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Embryo production by parthenogenetic activation and fertilization of in vitro matured oocytes from Cebus apella

Published online by Cambridge University Press:  10 January 2012

Julianne S. Lima ,
Danuza L. Leão ,
Rafael V. Sampaio ,
Adriel B. Brito ,
Regiane R. Santos ,
Moysés S. Miranda ,
Otávio M. Ohashi  and
Sheyla F. S. Domingues
Julianne S. Lima*
Affiliation:
Rua Augusto Corrêa, Campus Básico, CEP 66075–110, Belém, Pará, Brazil. Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil.
Danuza L. Leão
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil.
Rafael V. Sampaio
Affiliation:
Laboratory of In Vitro Fertilization, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil.
Adriel B. Brito
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil.
Regiane R. Santos
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil. Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Moysés S. Miranda
Affiliation:
Laboratory of In Vitro Fertilization, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil.
Otávio M. Ohashi
Affiliation:
Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil. Laboratory of In Vitro Fertilization, Instituto de Ciências Biológicas, Universidade Federal do Pará, Brazil.
Sheyla F. S. Domingues
Affiliation:
Laboratory of Wild Animal Biology and Medicine, Universidade Federal do Pará, Brazil. Animal Science Post-graduation Program, Universidade Federal do Pará, Brazil.
*
All correspondence to: Julianne S. Lima. Rua Augusto Corrêa, Campus Básico, CEP 66075–110, Belém, Pará, Brazil. Tel:/Fax: +55 81 32018011. e-mail: juliannesilvabio@gmail.com
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Summary

The efficiency of in vitro fertilization (IVF) depends on the viability of spermatozoa. For capuchin monkeys (Cebus apella), in vitro capacitation of spermatozoa is challenging because of their unique seminal coagulum. Motile spermatozoa can be obtained after liquefaction of the semen coagulum in coconut water-based solution. The objective of the present study was to establish an optimal in vitro maturation (IVM) protocol for capuchin monkeys and to observe the effect of follicle stimulating hormone (FSH) and luteinising hormone (LH) on IVF and parthenogenetic activation (PA) of oocytes collected from unstimulated females. We assessed spermatozoa quality after recovery from seminal coagulum using the solution ACP-118® as an extender. Oocytes were matured in vitro for 36 or 40 h and subjected to IVF or PA by applying ionomycin combined either with 6-dimethylaminopurine (6-DMAP) or roscovitine. In total, 87% of oocytes reached metaphase II (MII) after 40 IVM and 4-cell embryo production was obtained after IVF and parthenogenesis using ionomycin/6-DMAP. ACP-118® was used successfully to harvest viable spermatozoa from semen coagulum and in the preservation of spermatozoa, which were able to fertilize oocytes in vitro.

Keywords

ACP-118®Capuchin monkeysFertilizationMaturationParthenogenesis
Type
Research Article
Information
Zygote , Volume 21 , Issue 2 , May 2013 , pp. 162 - 166
Copyright
Copyright © Cambridge University Press 2012

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References

Dixson, A.F. & Anderson, M.J. (2002). Sexual selection, seminal coagulation and copulatory plug formation in primates. Folia. Primatol. 73, 63–9.CrossRefGoogle ScholarPubMed
Domingues, S.F.S., Caldas-Bussiere, M.C., Petretski, M.D.A., Ohashi, O.M., Lima, J.S., Santos, R.R., Cordeiro, M.S. & Castro, P.H.G. (2010). Effects of follicular phase and oocyte–cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella. Fertil. Steril. 93, 1662–7.CrossRefGoogle ScholarPubMed
Grupen, C.G., Gilchrist, R.B., Nayudu, P.L., Barry, M.F., Schulz, S.J., Ritter, L.J. & Armstrong, D.T. (2007). Effects of ovarian stimulation, with and without human chorionic gonadotrophin, on oocyte meiotic and developmental competence in the marmoset monkey (Callithrix jacchus). Theriogenology 68, 861–72.CrossRefGoogle ScholarPubMed
Marchall, V.S., Wilton, L.J. & Moore, H.D.M. (1998) Parthenogenetic activation of marmoset (Callithrix jacchus) oocytes and the development of marmoset parthenogenones in vitro and in vivo. Biol. Reprod. 59, 1491–7.CrossRefGoogle Scholar
Mitalipov, S.M., Nusser, K.D. & Wolf, D.P. (2001) Parthenogenetic activation of rhesus monkey oocytes and reconstructed embryos. Biol. Reprod. 65, 253–9.CrossRefGoogle ScholarPubMed
Morgan, P.M., Boatman, D.E. & Bavister, B.D. (1991). In vitro maturation of ovarian oocytes from unstimulated rhesus monkeys: assessment of cytoplasmic maturity by embryonic development after in vitro fertilization. Biol. Reprod. 45, 89–93.CrossRefGoogle ScholarPubMed
Oliveira, K.G., Miranda, S.A., Leão, D.L., Brito, A.B., Santos, R.R. & Domingues, S.F.S. (2011). Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES-TRIS. Anim. Reprod. Sci. 123, 75–80.CrossRefGoogle ScholarPubMed
Paffoni, A., Brevini, T.A.L., Gandolfi, F. & Ragni, G (2008). Parthenogenetic activation: biology and applications in the ART Laboratory. Placenta 29, 121–5.CrossRefGoogle Scholar
Parrish, J.J., Susko-Parrish, J.L., Winer, W.A. & First, N.L. (1988). Capacitation of bovine sperm by heparin. Biol. Reprod. 38, 1171–80.CrossRefGoogle ScholarPubMed
Schramm, R.D. & Bavister, B.D. (1999). A macaque model for studying mechanisms controlling oocyte development and maturation in human and non-human primates. Hum. Reprod. 14, 2544–55.CrossRefGoogle ScholarPubMed
Silva, M.A., Peixoto, G.C., Santos, E.A., Castelo, T.S., Oliveira, M.F. & Silva, A.R. (2011) Recovery and cryopreservation of epididymal sperm from agouti (Dasiprocta aguti) using powdered coconut water (ACP-109c) and Tris extenders. Theriogenology. 76, 1084–9.CrossRefGoogle ScholarPubMed
Wolf, D.P., Thormahlen, S., Ramsey, C., Yeoman, R.R., Fanton, J. & Mitalipov, S. (2004). Use of assisted reproductive technologies in the propagation of rhesus macaque offspring. Biol. Reprod. 71, 486–93.CrossRefGoogle ScholarPubMed
Yamasaki, J., Iwatani, C., Tsuchiya, H., Okahara, J., Sankai, T. & Torii, R. (2011). Vitrification and transfer of cynomolgus monkey (Macaca fascicularis) embryos fertilized by intracytoplasmic sperm injection. Theriogenology 76, 33–8.CrossRefGoogle ScholarPubMed
Zheng, P., Wang, H., Bavister, B.D. & Ji, W. (2001). Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development. Hum. Reprod. 16, 300–5.CrossRefGoogle ScholarPubMed
Zheng, P. (2007). Effects of in vitro maturation of monkey oocytes on their developmental capacity. Anim. Reprod. Sci. 98, 56–71.CrossRefGoogle ScholarPubMed