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Multiple aster formation is frequently observed in bovine oocytes retrieved from 1-day stored ovaries

Published online by Cambridge University Press:  03 March 2015

H. Hara ,
M. Tagiri ,
M. Hirabayashi  and
S. Hochi
H. Hara
Affiliation:
Interdisciplinary Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. National Institute for Physiological Sciences, Okazaki, Aichi 444–8787, Japan.
M. Tagiri
Affiliation:
Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
M. Hirabayashi
Affiliation:
National Institute for Physiological Sciences, Okazaki, Aichi 444–8787, Japan.
S. Hochi*
Affiliation:
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Interdisciplinary Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
*
All correspondence to: S. Hochi. Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Tel: +81 268 215350. Fax: +81 268 215830. e-mail: shochi@shinshu-u.ac.jp
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Summary

We have recently reported that multiple aster formation after in vitro fertilization (IVF) was one of the factors that negatively affected the developmental competence of vitrified-warmed bovine matured oocytes, and that short-term culture of the post-warm oocytes with an inhibitor of Rho-associated coiled-coil kinase (ROCK) suppressed the multiple aster formation and improved the blastocyst yield. The present study was conducted to investigate whether increased multiple aster formation following IVF was involved in impaired developmental competence of stored ovary-derived bovine oocytes. Oocytes retrieved from 1-day stored ovaries had lower developmental potential to day 8 blastocysts when compared with those from fresh ovaries (37 versus 63%). Immunostaining of α-tubulin 10 h post-IVF revealed that a higher incidence of multiple aster formation occurred in oocytes retrieved from stored ovaries than from fresh ovaries (31 versus 15%). Treatment of post-in vitro maturated (post-IVM) oocytes with ROCK inhibitor for 2 h significantly suppressed the incidence of multiple aster formation (10 versus 32% in the control group). However, the suppression effect of ROCK inhibitor on multiple aster formation in IVM/IVF oocytes did not improve blastocyst yield from stored ovary-derived oocytes (41 versus 37% in the control group). These results suggested that the higher incidence of multiple aster formation by bovine ovary storage was not responsible for the decreased developmental competence of IVF oocytes.

Keywords

BlastocystsBovine IVFMultiple astersOvary storageROCK inhibitor (Y-27632)
Type
Research Article
Information
Zygote , Volume 24 , Issue 1 , February 2016 , pp. 115 - 120
Copyright
Copyright © Cambridge University Press 2015 

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