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Impact of insemination technique, semen quality and oocyte cryopreservation on pronuclear morphology of zygotes derived from sibling oocytes

Published online by Cambridge University Press:  08 June 2009

Alessia Nicoli ,
Francesco Capodanno ,
Barbara Valli ,
Roberta Di Girolamo ,
Maria Teresa Villani ,
Andrea Nucera ,
Riccardo Focarelli  and
Giovanni B. La Sala
Alessia Nicoli*
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, 42100 Reggio Emilia, Italy. Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Francesco Capodanno
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Barbara Valli
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Roberta Di Girolamo
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Maria Teresa Villani
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
Andrea Nucera
Affiliation:
Department of Health Sciences, Section of Medical Statistics and Epidemiology, University of Pavia, Italy.
Riccardo Focarelli
Affiliation:
Department of Evolutionary Biology, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.
Giovanni B. La Sala
Affiliation:
Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy.
*
All correspondence to: Alessia Nicoli. Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, 42100 Reggio Emilia, Italy. Tel: +39 0522296354. Fax: +39 522295909. e-mail: nicoli.alessia@asmn.re.it
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Summary

Pronuclear morphology seems to be an important predictive value of zygote development and integrity. In this study we want to evaluate the effect of insemination technique, male factor and oocyte cryopreservation on pronuclear morphology of zygotes derived from sibling oocytes in our Centre of Reproductive Medicine, Department of Obstetrics and Gynecology, Arcispedale S. Maria Nuova, Reggio Emilia, Italy. Subjects (n = 190) were submitted to IVF cycles with non-frozen and frozen sibling oocytes. Morphological evaluations were assessed using zygote pronuclear morphology (pronuclei, nucleoli and axis) in four groups: Group 1: 144 zygotes from 85 conventional IVF cycles with non-frozen oocytes; Group 2: 164 zygotes from 85 intracytoplasmic sperm injection (ICSI) cycles with Group 1 patients' sibling frozen oocytes; Group 3: 221 zygotes from 123 ICSI cycles with non-frozen oocytes; Group 4: 197 zygotes from 123 ICSI cycles with Group 3 patients' sibling frozen oocytes. No differences between Group 1 and Group 2 were seen. Group 3 was statistically different from Group 4 in relation to the nucleolar morphology. Oocyte cryopreservation procedure modified the nucleolar morphology of zygotes only in the presence of poor semen quality.

Keywords

CryopreservationICSIIVFPronucleiZygote
Type
Research Article
Information
Zygote , Volume 18 , Issue 1 , February 2010 , pp. 61 - 68
Copyright
Copyright © Cambridge University Press 2009

