU); Bruny Island, Cape Queen Elizabeth, 43°15ʹS, 147°26ʹE, 100 m, 2007, G. Kantvilas 431/07 (HO); Cuvier Valley Track, 42°01ʹS, 146°05ʹE, 790 m, 2011, G. Kantvilas 158/11 (HO); Cape Portland, Little Musselroe River, 40°47ʹS, 148°04ʹE, 5 m, 2018, G. Kantvilas 154/18 (HO); North East Ridge Track to Mt Anne, 42°54ʹS, 146°24ʹE, 340 m, 2022, G. Kantvilas 320/22 (HO); Scotts Peak Road, c. 1.1 km S of Frodshams Pass, 42°49ʹS, 146°23ʹE, 550 m, 2023, G. Kantvilas 247/23 (HO) (sorediate specimen with additional usnic acid). South Australia, Kangaroo Island: Brown Beach, 35°48ʹS, 137°50ʹE, 10 m, 2012, G. Kantvilas 425/12 & B. de Villiers (AD, HO); ibid., c. 1 km SW of Ballast Head, 35°46ʹS, 137°48ʹE, 3 m, 2013, G. Kantvilas 349/13 & B. de Villiers (AD, HO). Victoria: S of Lake Gillear, 38°26ʹS, 142°36ʹE, 1987, W. H. Ewers 667 (CANB); Bemm River, 37°37ʹ30ʺS, 148°53ʹ12ʺE, 65 m, 2008, G. Kantvilas 86/08 & J. Elix (HO); Furnell Landing, 37°43ʹ20ʺS, 149°08ʹ14ʺE, 1 m, 2008, G. Kantvilas 153/08 & J. Elix (HO, MEL); Walkerville Coastal Reserve, 38°51ʹ22ʺS, 145°59ʹ50ʺE, 2009, V. Stajsic 5142b (HO, MEL). New South Wales: Mt William, Barrington Tops NP, 32°04ʹS, 151°28ʹE, 1400 m, 1988, G. Kantvilas 312/88 (CANB, HO, NSW).

Cliostomum latisporum Kantvilas sp. nov.

MycoBank No.: MB 850546

Similar to Cliostomum griffithii but with the apothecia persistently pale pink, lacking dark pigments and at length becoming immarginate, non-capitate, unpigmented paraphyses, somewhat wider ascospores, 9−15 × 4−6 μm, and containing atranorin and gangaleoidin as the major secondary compounds.

Type: Australia, New South Wales, Burrewarra Point, 1 km W of Tomakin, 35°51ʹS, 150°11ʹE, 20 m, on dead shrub in coastal woodland, 16 September 1993, J. A. Elix 30193 (CANB—holotype; HO—isotype).

(Fig. 2A)

Figure 2. Morphology of Australian Cliostomum species. A, C. latisporum (note the unpigmented, immarginate apothecia). B, C. litorale. C, C. praepallidum. D, C. verrucosum. Scales = 1 mm. In colour online.

Thallus smooth to patchily rimose to verruculose, greyish white to cream-white, occasionally highly reduced to ±absent, to 50–200 μm thick, esorediate, forming irregular, diffuse patches to c. 2 cm across; medulla inspersed with minute crystals that dissolve in K.

Apothecia 0.3–0.9 mm wide, roundish, superficial, basally constricted; disc whitish to pale pink, epruinose, at first plane, becoming undulate and then convex with age; proper exciple concolorous with the disc or a little paler, entire, becoming inconspicuous to excluded in older, more convex apothecia, in section 40–55 μm thick laterally, pale yellowish brown, composed of radiating, branched and anastomosing hyphae 1.5–2 μm wide with occasional oil vacuoles to 3 μm wide, densely inspersed with minute crystals that dissolve in K and scattered, larger K-insoluble crystals. Hypothecium 25–80 μm thick, hyaline, not inspersed. Hymenium 40–60 μm thick, hyaline, overlain by pale yellowish brown crystals that dissolve in K. Paraphyses 1.5–2 μm wide, with the apices not markedly capitate, hyaline, gradually expanding to 2−3(–4) μm wide. Asci 30–45 × 10–16 μm. Ascospores ellipsoid, 1(–3)-septate, (9–)10–11.8–14(–15) × 4–4.8–5.5(–6) μm (n = 90), with a thin, distinct wall and septum c. 0.3 μm thick.

Pycnidia uncommon, emergent, black and speck-like, 0.07–0.13 mm wide; wall c. 20–40 μm thick in section, containing greenish, N+ crimson pigment; conidia globose, 2.5–3 μm diam.

