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Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Published online by Cambridge University Press:  19 August 2019

Thais Rose dos Santos Hamilton Open the ORCID record for Thais Rose dos Santos Hamilton [Opens in a new window] ,
Gabriela Esteves Duarte ,
José Antonio Visintin  and
Mayra Elena Ortiz D’Ávila Assumpção Open the ORCID record for Mayra Elena Ortiz D’Ávila Assumpção [Opens in a new window]
Thais Rose dos Santos Hamilton
Affiliation:
Animal Reproduction Department, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil
Gabriela Esteves Duarte
Affiliation:
Animal Reproduction Department, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil
José Antonio Visintin
Affiliation:
Animal Reproduction Department, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil
Mayra Elena Ortiz D’Ávila Assumpção*
Affiliation:
Animal Reproduction Department, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil
*
Address for correspondence: Mayra Elena Ortiz D’Ávila Assumpção. Animal Reproduction Department, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil. Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP. CEP 05508-270, Brazil. Tel: +55 11 30917916. E-mail: meoaa@usp.br
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Summary

Long-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.

Keywords

GPxGROxidative stressSODTesticular insulation
Type
Short Communication
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 432 - 435
Copyright
© Cambridge University Press 2019 

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