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Function of leukaemia inhibitory factor in spermatogenesis of a teleost fish, the medaka Oryzias latipes

Published online by Cambridge University Press:  16 October 2019

Ryuichi Satoh ,
Hisanori Bando ,
Noriyoshi Sakai ,
Tomoya Kotani Open the ORCID record for Tomoya Kotani [Opens in a new window]  and
Masakane Yamashita Open the ORCID record for Masakane Yamashita [Opens in a new window]
Ryuichi Satoh
Affiliation:
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
Hisanori Bando
Affiliation:
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Noriyoshi Sakai
Affiliation:
Genetic Strains Research Centre, National Institute of Genetics, Mishima 411-8540, Japan
Tomoya Kotani
Affiliation:
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
Masakane Yamashita*
Affiliation:
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
*
Address for correspondence: Masakane Yamashita. Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060–0810, Japan. Tel: +81 11 706 4454. Fax: +81 11 706 4456. E-mail: myama@sci.hokudai.ac.jp
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Summary

In response to gonadotropins and androgens, testicular cells produce various molecules that control proper proliferation and differentiation of spermatogenic cells through their paracrine and autocrine actions. However, molecules functioning downstream of the hormonal stimulation are poorly understood. Leukaemia inhibitory factor (Lif) is known to maintain the pluripotency of stem cells including embryonic stem cells and primordial germ cells at least in vitro, but its actual roles in vivo remain to be elucidated. To clarify the function of Lif in teleost (medaka) testes, we examined the effects of Lif on spermatogenesis in a newly established cell culture system using a cell line (named Mtp1) derived from medaka testicular somatic cells as feeder cells. We found that addition of baculovirus-produced recombinant medaka Lif to the culture medium or co-culture with Lif-overexpressing Mtp1 cells increased the number of spermatogonia. In situ hybridization and immunohistochemical analyses of the medaka testes showed that mRNAs and proteins of Lif are expressed in spermatogonia and the surrounding Sertoli cells, with higher expression levels in type A (undifferentiated) spermatogonia than in type B (differentiated) spermatogonia. Our findings suggest that Lif regulates spermatogonial cell proliferation in the medaka.

Keywords

Cell cultureLif expressionMitosisSpermatogenesisTeleost testis
Type
Research Article
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 423 - 431
Copyright
© Cambridge University Press 2019 

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