Chemistry

Atranorin and gangaleoidin, sometimes with traces of fatty acids; thallus weakly K+ yellowish, KC−, C−, P−. The thinness of the thallus and low concentration of these compounds can render TLC analyses ambiguous. Traces of usnic acid can occur in the apothecia.

Etymology

The specific epithet alludes to the relatively broad ascospores of the new species when compared to the somewhat morphologically similar C. griffithii.

Remarks

This species is superficially similar to C. griffithii and, for many years, specimens now recognized as C. latisporum were subsumed under that name. However, extensive field observations and detailed anatomical and chemical examination support its recognition as a distinct taxon. Thallus morphology is very similar to that of coastal forms of C. griffithii with which it grows, but whereas that species can become scurfy and/or sorediate, the thallus of C. latisporum is invariably esorediate and, at most, verruculose. The apothecia of the two species are also similar, but again, whereas those of C. griffithii are frequently dark pigmented or piebald, those of the new species are persistently pale pinkish. Furthermore, those of C. griffithii tend to remain marginate, but in C. latisporum the margin becomes ±excluded or at least reduced and inconspicuous as the apothecia age and become increasingly convex. Anatomical differences between the two species are more clear-cut. The paraphyses of C. latisporum are not capitate, but widen gradually and are never pigmented, whereas those of C. griffithii are markedly capitate. Finally, the ascospores of the new species, although overlapping somewhat in dimensions, are consistently wider, with a very distinct wall and septum. The chemical difference between the two species is distinct but can be difficult to establish on account of the thin, small thalli and low concentration of the substances. Some of the specimens cited below were too scant for analysis. Gangaleoidin is an uncommon metabolite but it also occurs in C. verrucosum, a saxicolous, coastal species with black apothecia. It is observed on developed TLC plates as a pale yellow spot, a little lower than atranorin.

Pycnidia were found only in the type collection and differ from those of C. griffithii in the pigment of the wall (aeruginose, N+ crimson, rather than brown, K+ purple-brown) and in the shape of the conidia. The distinction is stark but I am reluctant to overemphasize it until further specimens with pycnidia are found.

Distribution and ecology

The new species is widely scattered along the northern and eastern coast of Tasmania and extends to the southern coast of New South Wales on the Australian mainland. Most collections are from coastal heathland and scrub, where it colonizes the twigs of small shrubs such as Acacia sophorae and Leucopogon parviflorus. Associated lichens include Arthothelium endoaurantiacum Makhija & Patw., Caloplaca maccarthyi S. Y. Kondr. et al., Cliostomum griffithii, Lecanora flavopallida Stirt. and species of Amandinea and Buellia. It has also been found in Melaleuca ericifolia-dominated swampy coastal woodland and, in New South Wales, in mangrove vegetation where it grew on the twigs of Avicennia.

Specimens examined

Australia: Tasmania: Rocky Cape, Burgess Cove, 40°52ʹS, 145°30ʹE, sea level, 1999, G. Kantvilas 296/99 (HO); end of Robbins Island Track, 40°45ʹS, 144°53ʹE, sea level, 1999, G. Kantvilas 262/99 (HO); Cape Portland, Musselroe Bay Conservation Area, ‘Abalone Rocks’, 40°47ʹ26ʺS, 148°06ʹ08ʺE, 3 m, 2018, G. Kantvilas 389/18 (HO); King Island, Lavinia State Reserve, Nine Mile Beach, 39°42′12ʺS 144°06'17ʺE, 20 m, 2023, G. Kantvilas 360/23 (HO). New South Wales: Cullendulla Creek Nature Reserve, 3 km NE of Batemans Bay, 32°42ʹ04ʺS, 150°11ʹ56ʺE, 1 m, 2008, J. A. Elix 45654 (CANB).

Cliostomum litorale Kantvilas sp. nov.

MycoBank No.: MB 850547

Unique to the genus Cliostomum in containing confluentic acid in addition to atranorin, and further characterized by the esorediate thallus, the relatively large, usually pale pinkish apothecia, 0.6–1.5 mm wide, the non-capitate paraphyses, and the 1(–2)-septate ascospores, 9–13 × 4–6 μm.

Type: Australia, Tasmania, Frogmore Peninsula, Midway Point, 42°47ʹS, 147°31ʹE, 2 m, on littoral sandstone, 28 June 2017, G. Kantvilas 132/17 (HO—holotype).

