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Inhibition of Hsp90 during in vitro maturation under thermoneutral or heat shock conditions compromises the developmental competence of bovine oocytes

Published online by Cambridge University Press:  15 September 2022

Eliza Diniz de Souza ,
Jessica Fernanda da Silva e Souza ,
Pedro Manoel de Oliveira Netto ,
Luciano de Rezende Carvalheira ,
Ribrio Ivan Tavares Pereira Batista Open the ORCID record for Ribrio Ivan Tavares Pereira Batista [Opens in a new window] ,
Carolina Capobiango Romano Quintão ,
Iuri Drumond Louro  and
Luiz Sergio Almeida Camargo Open the ORCID record for Luiz Sergio Almeida Camargo [Opens in a new window]
Eliza Diniz de Souza
Affiliation:
Universidade Federal do Espírito Santo, Av. Mal. Campos, 1468 – Maruipe, Vitória, ES, Brazil. 29047-105
Jessica Fernanda da Silva e Souza
Affiliation:
Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, Juiz de Fora, MG, Brazil. 36036-900
Pedro Manoel de Oliveira Netto
Affiliation:
Embrapa Dairy Cattle, Rua Eugênio do Nascimento, 610, Juiz de Fora, MG, Brazil. 36038-330
Luciano de Rezende Carvalheira
Affiliation:
Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Caixa postal 567, Belo Horizonte, MG, Brazil. 31270-901
Ribrio Ivan Tavares Pereira Batista
Affiliation:
Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niteroi, RJ, Brazil. 24230-340
Carolina Capobiango Romano Quintão
Affiliation:
Embrapa Dairy Cattle, Rua Eugênio do Nascimento, 610, Juiz de Fora, MG, Brazil. 36038-330
Iuri Drumond Louro
Affiliation:
Universidade Federal do Espírito Santo, Av. Mal. Campos, 1468 – Maruipe, Vitória, ES, Brazil. 29047-105
Luiz Sergio Almeida Camargo*
Affiliation:
Embrapa Dairy Cattle, Rua Eugênio do Nascimento, 610, Juiz de Fora, MG, Brazil. 36038-330
*
Author for correspondence: Luiz Sergio Almeida Camargo. Embrapa Dairy Cattle, Rua Eugênio do Nascimento, 610, Dom Bosco CEP: 36038-330, Juiz de Fora, MG, Brasil. E-mails: luiz.camargo@embrapa.br or camargo3112@gmail.com
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Summary

Heat shock protein 90 (Hsp90) is critical for cell homeostasis but its role on bovine oocyte maturation is not well known. We investigated the importance of Hsp90 for competence of bovine oocyte using 17-(allylamino)-17-demethoxygeldanamycin (17AAG), an inhibitor of Hsp90, during in vitro maturation (IVM). Three experiments evaluated the effect of 17AAG on developmental competence of oocytes matured in vitro under thermoneutral (38.5ºC) or heat shock (HS; 41.5ºC) temperatures. The first experiment found that the blastocyst rates were lower (P < 0.05) with 2 µM 17AAG compared with the untreated control (0 µM). The abundance of HSF1 transcripts was higher in oocytes matured with 2 µM than with 0 and 1 µM 17AAG, whereas the abundance of HSP90AA1 and HSPA1A transcripts was lower (P < 0.05) with 1 and 2 µM than with 0 µM. The second experiment found that 2 µM 17AAG for 12 or 24 h during IVM decreased (P < 0.05) the blastocysts rates. In the third experiment, the association of 2 μM 17AAG with HS for 12 h during IVM resulted in lower (P < 0.05) blastocysts rates than 17AAG, HS or untreated control. In conclusion, inhibition of Hsp90 during in vitro maturation compromises further embryo development; the association of Hsp90 inhibition with HS aggravates the deleterious effect of both on oocyte developmental competence.

Keywords

CattleGeldanamycinGene expressionHeat shock proteinHeat stress
Type
Research Article
Information
Zygote , Volume 30 , Issue 6 , December 2022 , pp. 854 - 862
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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