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Micromorphological and ultrastructural description of spermatozoa from squirrel monkeys (Saimiri collinsi Osgood, 1916)

Published online by Cambridge University Press:  14 January 2020

Danuza L. Leão ,
Wlaisa V. Sampaio ,
Patrícia C. Sousa ,
Arlindo A.A. Moura Open the ORCID record for Arlindo A.A. Moura [Opens in a new window] ,
Irma C. Oskam ,
Regiane R. Santos Open the ORCID record for Regiane R. Santos [Opens in a new window]  and
Sheyla F.S. Domingues
Danuza L. Leão*
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil Post graduation programme in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém, Pará, Brazil
Wlaisa V. Sampaio
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil Post graduation programme in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém, Pará, Brazil Federal Rural University of the Amazon, Campus Parauapebas, Pará, Brazil
Patrícia C. Sousa
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil
Arlindo A.A. Moura
Affiliation:
Laboratory of Animal Physiology, Federal University of Ceará, Ceará,Brazil
Irma C. Oskam
Affiliation:
The Animal Production Experimental Center, Norwegian University of Life Sciences, Ås, Norway Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Regiane R. Santos
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil
Sheyla F.S. Domingues
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil Post graduation programme in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém, Pará, Brazil The Animal Production Experimental Center, Norwegian University of Life Sciences, Ås, Norway Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
*
Author for correspondence: Danuza Leite Leão. Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, BR 316 Km 61, CEP 68740-970, Castanhal, Pará, Brazil. Tel: +55 91 33114707. E-mail: danleao.88@gmail.com
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Summary

Saimiri collinsi is used as an animal model in biotechnology research for conservation of species from the genus Saimiri. However, the development of biotechnologies depends on a proper knowledge of the sperm morphology to understand the basic aspects of sperm physiology, as potential male fertility depends on different cellular sperm structures. With this purpose, this study characterized the micromorphological and ultrastructural characteristics of squirrel monkeys (Saimiri collinsi) sperm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM electromyography revealed that a normal Saimiri collinsi sperm measures 71.7 ± 0.7 μm with lateral tail insertion, a paddle-shaped flattened head and an acrosome occupying most of the head. TEM also showed that the middle piece is characterized by a central 9 + 2 microtubule axoneme surrounded by nine dense fibres, and that the mitochondria were juxtaposed, forming the mitochondrial sheath. Here we provide the first micromorphological and ultrastructure description of S. collinsi sperm.

Keywords

Electron microscopyNeotropical primatesScanningSpermTransmission
Type
Research Article
Information
Zygote , Volume 28 , Issue 3 , June 2020 , pp. 203 - 207
Copyright
© Cambridge University Press 2020