(Fig. 2B)

Thallus rimose areolate to rather granular, pale yellowish grey to beige-grey, sometimes abraded and then whitish, forming irregular, diffuse patches to c. 10 cm wide; individual areoles and granules 0.15–1.5 mm wide and to 0.3–0.6 mm thick, irregular in outline, plane to markedly convex, contiguous and fused together or rather dispersed and separated by deep cracks; prothallus usually lacking, occasionally effuse, bluish grey and visible at the periphery of the thallus; medulla white, densely inspersed with minute crystals that partially dissolve in K.

Apothecia 0.6–1.5 mm wide, roundish, superficial, basally constricted; disc usually pale pink, epruinose, occasionally becoming discoloured smoky blue-grey, rarely blackened, plane to undulate to convex, sometimes markedly so; proper exciple concolorous with and a little higher than the disc, persistent, entire to flexuose, sometimes quite deeply lobed, in section 50–80 μm thick laterally, hyaline to pale yellowish brown, composed of radiating, short-celled, branched hyphae c. 2 μm wide, with frequent oil vacuoles to 5 μm wide, densely inspersed with minute crystals that dissolve in K as well as clusters of insoluble crystals. Hypothecium (40–)70–100 μm thick, hyaline, not inspersed. Hymenium 45–65 μm thick, hyaline, overlain and inspersed in the upper part with minute crystals that dissolve fleetingly yellowish in K, in darkened apothecia with a bluish green, N+ crimson pigment. Paraphyses (1.5–)2 μm wide, with the apices unpigmented, not capitate, sometimes slightly enlarged to 2.5 μm wide. Asci (35–)40–50 × 12–18 μm. Ascospores ellipsoid, 1(–2)-septate, 9–10.6–13 × 4–4.9–6 μm (n = 75), when mature with a distinct wall c. 0.3 μm thick.

Pycnidia soon emergent, black, 0.25–0.4 mm wide, with the wall 30–40 μm thick in section, greenish black, intensifying greenish in K, N+ crimson; conidia oblong-ellipsoid, 4–5 × 1.5–2 μm.

Chemistry

Atranorin and confluentic acid; thallus weakly K+ yellowish, KC−, C−, P−.

Etymology

The specific epithet alludes to the coastal distribution of the new species.

Remarks

The combination of a rather coarse, areolate to granular thallus, relatively large, pale pink, biatorine apothecia, Biatora-type asci and 1-septate, hyaline ascospores, together with the saxicolous, coastal habitat render this species very easily recognizable, and there are no known, potentially confusing species in the temperate Australian biota. Amongst the known species of Cliostomum, the presence of confluentic acid is unique. Although most species of Cliostomum are corticolous or lignicolous, there are several saxicolous taxa. The sorediate C. subtenerum Coppins & Fryday and the esorediate C. tenerum (Nyl.) Coppins & Ekman both contain atranorin, stictic acid and zeorin (Ekman Reference Ekman1997; Fryday & Coppins Reference Fryday and Coppins2012). The chemistry of C. albidum Fryday, from the Falkland Islands, has not been analyzed but the reported P+ orange-red reaction (Fryday Reference Fryday2019) indicates that it is different from the new species. Also from the Falkland Islands, C. falklandicum Fryday & Coppins contains atranorin only (Fryday & Øvstedal Reference Fryday and Øvstedal2012), whereas C. longisporum Fryday is distinguished best by its ascospores that are 15–23 μm long (Fryday Reference Fryday2019). Finally, C. namibicum V. Wirth & Kalb differs by its long conidia (9–12 × 0.8–1.2 μm) and by containing usnic acid (Wirth & Kalb Reference Wirth and Kalb2011). Australian saxicolous taxa include C. saxatile, which differs by its small, black apothecia and by the presence of lecanoric acid. A further saxicolous species, C. verrucosum, which also occurs in littoral habitats, has black apothecia with greenish, N+ crimson pigments and contains gangaleoidin or lecideoidin in addition to atranorin.

All collections of the new species are very uniform in morphology and anatomy. Pycnidia are relatively uncommon, and are most frequent in thalli where apothecia are few. Ascospores are almost invariably 1-septate, and a second septum is at best developed near the spore apex and then only weakly.

Distribution and ecology

This species is known only from south-eastern Tasmania where almost all records are from sheltered underhangs on coastal, coarse-grained Tertiary sandstone. The littoral zone in Tasmania is typically very lichen-rich with respect to both species diversity and biomass. Yet the microhabitat colonized by the new species tends to be remarkably devoid of lichens, supporting, at most, depauperate thalli of the common taxa which dominate more exposed sites, for example, Buellia spp., Caloplaca kilcundaensis S. Y. Kondr. & Kärnefelt, Lecanora dispersa (Pers.) Sommerf. and Cliostomum verrucosum.

