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Effect of NANOG overexpression on porcine embryonic development and pluripotent embryonic stem cell formation in vitro

Published online by Cambridge University Press:  09 December 2021

Gerelchimeg Bou Open the ORCID record for Gerelchimeg Bou [Opens in a new window] ,
Shimeng Guo ,
Jia Guo ,
Zhuang Chai ,
Jianchao Zhao ,
Yan Li  and
Zhonghua Liu
Gerelchimeg Bou*
Affiliation:
College of Animal Science, Inner Mongolia Agricultural University, Hohhot010018, China
Shimeng Guo
Affiliation:
College of Life Science, Northeast Agricultural University, Harbin150030, China
Jia Guo
Affiliation:
College of Life Science, Northeast Agricultural University, Harbin150030, China
Zhuang Chai
Affiliation:
College of Life Science, Northeast Agricultural University, Harbin150030, China
Jianchao Zhao
Affiliation:
College of Life Science, Northeast Agricultural University, Harbin150030, China
Yan Li
Affiliation:
College of Life Science, Northeast Agricultural University, Harbin150030, China
Zhonghua Liu
Affiliation:
College of Life Science, Northeast Agricultural University, Harbin150030, China
*
Author for correspondence: Gerelchimeg Bou. College of Animal Science, Inner Mongolia Agricultural University, Hohhot010018, China. E-mail: gerelchimeg@imau.edu.cn
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Summary

The efficiency of establishing pig pluripotent embryonic stem cell clones from blastocysts is still low. The transcription factor Nanog plays an important role in maintaining the pluripotency of mouse and human embryonic stem cells. Adequate activation of Nanog has been reported to increase the efficiency of establishing mouse embryonic stem cells from 3.5 day embryos. In mouse, Nanog starts to be strongly expressed as early as the morula stage, whereas in porcine NANOG starts to be strongly expressed by the late blastocyst stage. Therefore, here we investigated both the effect of expressing NANOG on porcine embryos early from the morula stage and the efficiency of porcine pluripotent embryonic stem cell clone formation. Compared with intact porcine embryos, NANOG overexpression induced a lower blastocyst rate, and did not show any advantages for embryo development and pluripotent embryonic stem cell line formation. These results indicated that, although NANOG is important pluripotent factor, NANOG overexpression is unnecessary for the initial formation of porcine pluripotent embryonic stem cell clones in vitro.

Keywords

NanogOverexpressionPluripotent cellPorcine early development
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 324 - 329
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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