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Finding of bands of higher molecular weight than expected in three proteins in bovine preimplantation embryos

Published online by Cambridge University Press:  11 June 2019

Veronika Kinterova Open the ORCID record for Veronika Kinterova [Opens in a new window] ,
Veronika Petruskova ,
Jiri Kanka  and
Tereza Toralova
Veronika Kinterova*
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic
Veronika Petruskova
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic Faculty of Science, Charles University in Prague, Prague, Czech Republic
Jiri Kanka
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic
Tereza Toralova
Affiliation:
Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic
*
*Address for correspondence: Veronika Kinterova. Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v. v. i., Rumburska 89, 277 21 Libechov, Czech Republic. E-mail: kinterova@iapg.cas.cz
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Summary

We report here the existence of bands of higher molecular weight after western blot analysis in three proteins – Skp1, p27 and IκBα in bovine preimplantation embryos. This finding is specific to preimplantation embryos (from the 2-cell stage to the blastocyst stage) and not differentiated fibroblast cells in which these bands were of expected molecular weight. We suggest that these bands of higher molecular weight represent a complex of proteins that are characteristic of preimplantation embryos.

Keywords

IκBαMolecular weightp27Preimplantation embryoSkp1
Type
Short Communication
Information
Zygote , Volume 27 , Issue 3 , June 2019 , pp. 187 - 189
Copyright
© Cambridge University Press 2019 

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