Specimens examined

Australia: Tasmania: type locality, 2017, G. Kantvilas 127/17 (HO); Spring Bay Bill, ‘Cresponea Cliffs’, 42°33ʹS, 147°56ʹE, 5 m, 2021, G. Kantvilas 12/21 (HO); Randalls Bay, south-western headland, 43°15ʹS, 147°07ʹE, 3 m, 2021, G. Kantvilas 185/21 (HO); Alum Cliffs, Kingston, 42°58ʹS, 147°20ʹE, 35 m, 2022, G. Kantvilas 330/22 (HO).

Cliostomum praepallidum (Müll. Arg.) Kantvilas & Fryday

Lichenologist 42, 542 (2010).—Patellaria praepallida Müll. Arg., Miss. Sc. Cap Horn, Lich., 166 (1888).

(Fig. 2C)

Thallus effuse, smooth, continuous or patchy, sometimes very thin and inapparent, pale grey, to cream-grey, 30–80 μm thick, forming diffuse patches rarely more than c. 20 mm wide; medulla not inspersed.

Apothecia 0.3–1 mm diam., roundish, superficial, basally constricted; disc pale pink to pale orange, rather waxy and glossy, epruinose, plane at first, becoming convex with age, at length sometimes multi-divided and with clusters of regenerating apothecia; proper exciple concolorous with the disc or a little paler, slightly inrolled when young, usually becoming excluded with age, in section 40–90(–120) μm thick laterally, hyaline to pale yellowish brown, composed of radiating, short-celled, branched and anastomosing, highly gelatinized hyphae 1–1.5 μm wide, densely inspersed with minute crystals that dissolve in K. Hypothecium 50–130 μm thick, hyaline to pale yellowish, not inspersed. Hymenium 60–70 μm thick, hyaline, overlain and inspersed in the upper part by minute, pale yellow-brown crystals that dissolve fleetingly yellowish in K. Paraphyses 1.5–2 μm wide, with the apices hyaline, to 2–5 μm wide. Asci 35–50 × 10–18 μm. Ascospores ellipsoid, (11–)12–14.3–18 × (5–)6–7.3–9 μm (n = 120), invariably 1-septate, polarilocular, with the septum 3–5 μm thick and the narrow, connecting channel clearly evident.

Pycnidia not observed.

Chemistry

Atranorin, roccellic acid and norcaperatic acid (major compounds); thallus weakly K+ yellowish, KC−, C−, P−.

Remarks

A detailed discussion of this species is provided by Kantvilas & Fryday (Reference Kantvilas and Fryday2010). Its relatively large, polarilocular ascospores, a feature shared with the Scottish, epiphytic C. coppinsii Fryday & Kantvilas, suggest that it occupies a rather peripheral position within the genus. However, for the present, chemical and anatomical characters relating to the apothecium and the ascus make Cliostomum the ‘genus of best fit’. Cliostomum praepallidum is relatively easily distinguished in the Tasmanian biota, although confirmation of its identity invariably requires anatomical examination of apothecial sections in order to observe the diagnostic crystals, asci and ascospores. Although superficially similar to C. griffithii, which is chemically identical, its apothecia are never dark pigmented and remain orange-pink throughout development. Furthermore, these two species are not known to co-occur.

Distribution and ecology

In Tasmania, this species occurs on twigs and young branches in cool temperate rainforest, wet eucalypt forest, scrub and wet heathland, where it is part of a rich assemblage of chiefly crustose lichens (see Kantvilas & Fryday Reference Kantvilas and Fryday2010). It is also known from southern South America.

Selected specimens examined

Australia: Tasmania: Lake Esperance, 43°14ʹS, 146°46ʹE, 980 m, 1963, P. James (BM, HO); Mt Sprent, 42°47ʹS, 145°58ʹE, 850 m, 1987, G. Kantvilas 132/87A (E, HO); Bluff River Gorge, 42°33ʹS, 147°41ʹE, 200 m, 2017, G. Kantvilas 372/17 (HO); Mt Wedge, 42°51ʹS, 146°18ʹE, 1145 m, 2107, G. Kantvilas 156/17 (HO); Lake Skinner Track, 42°57ʹS, 146°41ʹE, 910 m, 2021, G. Kantvilas 136/21 (HO).

Cliostomum saxatile Kantvilas sp. nov.

