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Viability, acrosome morphology and fertilizing capacity of boar spermatozoa treated with strontium chloride

Published online by Cambridge University Press:  01 February 2008

Konosuke Okada ,
Chiara Palmieri ,
Leonardo Della Salda  and
Irena Vackova
Konosuke Okada*
Affiliation:
Department of Animal Science, Nippon Veterinary and Life Science University (NVLU), 1-7-1 Kyonan-cho, Musashino, Tokyo 180-0023, Japan. Institute of Animal Production (VUZV), POB 1, CZ-104 01 Prague 10, Czech Republic.
Chiara Palmieri
Affiliation:
Dipartimento di Scienze Biomediche Comparate, Teramo University, Piazza Aldo Moro 45, 64100 Teramo, Italy.
Leonardo Della Salda
Affiliation:
Dipartimento di Scienze Biomediche Comparate, Teramo University, Piazza Aldo Moro 45, 64100 Teramo, Italy.
Irena Vackova
Affiliation:
Institute of Animal Production (VUZV), POB 1, CZ-104 01 Prague 10, Czech Republic.
*
1All correspondence to: Konosuke Okada. Department of Animal Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-0023, Japan. e-mail: okada@nvlu.ac.jp
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Summary

The positive effect of strontium ions (Sr2+) on sperm motility, capacitation and acrosome reaction has been demonstrated in the mouse, human, guinea pig and hamster. In the present study, we have evaluated the effect of Sr2+ on the viability and acrosome morphology of boar spermatozoa, and on the fertilization and development after the microinjection of Sr2+-treated spermatozoa into porcine oocytes. Before incubation, 79% of spermatozoa were classified as propidium iodide (PI)-negative (live) using the LIVE/DEAD Sperm Viability Kit. After incubation with strontium chloride (SrCl2), 39% (0 mM; no divalent cations), 25% (1.9 mM) and 24% (7.5 mM) of them were classified as PI-negative. The proportion of spermatozoa that had initiated the acrosome reaction was higher in Sr2+-containing medium than in Sr2+-free medium, when assessed by electron microscopy. There was no significant difference in percentage of spermatozoa initiating the acrosome reaction between Sr2+-treated groups (1.9 mM: 22%, 7.5 mM: 33%, p > 0.05). After the microinjection of spermatozoa incubated with SrCl2, 67% (1.9 mM) and 61% (7.5 mM) of injected oocytes were successfully fertilized, and then 43% (1.9 mM) and 41% (7.5 mM) contained a fully decondensed sperm head. Sham-injected oocytes were significantly activated at a lower rate than Sr2+-treated groups (27%, p < 0.05). Next, after microinjection of spermatozoa incubated with 1.9 mM SrCl2 (n = 51), 45% of injected oocytes cleaved on day 2, and 18% developed to blastocysts on day 7 (sham-injection, n = 48: 15% to cleavage and 0% to blastocyst). These results demonstrate that Sr2+ is likely to positively affect the fertilizing capacity of spermatozoa in the pig.

Keywords

Acrosome reactionICSIPigStrontiumViability
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 49 - 56
Copyright
Copyright © Cambridge University Press 2008

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