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Genome organisation in the murine sperm nucleus

Published online by Cambridge University Press:  26 September 2008

Carol Jennings  and
Don Powell
Carol Jennings
Affiliation:
Cell Determination Laboratory, Departement of Development and Signalling, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK.
Don Powell*
Affiliation:
Cell Determination Laboratory, Departement of Development and Signalling, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK.
*
Don Powell, The Babraham Institute, Babraham, Cambridge CB2 4AT. Telephone: 01223 832312 Ext 337. Fax: 01223 836481.
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Summary

The organisation of DNA sequences in the murine sperm nucleus was studied using in situ hybridisation of biotinylated DNA probes. The efficiency of this reaction was assessed using a dispersed repetitive DNA probe. Telomeric DNA was distributed around the nucleus. Centromeric and ribosomal DNA sequences occupied restricted domains in the sperm nucleus. DNA sequences for a transgene and a cluster of homeogenes occupied different, and rather less defined, domains. Together these results imply that both repetitive and protein-coding sequences are arranged in the nucleus in an ordered fashion.

Keywords

Genome organisationIn situ hybridisationNucleusSperm
Type
Article
Information
Zygote , Volume 3 , Issue 2 , May 1995 , pp. 123 - 131
Copyright
Copyright © Cambridge University Press 1995

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