MycoBank No.: MB 850548

A saxicolous species distinguished from all others in the genus by containing lecanoric acid in addition to atranorin, and further characterized by the esorediate thallus, dark brown to black apothecia, 0.3–0.5 mm wide and pigmented with Laurocerasi-brown, the capitate paraphyses, and 1-septate ascospores, 7–14 × 3–5 μm.

Type: Australia, Tasmania, The Gnomon, 41°11ʹS, 146°02ʹE, 475 m, on vertical, sheltered rock faces in wet sclerophyll forest, 25 May 1991, G. Kantvilas 237/91 (HO—holotype; M—isotype).

(Fig. 3B)

Figure 3. Morphology of Australian Cliostomum species. A, C. vezdae, showing the sorediate thallus, yellowish pink apothecia and tiny, black pycnidia. B, C. saxatile, with black apothecia and black, emergent pycnidia. Scales = 1 mm. Images: Jean Jarman. In colour online.

Thallus granular areolate, whitish, forming diffuse, wide-spreading patches; individual areoles to 0.3 mm wide and to c. 0.2 mm thick, irregular in outline, plane to convex, sometimes a little gnarled, contiguous or rather dispersed over a very thin, wispy, whitish prothallus; medulla white, inspersed with minute crystals that dissolve in K.

Apothecia 0.3–0.5 mm wide, round, superficial, basally constricted; disc dark brown to black, epruinose, plane, rarely becoming convex; proper exciple concolorous with and ±level with the disc, rarely a little paler, persistent, entire, in section 40–60 μm thick laterally, hyaline to pale yellowish brown within, dark purple-brown, K+ intensifying at the upper, outer edge, composed of radiating, short-celled, branched hyphae 2–2.5 μm wide, with occasional oil vacuoles to 4 μm wide and with the outermost, pigmented cells to 5 μm wide, inspersed with minute crystals that dissolve in K, sometimes also with clusters of insoluble crystals. Hypothecium 70–80 μm thick, hyaline, K+ faint yellowish, not inspersed. Hymenium 40–50 μm thick, hyaline, overlain by a purple-brown, K+ intensifying epithecium 5−10 μm thick and inspersed in the upper part with minute crystals that dissolve in K. Paraphyses 1.5–2 μm wide, with the apices markedly capitate, 4–6.5 μm wide, externally purple-brown. Asci 32–42 × 10–14 μm. Ascospores ellipsoid to fusiform, thin-walled, (0–)1-septate, (7–)7.5–9.4−12(–14) × 3–3.7–4(–5) μm (n = 75).

Pycnidia emergent, black, 0.2–0.4 mm wide, with the wall often rather gnarled, 30–60 μm thick, in section purple-brown, intensifying purplish in K; conidia oblong-ellipsoid, 3.5–5 × 1.5–2 μm.

Chemistry

Atranorin and lecanoric acid; thallus weakly K+ yellowish, KC+ fleeting pink, C+ fleeting pink, P−.

Etymology

The specific epithet refers to the habitat of the new species.

Remarks

Comparative diagnostic features of the other saxicolous species of Cliostomum are discussed under C. litorale (see above) and are not repeated here. Superficially, with its small Laurocerasi-brown pigmented apothecia and capitate paraphyses, C. saxatile most closely resembles the chiefly corticolous C. griffithii, but the presence of lecanoric acid is unique and has never been reported previously for the genus. In addition, the ascospores of C. saxatile are marginally shorter than those of C. griffithii.

Distribution and ecology

The new species is known only from the type collection, from vertical, deeply sheltered crevices of a large crag of highly siliceous, Ordovician conglomerate in wet sclerophyll forest. It has not been sighted since, despite more than three decades of lichen investigations in Tasmania. Its occurrence in a highly sheltered microhabitat is not unusual for species of Cliostomum.

Cliostomum verrucosum (Müll. Arg.) Kantvilas comb. nov.

MycoBank No.: MB 850549

Patellaria verrucosa Müll. Arg., Bull. Herb. Boissier 4, 94 (1896).—Catillaria verrucosa (Müll. Arg.) Zahlbr., Cat. Lich. Univ. 4, 84 (1926).—Tylothallia verrucosa (Müll. Arg.) Kantvilas, Australas. Lichenol. 74, 13 (2014); type: Victoria, Sandringham, on maritime rock at high water mark, 1893, Rev. F. R. M. Wilson 1743 (G—holotype!).

