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Reduction in cytoplasmic lipid content in bovine embryos cultured in vitro with linoleic acid in semi-defined medium is correlated with increases in cryotolerance

Published online by Cambridge University Press:  09 September 2015

Mônica F. Accorsi ,
Beatriz Caetano da Silva Leão ,
Nathália Alves de Souza Rocha-Frigoni ,
Silvia Helena Venturoli Perri  and
Gisele Zoccal Mingoti
Mônica F. Accorsi
Affiliation:
School of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP–Universidade Estadual Paulista, Araçatuba, SP 16050–680, Brazil.
Beatriz Caetano da Silva Leão
Affiliation:
School of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP–Universidade Estadual Paulista, Araçatuba, SP 16050–680, Brazil. School of Agrarian and Veterinarian Sciences, Departament of Animal Reproduction, UNESP–Universidade Estadual Paulista, Jaboticabal, SP 16050–680, Brazil.
Nathália Alves de Souza Rocha-Frigoni
Affiliation:
School of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP–Universidade Estadual Paulista, Araçatuba, SP 16050–680, Brazil. School of Agrarian and Veterinarian Sciences, Departament of Animal Reproduction, UNESP–Universidade Estadual Paulista, Jaboticabal, SP 16050–680, Brazil.
Silvia Helena Venturoli Perri
Affiliation:
School of Veterinary Medicine, Laboratory of Reproductive Physiology, UNESP–Universidade Estadual Paulista, Araçatuba, SP 16050–680, Brazil.
Gisele Zoccal Mingoti*
Affiliation:
School of Veterinary Medicine, Department of Animal Health, UNESP–Universidade Estadual Paulista, Araçatuba 16050–680, São Paulo, Brazil. School of Agrarian and Veterinarian Sciences, Departament of Animal Reproduction, UNESP–Universidade Estadual Paulista, Jaboticabal, SP 16050–680, Brazil.
*
All correspondence to: G.Z. Mingoti, School of Veterinary Medicine, Department of Animal Health, UNESP–Universidade Estadual Paulista, Araçatuba 16050–680, São Paulo, Brazil. Tel: +55 18 3636 1375. Fax: +55 18 3636 1352. E-mail: gmingoti@fmva.unesp.br
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Summary

We examined whether culturing embryos with linoleic acid (LA) in semi-defined medium reduces lipid accumulation and improves cryosurvival after vitrification. Embryos were cultured with LA (100 μM) and a semi-defined medium was used during in vitro culture (IVC), in which the fetal calf serum was substituted by bovine serum albumin (BSA). There was a reduction (P < 0.05) in the embryonic development rate (Control: 25.8% versus LA: 18.5%), but the proposed system was effective in promoting the decrease (P = 0.0130) in the intracellular lipid content (Control: 27.3 ± 0.7 versus LA: 24.6 ± 0.7 arbitrary fluorescence units of embryos stained with the fluorescent dye Nile Red), consequently increasing (P = 0.0490) the embryo survival after 24h of culture post-warming (Control: 50.0% versus LA: 71.7%). The results question the criteria used to evaluate the efficiency of an in vitro production system specifically with relation to the maximum number of blastocysts produced and suggest that might be more appropriate to improve the desired characteristics of embryos generated in accordance with the specific purpose of in vitro embryo production, commercial or scientific. In conclusion, supplying LA to serum-free culture medium was found to adversely affect the rates of embryo development to the blastocyst stage, but significantly reduced embryo lipid accumulation and improved cryopreservation survival.

Keywords

Bovine embryoIn Vitro ProductionLinoleic acidLipidVitrification
Type
Research Article
Information
Zygote , Volume 24 , Issue 4 , August 2016 , pp. 485 - 494
Copyright
Copyright © Cambridge University Press 2015 

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