Hostname: page-component-7479d7b7d-rvbq7 Total loading time: 0 Render date: 2024-07-08T08:09:48.728Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of maturation medium on in vitro cleavage of canine oocytes fertilized with fresh and cooled homologous semen

Published online by Cambridge University Press:  01 February 2007

B. de Ávila Rodrigues ,
L. Carboneiro dos Santos  and
J.L. Rodrigues
B. de Ávila Rodrigues*
Affiliation:
Laboratory of Embryology and Biotechnics of Reproduction, Faculty of Veterinary Medicine, UFRGS; Cx Postal 15004; Telephone: +55 51 33166126; Fax: +55 51 33167305 91501–970Porto Alegre, RS, Brazil. Department of Animal Medicine, Faculty of Veterinary Medicine, UFRGS; Av. Bento Gonçalves 9090; 91501–970 Porto Alegre, RS, Brazil.
L. Carboneiro dos Santos
Affiliation:
Laboratory of Embryology and Biotechnics of Reproduction, Faculty of Veterinary Medicine, UFRGS; Cx Postal 15004; Telephone: +55 51 33166126; Fax: +55 51 33167305 91501–970Porto Alegre, RS, Brazil.
J.L. Rodrigues
Affiliation:
Laboratory of Embryology and Biotechnics of Reproduction, Faculty of Veterinary Medicine, UFRGS; Cx Postal 15004; Telephone: +55 51 33166126; Fax: +55 51 33167305 91501–970Porto Alegre, RS, Brazil.
*
All correspondence to: B. de Ávila Rodrigues, Laboratory of Embryology and Biotechnics of Reproduction, Faculty of Veterinary Medicine, UFRGS; Cx Postal 15004, 91501–970 Porto Alegre, RS, Brazil. Tel: +55 51 33166126. Fax: +55 51 33167305. e-mail: berenice@portoweb.com.br
Get access Rights & Permissions [Opens in a new window]

Summary

This study evaluated the effect of three maturation media on the development of in vitro-matured and in vitro-fertilized dog oocytes. In Experiment 1 (non-comparative experiment) canine cumulus–oocyte complexes (COCs) were matured in vitro in TCM199 supplemented with estrous cow serum (10%) + gonadotropins + steroid (treatment A), TCM199 + estrous cow serum (10%) (treatment B), or TCM199 + polyvinylpyrrolidone (PVP) (4%) (treatment C). All maturation media contained a final concentration of 1 μg/ml of human somatotropin (hST). Oocytes were fertilized with fresh ejaculated sperm and development was assessed by cleavage. The objective of Experiment 2 (comparative experiment) was to compare the rates of cleavage and developmental capacity of COCs matured in vitro in same medium as in Experiment 1, and fertilized either with fresh ejaculated or with cooled extended homologous spermatozoa. In Experiments 1 and 2, oocytes fertilized with fresh semen were in vitro-matured for 48 h, while in Experiment 2 COCs fertilized with cooled semen were matured in vitro for 72 h. The results of Experiments 1 and 2 demonstrated that cleavage was not influenced by the oocyte's maturation environment. The results of Experiment 1 showed that pronucleus formation + cleavage (day 7 after IVF) was similar among treatments A, B and C (p = 0.277). Also, in Experiment 2, pronucleus formation + cleavage (day 7 after IVF) was not different for oocytes fertilized in vitro either with fresh or cooled semen and maturated in media A (p = 0.190), B (p = 0.393) or C (p = 0.687). In both experiments, the numbers of embryos that developed to the 6–8-cell stage were higher for oocytes matured in medium A and fertilized with fresh semen, when compared with numbers of oocytes matured in media B and C. Embryo development to the 6–8-cell stage of oocytes fertilized either with fresh or cooled sperm was observed in treatments A and C in Experiment 2. Cumulus cell expansion was similar among treatments in Experiment 1. In Experiment 2, cumulus cell expansion among treatments A, B and C was similar after 48 h or 72 h of IVM. In both experiments, the greatest expansion category seen was for category 2 (outer cumulus cells slightly expanded). No correlation between cumulus expansion and cleavage were observed. Polyspermy rates in oocytes matured in medium A, and fertilized with fresh sperm were not significantly different from polyspermy rates observed using media B and C, in both experiments. Our findings indicate that treatments A, B and C are similarly effective for the cleavage of dog oocytes. Furthermore, it was demonstrated that canine oocytes matured in vitro could be fertilized by homologous cooled spermatozoa and progress to cleavage.