Catillaria rimosa (Müll. Arg.) Zahlbr., Cat. Lich. Univers. 4, 69 (1926).—Patellaria rimosa Müll. Arg., Bull. Herb. Boissier 1, 48 (1893) nom. illegit; type: Victoria, Lorne, on sandstone, 1892, Rev. F. R. M. Wilson 1402 (G—holotype!).

Tylothallia pahiensis (Zahlbr.) Hertel & H. Kilias, in H. Hertel, Mitt. Bot. Staatssamml. München 19, 446 (1983).—Lecidea pahiensis Zahlbr., Akad. Wiss. Wien, Math.-Naturwiss. Kl., Denkschr. 104, 303 (1941); type: New Zealand, South I., Southland, Foveaux Strait, Pahia Point, on granitic coastal rocks, viii.1935, J. S. Thomson T2242 (W—holotype; BM—isotype!); for location of additional isotypes, see Galloway (Reference Galloway2007).

Catillaria brisbanensis Räsänen, Arch. Soc. Zool. Bot. Fenn. Vanamo 3, 182 (1949); type: Australia, South Queensland, Brisbane, Hellend, ad saxa silicata, 1890, F. R. M. Wilson (H—holotype!).

(Fig. 2D)

Thallus rimose-areolate to verruculose, sometimes rather lumpy, esorediate, whitish grey to pale greenish grey, rarely rather pale brownish grey, to 1.5 mm thick, forming extensive irregular patches; prothallus marginal, black to bluish black; medulla white, inspersed with minute crystals that partially dissolve in K.

Apothecia 0.4–1.2(–1.7) mm wide, roundish, superficial, basally constricted; disc black, plane to undulate, sometimes becoming convex, epruinose or occasionally lightly greyish pruinose; proper exciple concolorous with the disc or pale greyish or brownish at the outer edge, persistent except in the oldest, most convex apothecia, in section 40–110 μm thick laterally, bluish green at the outer edge, hyaline within, densely inspersed with clusters of large and small crystals, the former rod-shaped, c. 2–3 μm long, pale brown and insoluble in K, the latter hyaline, minute and soon dissolving in K. Hypothecium 60–120 μm thick, sometimes becoming massive, hyaline, not inspersed. Hymenium 50–65 μm thick, not inspersed, hyaline or with a faint yellowish or pinkish tinge, overlain by a greenish black, K−, N+ crimson epithecial layer. Paraphyses 12 μm thick, with apices 3–4 μm wide, coated with blue-green pigment. Asci 40–50 × 11–17 μm. Ascospores ellipsoid, (0–)1-septate, (8–)8.5–10.3–12.5(–16) × 4–4.9–5.5(–6) μm (n = 60).

Pycnidia abundant, visible as black specks; wall 10-20 µm thick in section, brown, unchanged in K, sometimes with additional greenish, N+ crimson pigment at the ostiole; conidia 3–3.5 × 1.5–2 μm.

Chemistry

Atranorin, ±either lecideoidin or gangaleoidin; thallus weakly K+ yellowish, KC−, C−, P−.

Remarks

The transfer of this common species from Tylothallia to Cliostomum is discussed above in the introductory paragraphs. All its salient features, notably the presence of crystals in the exciple, the Biatora-type asci, simple, capitate paraphyses and 1-septate hyaline ascospores, support its inclusion there. The status of the remaining species of Tylothallia (T. biformigera) awaits further study although this taxon has branched and anastomosing paraphyses. In the field and in the absence of anatomical examination, C. verrucosum could be confused with Lecanora austrooceanica Hertel & Leuckert (hyaline, simple ascospores) and species of Amandinea (brown, 1-septate ascospores) which occur in the same habitat, although these lichens tend to be considerably less common and have a duller grey thallus. Additional descriptive data are provided by Galloway (Reference Galloway1985, Reference Galloway2007) and Kantvilas (Reference Kantvilas2014).

This species has an extensive synonymy to which is now added Catillaria brisbanensis. Annotations on this type specimen (in H) by Harald Kilias (in 1983) indicate that he already recognized this species as belonging to the genus Tylothallia.

Distribution and ecology

This species is locally abundant and widespread on siliceous, coastal rocks, ranging from Tasmania, along the southern Australian coast and northwards to at least south-eastern Queensland; it is also known from New Zealand. This conspicuous species is the major contributor to the white coloration of littoral lichen mosaics, contrasting sharply with the yellowish or orange Caloplaca species, yellow Lecanora subcoarctata (C. Knight) Hertel and blackish or dark brown Catillaria austrolittoralis Kantvilas & van den Boom, Rinodina blastidiata Matzer & H. Mayrhofer and Verrucaria species with which it grows.