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References

Capmany, G., Tailor, A., Braude, P.R. & Bolton, V.N. (1996). The timing of pronuclear formation, DNA synthesis and cleavage in the human 1-cell embryo. Mol. Hum. Reprod. 2, 299–306.CrossRefGoogle ScholarPubMed
Chatzimeletiou, K., Morrison, E.E., Prapas, Y. & Handyside, A.H. (2008). Symposium: Genetic and epigenetic aspects of assisted reproduction. The centrosome and early embryogenesis: clinical insights. Reprod. Biomed. Online 16, 485–91.CrossRefGoogle Scholar
Chen, C. 1986. Pregnancy after human oocyte cryopreservation. Lancet 1 (8486), 884–6.CrossRefGoogle ScholarPubMed
Chian, R.C., Huang, J.Y., Tan, S.L., Lucena, E., Saa, A., Rojas, A., Ruvalcaba Castellón, L.A., GarcíaAmador, M.I. Amador, M.I. & Montoya Sarmiento, J.E. (2008). Obstetric and perinatal outcome in 200 infants conceived from vitrified oocytes. Reprod. Biomed. Online 16, 608–10.CrossRefGoogle ScholarPubMed
Clarke, H. (2003). Italy approves controversial legislation on fertility treatment. Lancet 362, 2076.Google Scholar
Fabbri, R., Porcu, E., Marsella, T., Rocchetta, G., Venturoli, S. & Flamigni, C. (2001). Human oocyte cryopreservation: new perspectives regarding oocyte survival. Hum. Reprod. 16, 411–6.CrossRefGoogle ScholarPubMed
Gianaroli, L., Magli, M.C., Ferraretti, A.P., Fortini, D. & Greco, N. (2003). Pronuclear morphology and chromosomal abnormalities as scoring criteria for embryo selection. Fertil. Steril. 80, 341–9.Google Scholar
Gianaroli, L., Magli, M.C., Ferraretti, A.P., Lappi, M., Borghi, E. & Ermini, B. (2007). Oocyte euploidy, pronuclear zygote morphology and embryo chromosomal complement. Hum. Reprod. 1, 241–9.Google Scholar
Gook, D. & Edgar, D.H. (2007). Human oocyte cryopreservation. Hum. Reprod. Update 13, 591–605.CrossRefGoogle ScholarPubMed
Greenacre, M.J. (1993). Correspondence Analysis in Practice. London: Academic Press.Google Scholar
Imai, A., Furui, T. & Yamamoto, A. (2008). Preservation of female fertility during cancer treatment. Reprod. Med. Biol. 7, 17–27.CrossRefGoogle ScholarPubMed
James, A.N., Hennessy, S., Reggio, B., Weimer, K., Larsen, F. & Cohen, J. (2006). The limited importance of pronuclear scoring of human zygotes. Hum. Reprod. 17, 1–6.Google Scholar
Jones, A., Van Blerkom, J., Davis, P. & Toledo, A.A. (2004). Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence. Hum. Reprod. 19, 1861–6.Google Scholar
Kruger, T.F., Acosta, A.A., Simmons, K.F., Swanson, R.J., Matta, J.F. & Oehninger, S. (1988). Predictive value of abnormal morphology in in vitro fertilization. Fertil. Steril. 49, 112–7.Google Scholar
La Sala, G.B., Nicoli, A., Villani, M.T., Pescarini, M., Gallinelli, A. & Blickstein, I. (2006). Outcome of 518 salvage oocyte-cryopreservation cycles performed as a routine procedure in an in vitro fertilization program. Fertil Steril. 86, 1423–7.CrossRefGoogle Scholar
Lebart, L. (1984). Multivariate Descriptive Statistical Analysis. New York: Wiley.Google Scholar
Nicoli, A., Valli, B., Di Girolamo, R., Di Tommaso, B., Gallinelli, A. & La Sala, G.B. (2007). Limited importance of pre-embryo pronuclear morphology (zygote score) in assisted reproduction outcome in the absence of embryo cryopreservation. Fertil. Steril. 88 (Suppl. 2), 1167–73.CrossRefGoogle ScholarPubMed
Oktay, K., Cil, A.P. & Bang, H. (2006). Efficiency of oocyte cryopreservation: a meta-analysis. Fertil. Steril. 86, 70–80.Google Scholar
Palermo, G., Joris, H., Devroey, P. & Van-Steirteghem, A.C. (1992). Pregnancies after intracytoplasmic injection of a single spermatozoon into oocytes. Lancet 340, 17–8.CrossRefGoogle Scholar
Payne, D., Flaherty, S.P., Barry, M.F. & Matthews, C.D. (1997). Preliminary observation on polar body extrusion and pronuclear in human oocytes using time-lapse videocinematography. Hum. Reprod. 12, 532–41.CrossRefGoogle Scholar
Sathananthan, H., Ratnasooirya, W.D., de Silva, A. & Randeniya, P. (2006). Rediscovering Boveri's centrosome in Ascaris (1888): its impact on human fertility and development. Reprod. Biomed. Online 12, 254–70.CrossRefGoogle ScholarPubMed
Schatten, H. (2008). The mammalian centrosome and its functional significance. Histochem. Cell. Biol. 129, 667–86.Google Scholar
Scott, L. (2003). Pronuclear scoring as a predictor of embryo development. Reprod. Biomed. Online 6, 201–14.CrossRefGoogle ScholarPubMed
Seli, E. & Sakkas, D. (2005). Spermatozoal nuclear determinants of reproductive outcome: implications for ART. Hum. Reprod. Update 11, 337–49.Google Scholar
Terada, Y. (2004). Human sperm centrosomal function during fertilization, a novel assessment for male sterility. Hum. Cell. 17, 181–6.CrossRefGoogle ScholarPubMed
Turone, F. (2004). Italy to pass new law on assisted reproduction. Brit. Med. J. 329, 9.CrossRefGoogle Scholar
Whittingham, D.G. (1977). Fertilization in vitro and development to term of unfertilized mouse oocytes previously stored at –196 degrees C. J. Reprod. Fertil. 49, 89–94.CrossRefGoogle ScholarPubMed
World Health Organization (1999). WHO Laboratory Manual for the Examination of Human Semen and Sperm–Cervical Mucus Interaction. Cambridge: Cambridge University Press.Google Scholar
Zeron, Y., Pearl, M., Borochov, A. & Arav, A. (1990). Kinetic and temporal factors influence chilling injury of germinal vesicle and mature bovine oocytes. Cryobiology 38, 35–42.Google Scholar