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References

Almeida, DVC, Lima, JS, Leão, DL, Oliveira, KG, Santos, RR, Domingues, SFS and Miranda, MS (2018) The effects of Trolox on the quality of sperm from captive squirrel monkey during liquefaction in the extender ACP-118™. Zygote 26, 333–35.CrossRefGoogle ScholarPubMed
Arakaki, PR, Carvalho, FM, Castro, PHG, Muniz, JAPC and Valle, RDRD (2017) Collection, evaluation, and coagulum dissolution of semen from Goeldi’s monkey, Callimico goeldii. Folia Primatol 88, 334–43.CrossRefGoogle ScholarPubMed
Arakaki, PR, Nichi, M, Monteiro, FOB, Muniz, JAPC, Guimarães, MABV and Valle, RDRD (2019) Comparison of semen characteristics and sperm cryopreservation in common marmoset (Callithrix jacchus) and black-tufted-ear marmoset (Callithrix penicillata). J Med Primatol 48, 32–42.CrossRefGoogle Scholar
Baccetti, B, Capitani, S, Collodel, G, Di Cairano, G, Gambera, L, Moretti, E and Piomboni, P (2001) Genetic sperm defects and consanguinity. Hum Reprod 16, 1365–71.CrossRefGoogle ScholarPubMed
Bartoov, B, Eltes, F, Weissenberg, R and Lunenfeld, B (1980) Morphological characterization of abnormal human spermatozoa using transmission electron microscopy. Arch Androl 5, 305–22.CrossRefGoogle ScholarPubMed
Bedford, JM and Nicander, L (1971) Ultrastructural changes in the acrosome and sperm membranes during maturation of spermatozoa in the testis and epididymis of the rabbit and monkey. J Anat 108, 527–43.Google ScholarPubMed
Bezerra, LGP, Souza, ALP, Silva, HVR, Vasconcelos, FR, Moura, AAA, Pereira, AF, Oliveira, MF and Silva, AR (2018) Ultrastructural description of fresh and frozen/thawed sperm derived from collared peccaries (Pecari tajacu Linnaeus, 1758). Microscop Res Tech 81, 1–9.CrossRefGoogle Scholar
Bloom, E (1973) The ultrastructure of some characteristic sperm defects and a proposal for a new classification of the bull spermogram. Nord Vet 25, 83–91.Google Scholar
Bornens, M (2012) The centrosome in cells and organisms. Science 335, 422–26.CrossRefGoogle ScholarPubMed
Gomendio, M and Roldan, ER (1991) Sperm competition influences sperm size in mammals. Proc Biol Sci 243, 181–5.Google ScholarPubMed
Gould, KG (1980) Scanning electron microscopy of the primate sperm. Int Ver Cytol 63, 323–55.CrossRefGoogle ScholarPubMed
Gould, KG and Martin, DE (1978) Comparative morphology of primate spermatozoa using scanning electron microscopy. II. Families Cercopithecidae, Lorisidae, Lemuridae. J Hum Evol 7, 637–42.CrossRefGoogle Scholar
Humphries, S, Evans, JP and Simmons, LW (2008) Sperm competition: linking form to function. BMC Evol Biol 8, 319.CrossRefGoogle Scholar
IUCN (2019) The IUCN Red List of Threatened Species. Version 2019-2. http://www.iucnredlist.org. Accessed 20 August 2019.Google Scholar
Leão, DL, Miranda, AS, Brito, AB, Lima, JS, Santos, RR and Domingues, SFS (2015) Efficacious long-term cooling and freezing of Sapajus apella semen in ACP-118®. Anim Reprod Sci 159, 118–23.CrossRefGoogle Scholar
López-Armengol, MF, Jurado, SB, Pelufo, V and Aisen, EG (2012) A quantitative ultramorphological approach for systematic assessment of sperm head regions: an example in rams. Cryobiology 64, 223–34.CrossRefGoogle ScholarPubMed
Martin, DE, Gould, KG and Warner, H (1975) Comparative morphology of primate spermatozoa using scanning electron microscopy. I. Families Hominidae, Pongidae, Cercopithecidae and Cebidae. J Hum Evol 4, 287–92.CrossRefGoogle Scholar
Nakazato, C, Yoshizawa, M, Isobe, K, Kusakabe, KT, Kuraishi, T, Hattori, S, Matsumoto, H, Fukui, E, Kuwahata, A, Ochi, M, Kiso, Y and Kai, C (2015) Morphological characterization of spermatozoa of the night monkey. J Mamm Ova Res 31, 37–40.CrossRefGoogle Scholar
Nussdorfer, P, Cilenšek, I, Zorn, B and Petrovič, D (2018) Adapted methods for scanning electron microscopy (SEM) in assessment of human sperm morphology. Bosn J Basic Med Sci 18, 43–8.Google ScholarPubMed
Oliveira, KG, Leão, DL, Almeida, DVC, Santos, RR and Domingues, SFS (2015) Seminal characteristics and cryopreservation of sperm from the squirrel monkey, Saimiri collinsi. Theriogenology 84, 743–9.CrossRefGoogle ScholarPubMed
Oliveira, KG, Santos, RR, Leão, DL, Brito, AB, Lima, JS, Sampaio, WV and Domingues, SFS (2016a) Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species. Cryobiology 72, 283–9.CrossRefGoogle ScholarPubMed
Oliveira, KG, Santos, RR, Leão, DL, Queiroz, HL, Paim, FP, Vianez-Júnior, JLSG and Domingues, SFS (2016b) Testicular biometry and semen characteristics in captive and wild squirrel monkey species (Saimiri sp.). Theriogenology 86, 879–87.CrossRefGoogle Scholar
Ozkavukcu, S, Erdemli, E, Ayca Isik, A, Oztuna, D and Karahuseyinoglu, S (2008) Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet 25, 403–11.CrossRefGoogle ScholarPubMed
Pesch, S, Bostedt, H, Failing, K and Bergmannc, M (2006) Advanced fertility diagnosis in stallion semen using transmission electron microscopy. Anim Reprod Sci 91, 285–98.CrossRefGoogle ScholarPubMed
Sampaio, WV, Oliveira, KG, Leão, DL, Caldas-Bussiere, MC, Queiroz, HL, Paim, HP, Santos, RR and Domingues, SFS (2017) Morphologic analysis of sperm from two neotropical primate species: comparisons between the squirrel monkeys Saimiri collinsi and Saimiri vanzolinii. Zygote 25, 1–8.CrossRefGoogle ScholarPubMed
Silva, HVR, Nunes, TGP, Ribeiro, LR, Freitas, LA, Oliveira, MF, Assis Neto, AC, Silva, AR and Silva, LDM (2019) Morphology, morphometry, ultrastructure, and mitochondrial activity of jaguar (Panthera onca) sperm. Anim Reprod Sci 203, 84–93.CrossRefGoogle ScholarPubMed
Sousa, PC, Santos, EAA, Bezerra, JAB, Lima, GL, Castelo, TS, Fontenele-Neto, JD, Silva, AR (2013) Morphology, morphometry and ultrastructure of captive six-banded armadillo (Euphractus sexcinctus) sperm. Anim Reprod Sci 140, 279–85.CrossRefGoogle ScholarPubMed
Steinberg, ER, Nieves, M, Ascunce, MS, Palermo, AM and Mudry, MD (2009) Morphological and genetic characterization of Saimiri boliviensis. Int J Primatol 30, 29–41.CrossRefGoogle Scholar
Swanson, WF, Valle, RR, Carvalho, FM, Arakaki, PR, Rodas-Martínez, AZ, Muniz, JAPC and García-Herreros, M (2016) Sperm morphology assessment in captive neotropical primates. Reprod Dom Anim 51, 623–7.CrossRefGoogle ScholarPubMed
Visco, V, Raffa, S, Elia, J, Delfino, M, Imbrogno, N, Torrisi, MR and Mazzilli, F (2010) Morphological sperm defects analyzed by light microscopy and transmission electron microscopy and their correlation with sperm motility. Int J Urol 17, 259–66.CrossRefGoogle ScholarPubMed
WHO (2010) Laboratory Manual for the Examination and Processing of Human Semen, 5th edn. Geneva, Switzerland: WHO Press.Google Scholar
Zamboni, L, Zemjanis, R and Stefanini, M (1971) The fine structure of monkey and human spermatozoa. Anat Rec 169, 129–53.CrossRefGoogle ScholarPubMed