Selected specimens examined

Australia: Tasmania: Don Heads, 41°10ʹS, 146°20ʹE, 1963, G. C. Bratt 978 & J. A. Cashin (HO); Penguin, 1968, W. A. Weber & D. McVean L-49696 (COLO, HO); Sleepy Bay, 42°08ʹS, 148°19ʹE, sea level, 1984, G. Kantvilas 129/84 & P. James (BM, HO); Swansea, 42°07ʹS, 148°04ʹE, 1–5 m, 1996, H. Mayrhofer 13380 (GZU, HO); Lion Rock, 43°36ʹS, 146°49ʹE, 1 m, 2013, G. Kantvilas 35/13 (HO); Flinders Island, The Dock, 39°48ʹS, 147°52ʹE, 1 m, 2014, G. Kantvilas 289/14 (HO); Stony Head Training Area, eastern end of Maitland Bay, 40°59ʹS, 147°01ʹE, 2 m, 2021, G. Kantvilas 176/21 (HO). South Australia, Kangaroo Island: Kona, Antechamber Bay, 35°47ʹS, 138°05ʹE, 2008, G. Kantvilas 334/08 (AD, HO); W of Windmill Bay, 35°51ʹS, 138°07ʹE, 40 m, 2012, G. Kantvilas 504/12 (AD, HO). Victoria: Phillip Island, Barrys Beach, 38°31ʹS, 146°12ʹE, 6 m, 1966, G. C. Bratt 3574 & R. C. Weeks (HO). New South Wales: Baragoot Point, 36°24ʹS, 150°05ʹE, 1978, J. A. Elix 4583 & G. Rambold (HO) (distributed as H. Hertel: Lecideaceae Exsiccatae 140); Boulder Bay, Bournda National Park, 36°44ʹ51ʺS, 149°58ʹ53ʺE, 20 m, 2012, L. H. Cave 1797 (HO).

Cliostomum vezdae Kantvilas & Elix

Biblioth. Lichenol. 58, 208 (1995); type: Australia, Tasmania, Weindorfers Forest, 41°38ʹS, 145°56ʹE, on trunk of Richea pandanifolia in rainforest, 920 m, 1988, G. Kantvilas 30/88 (HO—holotype!; E—isotype!).

(Fig. 3A)

Thallus ±entirely farinose sorediate, greyish white with a faint yellowish tinge, to c. 150 μm thick but usually much thinner, forming irregular, diffuse, thinly dispersed patches to 15 cm or more in width; individual soredia 20–50 μm wide.

Apothecia 0.25–0.6 mm wide, roundish, superficial and nestled amongst the soredia; disc pale yellowish to pinkish grey, rarely discoloured bluish grey, usually slightly pruinose, plane to undulate, becoming convex and at length convoluted; proper exciple concolorous with the disc or a little paler, persistent in young apothecia but becoming excluded in older, convex ones, entire, in section 30–60 μm thick laterally, pale yellowish brown and inspersed with minute crystals that dissolve yellowish in K, later becoming reflexed and excluded, composed of highly gelatinized, radiating, branched hyphae c. 2.5 μm wide. Hypothecium 50–60(–150) μm thick, hyaline, not inspersed. Hymenium 40–60 μm thick, hyaline, inspersed in the upper part and overlain by yellow-brown crystals that dissolve yellow in K; occasionally with patchy blue-green, N+ crimson pigment. Paraphyses 0.8–1.5 μm wide, with the apices unpigmented, rounded or tapered, sometimes a little expanded to 2–2.5 μm wide. Asci 30–55 × 12–18 μm. Ascospores ellipsoid-oblong, almost invariably 1-septate, 8–10.1–12(–14.5) × 3–4.1–5 μm (n = 65), with a distinct wall and septum when mature.

Pycnidia superficial, rare, visible as minute, black specks c. 0.1 mm wide; wall brown, K± purplish brown; conidia fusiform to bacilliform, 4–5 × 1.5–2 μm.

Chemistry

Atranorin and fumarprotocetraric acid (major compounds), sometimes with traces of additional fatty acids (usually caperatic or roccellic acids); thallus weakly K+ yellowish, KC−, C−, P+ orange-red.

