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The localization of LAP2β during pronuclear formation in bovine oocytes after fertilization or activation

Published online by Cambridge University Press:  01 May 2006

Mamiko Isaji ,
Hisataka Iwata ,
Hiroshi Harayama  and
Masashi Miyake
Mamiko Isaji
Affiliation:
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan.
Hisataka Iwata
Affiliation:
Department of Animal Science, Tokyo University of Agriculture, Funako 1737, Atugi, 246-0034, Japan.
Hiroshi Harayama
Affiliation:
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan.
Masashi Miyake*
Affiliation:
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan.
*
All correspondence to: M. Miyake, Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan. e-mail: miyake@kobe-u.ac.jp
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Summary

We have shown that the assembly of lamin-associated polypeptide (LAP) 2β was detected surrounding the chromatin mass around the time of extrusion of the second polar body (PB) in some fertilized oocytes, but not in most activated oocytes, by using A23187 and cycloheximide (CaA + CH). Here, we immunohistologically analysed the correlation between LAP2β assembly and chromatin condensation in fertilized and activated oocytes during the second meiosis. In bovine cumulus cells, the onset of LAP2β assembly was observed around anaphase chromosomes with strongly phosphorylated histone H3. No LAP2β assembled around the chromosomes in the first and second polar bodies and the alternative oocyte chromatin (oCh) if histone H3 was phosphorylated. Only histone H3 of oCh was completely dephosphorylated during the telophase II/G1 transition (Tel II/G1), and then LAP2β assembled around only the oCh without phosphorylated histone H3. In the oocytes activated by CaA + CH, LAP2β did not assemble around the condensed oCh during the Tel II/G1 transition, although their histone H3 dephosphorylation occurred rather rapidly compared with that of the fertilized oocytes. The patterns of histone H3 dephosphorylation and LAP2β assembly in oocytes activated by CaA alone showed greater similarity to those in fertilized oocytes than to those in oocytes activated by CaA + CH. These results show that LAP2β assembles around only oCh after complete dephosphorylation of histone H3 after fertilization and activation using CaA alone, and that the timing of histone H3 dephosphorylation and LAP2β assembly in these oocytes is different from that of somatic cells. The results also indicate that CH treatment inhibits LAP2β assembly around oCh but not histone H3 dephosphorylation.

Keywords

ActivationBovineFertilizationLAP2βOocytes
Type
Research Article
Information
Zygote , Volume 14 , Issue 2 , May 2006 , pp. 157 - 167
Copyright
Copyright © Cambridge University Press 2006

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