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Influence of interleukin 1 beta and tumour necrosis factor alpha on the in vitro growth, maturation and mitochondrial distribution of bovine oocytes from small antral follicles

Published online by Cambridge University Press:  31 October 2018

FEO Lima ,
FTG Bezerra ,
GB Souza ,
MHT Matos ,
R van den Hurk  and
JRV Silva Open the ORCID record for JRV Silva [Opens in a new window]
FEO Lima
Affiliation:
Biotechnology Nucleus of Sobral, Federal University of Ceara, Sobral, CE, Brazil
FTG Bezerra
Affiliation:
Biotechnology Nucleus of Sobral, Federal University of Ceara, Sobral, CE, Brazil
GB Souza
Affiliation:
Biotechnology Nucleus of Sobral, Federal University of Ceara, Sobral, CE, Brazil
MHT Matos
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, UNIVASF, Petrolina, Pernambuco, Brazil
R van den Hurk
Affiliation:
Department of Pathobiology, Faculty of Veterinary 13 Medicine, Utrecht University, Utrecht, The Netherlands
JRV Silva*
Affiliation:
Biotechnology Nucleus of Sobral, Federal University of Ceara, Sobral, CE, Brazil
*
Author for correspondence: JRV Silva, Federal University of Ceara, Av. Comandante Maurocélio Rocha Ponte 100, CEP 62041–040, Sobral, CE, Brazil. Tel:/Fax: +55 88 36118000. E-mail: jrvsilva@ufc.br
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Summary

This study aimed to investigate the effects of IL1β and TNFα on growth and maturation of oocytes from small follicles (1–3 mm) during in vitro culture. To this end, cumulus–oocyte complexes (COCs) with diameters of ~110 µm were cultured in TCM-199 medium alone or supplemented with IL1β (10 ng/ml), TNFα (10 ng/ml) or both for 48 h. The oocytes were measured at the beginning and at the end of the culture period. COCs were cultured for 20 h in pre-maturation medium and then half of the COCs of each group was destined for in vitro maturation and the remaining COCs were used to evaluate meiotic progression, mitochondrial distribution and the expression of mRNAs for GDF-9, c-Mos, Cyclin-B1 and H1foo. The results showed that COCs cultured with TNFα alone or together with IL1β had higher diameters than those cultured in control medium alone or supplemented with IL1β. Control oocytes isolated from large antral follicles (>5 mm) had heterogeneous distribution of mitochondria. Oocytes isolated from small antral follicles, that had been grown in vitro in TCM-199 alone or supplemented with TNFα had similar heterogeneous mitochondrial distribution before in vitro maturation (IVM). After IVM, mitochondria were heterogeneously distribution when cultured in TCM-199. However, when cultured with TNFα and/or IL1β, mitochondria were homogeneously distributed. Presence of TNFα and/or IL1β in TCM-199 culture medium did not influence the expression of mRNAs for GDF-9, c-Mos, Cyclin-B1 and H1foo. In conclusion, TNFα and a mixture of TNFα and IL1β both stimulated the growth of bovine oocytes during their in vitro culture, but do not influence gene expression in grown oocytes.

Keywords

Antral follicleBovineCytokinesIL1βMaturationTNFα
Type
Research Article
Information
Zygote , Volume 26 , Issue 5 , October 2018 , pp. 381 - 387
Copyright
© Cambridge University Press 2018 

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