Remarks

Ekman (Reference Ekman1997) synonymized Cliostomum vezdae with the widespread Northern Hemisphere species C. flavidulum Hafellner & Kalb, chiefly because of the superficial similarities in their sorediate thalli and their identical secondary chemistry. On further consideration and study, however, the two taxa are better treated as distinct, with C. flavidulum differing by having a continuous, initially areolate thallus, frequently delimited by a whitish to blue-black prothallus, and which is intensely sulphur yellow when fresh, as well as by having immersed, unpigmented pycnidia (Ekman Reference Ekman1997, personal communication 2023). In contrast, C. vezdae is sorediate from the outset, very pale yellowish at all stages of development, lacks a prothallus and has dark-pigmented pycnidia.

There are several other sorediate species of the genus, but none have the same chemical composition as C. vezdae. In the Northern Hemisphere, the corticolous species C. leprosum (Räsänen) Holien & Tønsberg has a whitish grey thallus containing atranorin and caperatic acid (Ekman Reference Ekman1997), C. haematommatis (Keisll.) D. Hawksw. et al. has a pale bluish grey thallus and contains atranorin and an unknown substance (Hawksworth et al. Reference Hawksworth, Earland-Bennet and Coppins2006), C. spribillei Goward & Tønsberg has a yellowish green thallus containing usnic acid (Tønsberg & Goward Reference Tønsberg and Goward2016), and C. subtenerum Coppins & Fryday is saxicolous and contains atranorin, stictic acid and zeorin (Fryday & Coppins Reference Fryday and Coppins2012). In the Falkland Islands, C. violascens (Müll. Arg.) Fryday is sorediate and contains apothecial Melaena-red pigment (Fryday Reference Fryday2019). The widespread C. griffithii, which occurs in Tasmania and is sometimes sympatric with C. flavidulum, can be occasionally and patchily granular sorediate but contains atranorin and roccellic acid in the thallus and Laurocerasi-brown pigment in darkened apothecia.

Distribution and ecology

This species appears to be rare in Tasmania, where it is restricted to cool temperate rainforest, especially at higher elevations. Like several other species of Cliostomum, it favours sheltered, drier microhabitats. The dead retained leaves and inclined trunks of the rosette tree heath Richea pandanifolia appear to offer a preferred microhabitat, where it occurs together with the far more common C. griffithii. Associated lichens in this habitat are listed under that species.

Specimens examined

Australia: Tasmania: Yarlington Tier, 42°32ʹS, 147°18ʹE, 620 m, 1987, G. Kantvilas 90/87 (BM, HO); Anthony Road, 41°50ʹS, 145°38ʹE, 600 m, 1991, G. Kantvilas 448/91 (HO); track to Woolleys Tarn, 42°55ʹS, 146°40ʹE, 950 m, 2004, G. Kantvilas 222/04 (HO); Lake Skinner Track, 42°57ʹS, 146°41ʹE, 850 m, 2020, G. Kantvilas 33/20 (HO); Scotts Peak Road, c. 1.1 km S of Frodshams Pass, 42°49ʹS, 146°23ʹE, 550 m, 2023, G. Kantvilas 246/23 (HO).

Acknowledgements

I am grateful to Stefan Ekman and an anonymous referee whose comments greatly improved this paper. I also thank Ern Lacey for determining the identity of gangaleoidin in C. latisporum, Jack Elix for much helpful discussion on chemical and other matters, and the curators of the National Herbarium of Victoria (MEL) and the Australian National Herbarium (CANB) for their hospitality and loans of specimens. For the photographs I thank Jean Jarman. This study was aided by ABRS grant 4-EHINNOL from the Australian Biological Resources Study.

Author ORCID

Gintaras Kantvilas, 0000-0002-3788-4562.

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Figure 0

Figure 1. Morphological variation in Cliostomum griffithii. A, coastal form with a smooth thallus and dark-pigmented apothecia. B, swamp woodland form with a ±smooth to scurfy thallus and apothecia with a pale margin and pale to piebald disc. C, ‘old forest’ form, with a scurfy thallus and piebald apothecia. D, alpine form from leaves of Richea pandanifolia, with a distinctly sorediate thallus and dark-pigmented apothecia. Scales = 1 mm. Images: Jean Jarman. In colour online.

Figure 1

Figure 2. Morphology of Australian Cliostomum species. A, C. latisporum (note the unpigmented, immarginate apothecia). B, C. litorale. C, C. praepallidum. D, C. verrucosum. Scales = 1 mm. In colour online.

Figure 2

Figure 3. Morphology of Australian Cliostomum species. A, C. vezdae, showing the sorediate thallus, yellowish pink apothecia and tiny, black pycnidia. B, C. saxatile, with black apothecia and black, emergent pycnidia. Scales = 1 mm. Images: Jean Jarman. In colour online.