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Effects of IAA in combination with FSH on in vitro culture of ovine preantral follicles

Published online by Cambridge University Press:  09 July 2009

Sonia H.F. Costa ,
Regiane R. Santos ,
Davide Rondina ,
Evelyn R. Andrade ,
Otávio M. Ohashi ,
Ana Paula R. Rodrigues  and
José R. Figueiredo
Sonia H.F. Costa
Affiliation:
LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Regiane R. Santos*
Affiliation:
Faculty of Veterinary Medicine, Utrecht University, Department of Equine Sciences, Veterinary Pharmaceuticals, Pharmacology and Toxicology Division, Yalelaan 114, 3584 CM, Utrecht, The Netherlands.
Davide Rondina
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Evelyn R. Andrade
Affiliation:
State University of Londrina, Londrina, PR, Brazil.
Otávio M. Ohashi
Affiliation:
Federal University of Pará, Faculty of Biology, Belém, Pará, Brazil.
Ana Paula R. Rodrigues
Affiliation:
LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
José R. Figueiredo
Affiliation:
LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
*
All correspondence to: Regiane Rodrigues dos Santos. Faculty of Veterinary Medicine, Utrecht University, Department of Equine Sciences, Veterinary Pharmaceuticals, Pharmacology and Toxicology Division, Yalelaan 114, 3584 CM, Utrecht, The Netherlands. Tel: +31 30 253 1078. e-mail: R.Rodriguesdossantos@uu.nl
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Summary

Ovarian cortical fragments from five adult ewes were in vitro cultured for 1, 3 or 5 days in the presence of minimum essential medium either supplemented or not by follicle-stimulating hormone (FSH) (100 ng/ml) or indole-3-acetic acid (IAA) (10, 20, 40 or 100 ng/ml), alone or in combination. After in vitro culture, ovarian fragments were submitted to follicular isolation and viability test was performed using trypan blue. Addition of IAA (10 ng/ml) to a free-FSH medium resulted in the highest percentages of viable follicles, but was progressively deleterious in higher concentrations (20, 40 and 100 ng/ml) if in absence of FSH. Follicular development was observed only when FSH was added to an IAA-free medium. In conclusion, IAA at a concentration of 10 ng/ml increases follicular survival in vitro. However, at high concentrations (20, 40 or 100 ng/ml), this auxin may be deleterious to preantral follicles, the addition of FSH to the medium being necessary.

Keywords

AntioxidantFSHIVCPhytohormonePreantral follicles
Type
Research Article
Information
Zygote , Volume 18 , Issue 1 , February 2010 , pp. 89 - 92
Copyright
Copyright © Cambridge University Press 2009

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References

Adriaens, I., Cortvrindt, R. & Smitz, J. (2004). Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence. Hum. Reprod. 19, 398–408.CrossRefGoogle ScholarPubMed
Amorim, C.A., Lucci, C.M., Rodrigues, A.P., Carvalho, F.C., Figueiredo, J.R., Rondina, D., Cecchi, R., Giorgetti, A., Martini, A. & Gonçalves, P.B. (2000). Quantitative and qualitative analysis of the effectiveness of a mechanical method for the isolation of preantral follicles from ovine ovaries. Theriogenology 53, 1251–62.CrossRefGoogle ScholarPubMed
Andrade, E.R., Marcondes Seneda, M., Alfieri, A.A., de Oliveira, J.A., Frederico Rodrigues Loureiro Bracarense, A.P., Figueiredo, J.R. & Toniolli, R. (2005). Interactions of indole acetic acid with EGF and FSH in the culture of ovine preantral follicles. Theriogenology 64, 1104–13.CrossRefGoogle ScholarPubMed
Braw-Tal, R. & Yossefi, S. (1997). Studies in vivo and in vitro on the initiation of follicle growth in the bovine ovary. J. Reprod. Fertil. 109, 165–71.CrossRefGoogle ScholarPubMed
Candeias, L.P., Folkes, L.K., Porssa, M., Parrick, J. & Wardman, P. (1995). Enhancement of lipid peroxidation by indole-3-acetic acid and derivatives: substituent effects. Free Radical Res. 23, 403–18.CrossRefGoogle ScholarPubMed
Cano, A., Alcaraz, O. & Arnao, M.B. (2003). Free radical-scavenging activity of indolic compounds in aqueous and ethanolic media. Anal. Bioanal. Chem. 376, 33–7.CrossRefGoogle ScholarPubMed
Chandra, J., Samali, A. & Orrenius, S. (2000). Triggering and modulation of apoptosis by oxidative stress. Free Radic. Biol. Med. 29, 323–33.CrossRefGoogle ScholarPubMed
Eppig, J.J. & Schroeder, A.C. (1989). Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation and fertilization in vitro. Biol. Reprod. 41, 268–76.CrossRefGoogle ScholarPubMed
Ferreira, M.A., Brasil, A.F., Silva, J.R., Andrade, E.R., Rodrigues, A.P. & Figueiredo, J.R. (2001). Effects of storage time and temperature on atresia of goat ovarian preantral follicles held in M199 with or without indole-3-acetic acid supplementation. Theriogenology 55, 1607–17.CrossRefGoogle ScholarPubMed
Kim, D.S., Jeon, S.E. & Park, K.C. (2004). Oxidation of indole-3-acetic acid by horseradish peroxidase induces apoptosis in G361 human melanoma cells. Cell Signal 16, 81–8.CrossRefGoogle ScholarPubMed
Saha, S., Shimizu, M., Geshi, M. & Izaike, Y. (2000). In vitro culture of bovine preantral follicles. Anim. Reprod. Sci. 63, 27–39.CrossRefGoogle ScholarPubMed
Santos, R.R., Van Den Hurk, R., Rodrigues, A.P., Costa, S.H., Martins, F.S., Matos, M.H., Celestino, J.J. & Figueiredo, J.R. (2007). Effect of cryopreservation on viability, activation and growth of in situ and isolated ovine early-stage follicles. Anim. Reprod. Sci. 99, 53–64.CrossRefGoogle ScholarPubMed
Tsai-Turton, M. & Luderer, U. (2006). Opposing effects of glutathione depletion and follicle-stimulating hormone on reactive oxygen species and apoptosis in cultured preovulatory rat follicles. Endocrinology 147, 1224–36.CrossRefGoogle ScholarPubMed
Van Den Hurk, R. & Santos, R.R. (2009). Development of fresh and cryopreserved early-stage ovarian follicles, with special attention to ruminants. Anim. Reprod. 6, 72–95.Google Scholar
Zhang, X., Li, X.H., Ma, X., Wang, Z.H., Lu, S. & Guo, Y.L. (2006). Redox-induced apoptosis of human oocytes in resting follicles in vitro. J. Soc. Gynecol. Invertig. 13, 451–8.CrossRefGoogle ScholarPubMed