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Human oocyte cytometry and fertilisation rate after subzonal insemination

Published online by Cambridge University Press:  26 September 2008

Jean Philippe Wolf ,
Sylvie Bulwa ,
Daniel Rodrigues  and
Pierre Jouannet
Jean Philippe Wolf*
Affiliation:
Laboratoire de Biologie de la Reproduction, Histologie, Embryologie, Université Paris V, Hôpital Cochin, Paris, France.
Sylvie Bulwa
Affiliation:
Laboratoire de Biologie de la Reproduction, Histologie, Embryologie, Université Paris V, Hôpital Cochin, Paris, France.
Daniel Rodrigues
Affiliation:
Laboratoire de Biologie de la Reproduction, Histologie, Embryologie, Université Paris V, Hôpital Cochin, Paris, France.
Pierre Jouannet
Affiliation:
Laboratoire de Biologie de la Reproduction, Histologie, Embryologie, Université Paris V, Hôpital Cochin, Paris, France.
*
J.P. Wolf, Laboratoire de Biologie de la Reproduction, Histologie, Embryologie, Université Paris V, Hôpital Cochin, 123 Bd. Port Royal, F-75014 Paris, France. Telephone: (1)42.34.10.05. Fax: (1)42.34.16.89.
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Summary

The cytometry of 545 oocytes was evaluated during subzonal insemination (SUZI; 85 attempts), on day 0 (egg retrieval and SUZI), day 1 and day 2(embryo transfer). On day 0, the egg and oolemma diameters (mean ± SD) were 164.0 ± 19.6 μm and 114.2±16.8 μ5m respectively.The zona thickness was 17.8± 13.4 μm and correlated with the oolemma diameter(r = 0.24, p < 0.001). The fertilisation rate was significantly lower for the smaller oocytes (less than 108 μm diameter) compared with the larger oocytes (over 108μm) (9.8% vs 21.2% respectively; p < 0.05). These was little variation in oocyte diameter according to nuclear status. However, oocyte diameter increased significantly between day 0 and day 1 (p < 0.001) for both fertilised and unfertilised oocytes. Six different indications for SUZI were investigated in detail: three with non-specific (normal and subnormal sperm with in vitro fertilization failure, oligoasthenospermia) and three with specific sperm defects (flagellar dyskinesia, absence of outer dynein arms, antisperm antibodies). Oocytes from the non-specific defect groups had significantly smaller diameters than the others (p < 0.05). The mean fertilisation rate was related to the mean oolemma diameter for the groups with non-specific sperm defects and the group lacking dynein arms (LODA) (r = 0.91, p < 0.05). Eggs from the groups of patients with LODA and those with antisperm antibodies had thicker zona pellucida than others (p < 0.05). These findings suggest that in addition to nuclear criteria of maturity, the growth of oocytes is an important factor for fertilising ability. Insufficient development of the ooplasm may contribute to fertilisation failure, particularly when sperm with functional defects are used. In contrast, a thick zona pellucida may prevent sperm with specific anomalies such as LODA or antisperm antibodies from penetrating into the perivitelline space.

Keywords

CytometryHuman oocyteZona pellucida
Type
Article
Information
Zygote , Volume 3 , Issue 2 , May 1995 , pp. 101 - 109
Copyright
Copyright © Cambridge University Press 1995