Keywords

CleavageDogOocyteSpermZygote
Type
Research Article
Information
Zygote , Volume 15 , Issue 1 , February 2007 , pp. 43 - 53
Copyright
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ball, G.D., Ax, R.L. & First, N.L. (1980). Mucopolysaccharide synthesis accompanies expansion of bovine cumulus–oocytes complexes in vitro. In Functional Correlates Of Hormone Receptors In Reproduction. (eds Mahash, V.B., Muldoon, T.G., Saxana, B.B. & Sadler, W.A.), p. 561. North Holland, New York: Elsevier.Google Scholar
Bolamba, D., Russ, K., Olson, M.A., Sandler, J.L. & Durrant, B.S. (2002). In vitro maturation of bitch oocytes from advanced preantral follicles in synthetic oviduct fluid medium: serum is not essential. Theriogenology 58, 1689–1703.CrossRefGoogle Scholar
Cha, K.Y., Do, B.R., Chi, H.J. et al. (1992). Viability of human follicular oocytes collected from unstimulated ovaries and matured and fertilized in vitro. Reprod. Fertil. Dev. 4, 695–701.CrossRefGoogle Scholar
Chang, H.C., Liu, H., Zhang, J., Grifo, J. & Krey, L.C. (2005). Developmental incompetency of denuded mouse oocytes undergoing maturation in vitro is ooplasmic in nature and is associated with aberrant Oct-4 expression. Human Reprod. 20, 1958–68.CrossRefGoogle ScholarPubMed
Chanson, A., Nocera, D., Senn, A., Grandi, P. & Germond, M. (2001). Developmental of a well-defined medium for the in vitro maturation of immature bovine cumulus–oocyte complexes. J. Assist. Reprod. Genet. 18, 97–105.CrossRefGoogle Scholar
Chen, L., Russel, P.T. & Larsen, W.J. (1994). Sequential effects of follicle-stimulating hormone and luteinizing hormone on mouse cumulus expansion in vitro. Biol. Reprod. 51, 290–5.CrossRefGoogle ScholarPubMed
Chian, R.C., Ao, A., Clarke, H.J., Tulandi, T. & Tan, S.L. (1999). Production of steroids from human cumulus cells treated with different concentrations of gonadotropins during culture in vitro. Fertil. Steril. 71, 61–6.CrossRefGoogle ScholarPubMed
Chian, R.C., Buckett, W.M. & Tan, S.L. (2003). In vitro maturation of human oocytes. Reprod. Biomed. Online 8, 148–66.CrossRefGoogle Scholar
Chian, R.C., Lim, J.H. & Tan, S.L. (2004). State of the art in in vitro oocyte maturation. Curr. Opin. Obstet. Gynecol. 16, 211–9.CrossRefGoogle ScholarPubMed
Cinone, M., Ghneim, A., Caira, M., Dell A'quila, M.E. & Minoia, P. (1992). Collection and maturation of oocytes in the bitch. Proceedings of the 12th International Congress on Animal Reproduction. 4, 1767–9.Google Scholar
Dumoulin, J.C.M., Meijers, C.J.J., Bras, M., Coonen, E., Geraedts, J.P.M. & Evers, J.L.H. (1999). Effect of oxygen concentration on human in vitro fertilization and embryo culture. Human Reprod. 14, 465–9.CrossRefGoogle Scholar
England, G.C.W., Verstegen, J.P. & Hewitt, D.A. (2001). Pregnancy following in vitro fertilization of canine oocytes. Vet. Rec. 148, 20–22.CrossRefGoogle ScholarPubMed
Eppig, J. (2003–2004). Maturation of the oocyte cumulus oocyte complex. The Jackson Laboratory Scientific Report pp. 106–110. In http://www.jax.org/shared_docs/tjlscirpt_current.pdfGoogle Scholar
Faveta, L.A., Robert, C., St John, E.J., Betts, D.H. & King, W.A. (2004). Mol. Human. Reprod. 10, 383–92.CrossRefGoogle Scholar
Fischer, B. & Bavister, B.D. (1993). Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J. Reprod. Fertil. 99, 673–9.CrossRefGoogle ScholarPubMed
