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Effects of follicular fluids on the growth of porcine preantral follicle and oocyte

Published online by Cambridge University Press:  01 August 2008

T. Metoki ,
H. Iwata ,
M. Itoh ,
M. Kasai ,
A. Takajyo ,
A. Suzuki ,
T. Kuwayama  and
Y. Monji
T. Metoki
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
H. Iwata*
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan. Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
M. Itoh
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
M. Kasai
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
A. Takajyo
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
A. Suzuki
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
T. Kuwayama
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
Y. Monji
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
*
All correspondence to H. Iwata, Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan. Tel: +81 46 270 6587. Fax: +81 46 270 6587. e-mail: h1iwata@nodai.ac.jp
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Summary

We examined the effect of supplementing the culture medium with follicular fluid (FF) on the growth of porcine preantral follicles and oocytes. Firstly, preantral follicles were retrieved from ovaries and then FF was collected from all antral follicles that were 2–7 mm in diameter (AFF), which included large follicles of 4–7 mm in diameter (LFF) and small follicles of 2–3 mm in diameter (SFF). When preantral follicles with a diameter of 250 μm were cultured in medium containing AFF, the growth of follicles and oocytes was greater than when follicles were cultured in medium containing fetal calf serum (FCS). When this growth-promoting effect in AFF was compared for LFF and SFF, the LFF were shown to be significantly more effective than SFF. This LFF effect was lost, however, when the concentration of LFF in the medium was decreased from 5% to 0.5% or when LFF were heat treated (60 °C for 30 min) or trypsin was added. In contrast, a decrease in SFF concentration from 5% to 0.5% and heat treatment of the SFF enhanced preantral follicle growth. Furthermore, proteins obtained from LFF that had molecular weights greater than 10 kDa (LFF > 10 kDa) had similar, but relatively reduced, growth-promoting properties. The remaining three LFF protein fractions (<10 kDa or <100 kDa or >100 kDa), however, did not have these growth-promoting properties. In conclusion, the supplementation of medium with LFF, rather than serum, enhanced preantral follicle and oocyte growth. Factors that enhanced follicle development in LFF and factors that suppressed follicle development in SFF were proteins and these LFF factors ranged in size from 10 kDa to over 100 kDa.

Keywords

DiameterFollicular fluidOocytePigPreantral follicle
Type
Research Article
Information
Zygote , Volume 16 , Issue 3 , August 2008 , pp. 239 - 247
Copyright
Copyright © Cambridge University Press 2008

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References

Algriany, O., Bevers, M., Schoevers, E., Colenbrander, B. & Dieleman, S. (2004). Follicle size-dependent effects of sow follicular fluid on in vitro cumulus expansion, nuclear maturation and blastocyst formation of sow cumulus oocytes complexes. Theriogenology 62, 1483–97.CrossRefGoogle ScholarPubMed
Albertini, D.F., Combelles, C.M., Benecchi, E. & Carabatsos, M.J. (2001). Cellular basis for paracrine regulation of ovarian follicle development. Reproduction 121, 647–53.CrossRefGoogle ScholarPubMed
Bolamba, D., Floyd, A.A., McGlone, J.J. & Lee, V.H. (2002). Epidermal growth factor enhances expression of connexin 43 protein in cultured porcine preantral follicles. Biol. Reprod. 67, 154–60.CrossRefGoogle ScholarPubMed
Diaz, F.J., O'Brien, M.J., Wigglesworth, K. & Eppig, J.J. (2006). The preantral granulosa cell to cumulus cell transition in the mouse ovary: development of competence to undergo expansion. Dev. Biol. 299, 91–104.CrossRefGoogle ScholarPubMed
Hirao, Y., Nagai, T., Kubo, M., Miyano, T., Miyake, M. & Kato, S. (1994). In vitro growth and maturation of pig oocytes. J. Reprod. Fertil. 100, 333–9.CrossRefGoogle ScholarPubMed
Ito, M., Iwata, H., Kitagawa, M., Kon, Y., Kuwayama, T. & Monji, Y. (2007). Effect of follicular fluid collected from various diameter follicles on the progression of nuclear maturation and developmental competence of pig oocytes. Anim. Reprod. Sci. in press.Google Scholar
Mao, J., Wu, G., Smith, M.F., McCauley, T.C., Cantley, T.C., Prather, R. S., Didion, B.A. & Day, B.N. (2002). Effects of culture medium, serum type and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol. Reprod. 67, 1197–203.CrossRefGoogle ScholarPubMed
Mao, J., Smith, M.F., Rucker, E.B., Wu, G.M., McCauley, T.C., Cantley, T.C., Prather, R.S., Didion, B.A. & Day, B.N. (2004). Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation and stimulation of granulosal cell proliferation and suppression of apoptosis in vitro. J. Anim. Sci. 82, 1967–75.CrossRefGoogle ScholarPubMed
Miyano, T. & Manabe, N. (2007). Oocyte growth and acquisition of meiotic competence. Soc. Reprod. Fertil. Suppl. 63, 531–8.Google ScholarPubMed
Naito, K., Fukuda, Y. & Toyoda, Y. (1988). Effects of porcine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gamete Res. 21, 289–95.CrossRefGoogle ScholarPubMed
Shuttleworth, G., Broughton, , Pipkin, F. & Hunter, M.G. (2002). In vitro development of pig preantral follicles cultured in a serum-free medium and the effect of angiotensin I-I. Reproduction 123, 807–18.CrossRefGoogle Scholar
Telfer, E.E., Binnie, J.P., McCaffery, F.H. & Campbell, B.K. (2000). In vitro development of oocytes from porcine and bovine primary follicles. Mol. Cell Endocrinol. 25, 117–23.CrossRefGoogle Scholar
Vatzias, G. & Hagen, D.R. (1999). Effects of porcine follicular fluid and oviduct-conditioned media on maturation and fertilization of porcine oocytes in vitro. Biol. Reprod. 60, 42–8.CrossRefGoogle ScholarPubMed
Wu, J. & Tian, Q. (2007). Role of follicle stimulating hormone and epidermal growth factor in the development of porcine preantral follicle in vitro. Zygote 15, 233–40.CrossRefGoogle ScholarPubMed
Wu, J., Emery, B.R. & Carrell, D.T. (2001). In vitro growth, maturation, fertilization and embryonic development of oocytes from porcine preantral follicles. Biol. Reprod. 64, 375–81.CrossRefGoogle ScholarPubMed
Yoon, K.W., Shin, T.Y., Park, J.I., Roh, S., Lim, J.M., Lee, B.C., Hwang, W.S. & Lee, E.S. (2000). Development of porcine oocytes from preovulatory follicles of different sizes after maturation in media supplemented with follicular fluids. Reprod. Fertil. Dev. 12, 133–9.CrossRefGoogle ScholarPubMed