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References

Abramczuk, J. & Sawicki, W. (1974). Variation in dry mass and volume of non-fertilized oocytes and blastomeres of 1–, 2– and 4–celled mouse embryos. J. Exp. Zool. 188, 25–34.CrossRefGoogle Scholar
Chan, P.J. (1987a). Morphometric analysis of the zona pellucida and ooplasm of squirrel monkey (Saimiri sciureus) eggs. Primates 28, 411–15.CrossRefGoogle Scholar
Chan, P.J. (1987b). Developmental potential of human oocytes according to zona thickness. J. In Vitro Fertil. Embryo Transfer 4, 237–41.CrossRefGoogle Scholar
Clark, H.J. & Masui, Y. (1987). Dose-dependent relationship between oocyte cytoplasmic volume and transformation of sperm nuclei to metaphase chromosomes. J. Cell Biol. 104, 831–40.CrossRefGoogle Scholar
Cohen, J., Leigh Inge, K., Suzman, M., Wiker, S.R. & Wright, G. (1989). Videocinematography of fresh and cryopreserved embryos: a retrospective analysis of embryonic morphology and implantation. Fertil. Steril. 51, 820–7.CrossRefGoogle ScholarPubMed
Feneux, D., Serres, C. and Jouannet, P. (1985). Sliding spermatozoa: a dyskinesia responsible for human infertility? Fertil. Steril. 441, 508–11.CrossRefGoogle Scholar
Goyanes, V.J., Ron-Corzo, A., Costa, E. & Maneiro, E. (1990). Morphometric categorisation of the human oocyte and early conceptus. Hum. Reprod. 5, 613–18.CrossRefGoogle ScholarPubMed
Jouannet, P., Escalier, D., Serres, C. & David, G. (1983). Motility of human sperm without outer dynein arms. J. Submicrosc.Cytol. 15, 67–71.Google ScholarPubMed
Plachot, M., Veiga, A., Montagur, J.,de Grouchy, J., Calderon, G. et al. . (1988). Are clinical and biological parameters correlated with chromosomal disorders in early life? A multicentric study. Hum. Reprod. 3, 627–35.CrossRefGoogle ScholarPubMed
Sorensen, R. & Wassarman, P. (1976). Relationship between growth and meiotic maturation of the mouse oocyte. Dev. Biol. 50, 531–6.CrossRefGoogle ScholarPubMed
Sternlicht, A.L. & Schultz, R.M. (1981). Biochemical studies of mammalian oogenesis: kinetics of accumulation of total and poly(A) containing RNA during growth of the mouse oocyte. J. Exp. Zool. 215, 191–200.CrossRefGoogle ScholarPubMed
Tsuji, K. & Nakano, R. (1985). Relationship between human oocyte maturation and different folUcular sizes. Biol. Reprod. 32, 413–17.CrossRefGoogle ScholarPubMed
Van Blerkom, J. (1989). The origin and detection of chromosomal abnormalities in meiotically mature human oocytes obtained from stimulated follicles after failed fertilisation in vitro. Prog. Clin. Biol. Res. 296, 299–310.Google Scholar
Van Blerkom, J. & Henry, G. (1992). Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum. Reprod. 7, 379–90.CrossRefGoogle ScholarPubMed
Veeck, L.L. (1986). Human oocytes at the time of follicular harvest. In Atlas of the Human Oocyte and the Early Conceptus, ed. Veeck, L.L., pp. 5–131. Baltimore: Williams & Wilkins.Google Scholar
Wassarman, P.M. (1990). Regulation of mammalian fertilisation by zona pellucida glycoproteins. J. Reprod. Fert. Suppl. 42, 79–87.Google ScholarPubMed
Wassarman, P. & Albertini, D.F. (1994). The mammalian ovum. In The Physiology of Reproduction, ed. Neill, J.D. & Knobil, E., pp. 79–122. New York: Raven Press.Google Scholar
Weibel, E.R. & Bolender, R. (1973). Stereological techniques for electron microscopic morphometry. In Principles and Techniques of Electron Microscopy, ed. Hayat, M., pp. 237–96. New York: Van Nostrand Reinhold.Google Scholar
WHO. (1993). Analyse du sperme humain et de I'interaction des spermatozoïdes avec le mucus cervical, 3rd edn, pp. 3–22. Paris: Les Editions INSERM.Google Scholar
Wolf, J.P., Ducot, B., Kunstmann, J.M., Frydman, R. & Jouannet, P. (1992). Influence of sperm parameters on outcome of subzonal insemination in the case of previous IVF failure. Hum. Reprod. 7, 1407–13.CrossRefGoogle ScholarPubMed
Wolf, J.P., Feneux, D., Escalier, D., Rodrigues, D., Frydman, R. & Jouannet, P. (1993). Pregnancy after subzonal insemination with spermatozoa lacking outer dynein arms. J. Reprod. Fert. 97, 487–92.CrossRefGoogle ScholarPubMed
Wolf, J.P., De Almeida, M., Ducot, B., Rodrigues, D. & Jouannet, P. (1995a). High levels of sperm-associated antibodies impair sperm–oolemma interaction after subzonal insemination. Fertil. Steril. 95, 584–90.CrossRefGoogle Scholar
Zuccotti, M., Yanagimachi, R. & Yanagimachi, H. (1991). The ability of hamster oolemma to fuse with spermatozoa: its acquisition during oogenesis and loss after fertilisation. Development 112, 143–52.CrossRefGoogle Scholar