Fujitani, Y., Kasai, K., Ohtani, S., Nishimura, K., Yamada, M. & Utsumi, K. (1997). Effect of oxygen concentration and free radicals on in vitro development of in vitro-produced bovine embryos. J. Anim. Sci. 75, 483–9.CrossRefGoogle ScholarPubMed
Gardner, D.K. & Lane, M. (2004). Culture of the mammalian preimplantation embryo. In A Laboratory Guide to the Mammalian Embryo, (Gardner, D.K., Lane, M. & Watson, A.J.) p. 46. Oxford.CrossRefGoogle Scholar
Gilchrist, R.B., Ritter, L.J. & Armstrong, D.T. (2004). Oocyte–somatic cell interactions during follicle development in mammals. Anim. Reprod. Sci. 82–3, 431–46.CrossRefGoogle Scholar
Glied, D.W., Rosenkrans Jr, C.F., Rorie, A., Munyon, A.L., Piersin, J.N., Miller, G.F. & Rakes, J.M. (1996). Effects of media, serum, oviductal cells, and hormones during maturation on bovine embryo development in vitro. J. Dairy Sci. 79, 536–42.CrossRefGoogle Scholar
Günzel-Apel, A-R. (1994). Fertilitätskontrolle und Samenübertragung beim Hund. p. 79. Hannover: Gustav Fischer Verlag Jena.Google Scholar
Hewitt, D.A. & England, G.C.W. (1999). Influence of gonadotrophin supplementation on the in vitro maturation of bitch oocytes. Vet. Rec. 144, 237–9.CrossRefGoogle ScholarPubMed
Karagenc, L., Sertkaya, Z., Ciray, N., Ulug, U. & Bahçeci, M. (2004). Impact of oxygen concentration on embryonic development of mouse zygotes. Reprod. Biomed. Online 9, 409–17.CrossRefGoogle ScholarPubMed
Kidson, A. (2004). In vitro embryo development in the pig–impact of oocyte maturation milieu on blastocyst morphology and viability. Dissertation. Utrecht University, p. 143.Google Scholar
Kito, S. & Bavister, B.D. (1996). Gonadotropins, serum, and aminoacids alter nuclear maturation, cumulus expansion, and oocyte morphology in hamster cumulus–oocyte complexes in vitro. Biol. Reprod. 56, 1281–9.CrossRefGoogle Scholar
Massip, A., Mermillod, P., Van Langendonckt, A., Touze, J.L. & Dessy, F. (1995). Survival and viability of fresh and frozen-thawed in vitro bovine blastocyst. Reprod. Nutr. Dev. 35, 3–10.CrossRefGoogle Scholar
McAvey, B.A., Wortzman, G.B., Williams, C.J. & Evans, J.P. (2002). Involvement of calcium signaling and the actin cytoskeleton in the membrane block to polyspermy in mouse oocytes. Biol. Reprod. 67, 1342–52.CrossRefGoogle Scholar
Metcalfe, S. (1999). Assisted Reproduction in the Bitch. Thesis. Monash University, Australia, p. 161.Google Scholar
Mies Filho, A. (1987). Tecnologia do sêmen – I.II – Diluição, conservação e transporte de sêmen. In Reprodução Dos Animais E Inseminação Artificial, pp. 419, 470, 479–80. Porto Alegre: Sulina.Google Scholar
Moor, R. & Day, Y. (2001). Maturation of pig oocytes in vivo and in vitro. Reproduction (Suppl.) 58, 91–104.Google ScholarPubMed
Neild, D., Chaves, G., Flores, M., Mora, N., Beconi, M. & Agüero, A. (1999). Hyposmotic test in equine spermatozoa. Theriogenology 51, 721–7.CrossRefGoogle Scholar
Nickson, D.A., Boyd, J., Eckersall, P.D., Ferguson, J.M., Harvey, M.J.A. & Renton, J.P. (1993). Molecular biological methods for monitoring oocyte maturation and in vitro fertilization in bitches. J. Reprod. Fertil. (Suppl.) 47, 231–40.Google ScholarPubMed
Otoi, T., Murakami, M., Fujii, M., Tanaka, M., Ooka, A., Une, S. & Suzuki, T. (2000). Development of canine oocytes matured and fertilised in vitro. Vet. Rec. 146, 52–3.CrossRefGoogle ScholarPubMed
