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Expression pattern of STAT5A gene during early bovine embryogenesis

Published online by Cambridge University Press:  28 August 2013

Krzysztof Flisikowski ,
Marc Boulhaeve ,
Fabiola P. Lopes ,
Eckhard Wolf  and
Lech Zwierzchowski
Krzysztof Flisikowski*
Affiliation:
Technische Universität München, Liesel-Beckmann-Str. 1, 85354 Freising, Germany.
Marc Boulhaeve
Affiliation:
Ludwig-Maximilians-Universität München, Hackerstrasse 27, 85764 Oberschleissheim, Germany.
Fabiola P. Lopes
Affiliation:
Ludwig-Maximilians-Universität München, Hackerstrasse 27, 85764 Oberschleissheim, Germany.
Eckhard Wolf
Affiliation:
Ludwig-Maximilians-Universität München, Hackerstrasse 27, 85764 Oberschleissheim, Germany.
Lech Zwierzchowski
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05–552 Magdalenka, Poland.
*
All correspondence to: Krzysztof Flisikowski. Technische Universität München, Liesel-Beckmann-Str. 1, 85354 Freising, Germany. Tel. +49 8161 712036. Fax. +49 8161 712108. e-mail: flisikowski@wzw.tum.de
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Summary

Growth hormone (GH) plays an important role in early embryo development. It has been shown to activate multiple pathways, the most comprehensively studied being the STAT/JAK (Signal transducers and activators of transcription/Janus kinase) pathway. The objective of the present study was to investigate STAT5A gene expression during early bovine embryogenesis. Real-time polymerase chain reaction (RT-PCR) was used to measure the abundance of STAT5A transcripts. The mRNA was present at all stages of preimplantation bovine embryos investigated. The most abundant STAT5A expression occurred at the 2-cell stage. Expression was markedly reduced between the 4-cell and 8-cell stages, coinciding with the known time of embryo genome activation and loss of maternal mRNAs. This finding suggests that the embryonic STAT5A gene is primarily activated by maternal gene products.

Type
Short Communication
Information
Zygote , Volume 23 , Issue 2 , April 2015 , pp. 307 - 311
Copyright
Copyright © Cambridge University Press 2013 

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