Parrish, J.J., Susko-Parrish, J., Winner, M.A. & First, N.L. (1988). Capacitation of bovine sperm by heparin. Biol. Reprod. 38, 1171–80.CrossRefGoogle ScholarPubMed
Rodrigues, B.A. & Rodrigues, J.L. (2003). Meiotic response of in vitro matured canine oocytes under different proteins and heterologous hormone supplementation. Reprod. Dom. Anim. 38, 58–62.CrossRefGoogle ScholarPubMed
Rodrigues, B.A., dos Santos, L.C. & Rodrigues, J.L. (2004). Embryonic development of in vitro matured and in vitro fertilized dog oocytes. Mol. Reprod. Dev. 67, 215–23.CrossRefGoogle ScholarPubMed
Rota, A., Furzi, C., Panzani, F. & Camilo, F. (2004). Studies on motility and fertility of cooled stallion spermatozoa. Reprod. Dom. Anim. 39, 103–9.CrossRefGoogle ScholarPubMed
Saleh, R.A. & Agarwal, A. (2002). Oxidative stress and male infertility: from research bench to clinical practice. J. Androl. 23, 737–52.CrossRefGoogle ScholarPubMed
Songsasen, N., Yu, I. & Leibo, S.P. (2002). Nuclear maturation of canine oocytes cultured in protein-free media. Mol. Reprod. Dev. 62, 407–15.CrossRefGoogle ScholarPubMed
Songsasen, N. & Wildt, D.E. (2005). Size of the donor follicle, but not stage of reproductive cycle or seasonality, influences meiotic competence of selected domestic dog oocytes. Mol. Reprod. Dev. DOI 10.1002/mrd.20330.Google Scholar
Srsen, V., Kalous, J., Nagyova, E., Sutovsky, P., King, W.A. & Motlik, J. (1998). Effects of follicle-stimulating hormone, bovine somatotrophin and okadaic acid on cumulus expansion and nuclear maturation of blue fox (Alopex lagopus) oocytes in vitro. Zygote 6, 299–309.CrossRefGoogle ScholarPubMed
Tervitt, H.R., Whittingham, D.G. & Rowson, L.E. (1972). Successful culture in vitro of sheep and cattle ova. J. Reprod. Fertil. 30, 493–7.CrossRefGoogle Scholar
Villamediana, P., Ruttlant, J., López-Bejar, M.A., Vidal, F. & Paramio, M.T. (1999). Changes in sona pellucida surface after in vivo and in vitro maturation of carine oocytes. Reprod. Dom. Anim. 34, 417–21.CrossRefGoogle Scholar
Willingham, L.A. & Kraemer, D.C. (2001). Effects of progesterone supplementation on the resumption of meiosis of canine oocytes matured in vitro. In Annual Conference of the International Embryo Transfer Society. Proceedings. Omaha: IETS 55, 498.Google Scholar
Willingham-Rocky, L.A., Hanna, C.B.M., Westhusin, M.E. & Kraemer, D.C. (2002). Effects of porcine somatotropin and epidermal growth factor on the resumption of meiosis of canine oocytes matured in vitro. Theriogenology 57, 742.Google Scholar
Willingham-Rocky, L.A., Hinrichs, K., Westhusin, M.E. & Kraemer, D.C. (2003). Effects of stage of oestrus cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro. Reproduction 126, 501–8.CrossRefGoogle ScholarPubMed
Yamada, S., Shimazu, Y., Kawaji, H., Nakazawa, M., Naito, K. & Toyoda, Y. (1992). Maturation, fertilization, and development of dog oocytes in vitro. Biol. Reprod. 46, 853–8.CrossRefGoogle ScholarPubMed
Yamada, S., Shimazu, Y., Kawano, Y., Nakazawa, M., Naito, K. & Toyoda, Y. (1993). In vitro maturation and fertilization of preovulatory dog oocytes. J. Reprod. Fertil. (Suppl.) 47, 227–9.Google ScholarPubMed
Yokoo, M., Miyahayashi, Y., Nagamura, T., Kimura, N., Sasada, H. & Sato, E. (2002). Identification of hyaluronic acid-binding proteins and their expressions in porcine cumulus–oocyte complexes during in vitro maturation. Biol. Reprod. 67, 1165–71.CrossRefGoogle ScholarPubMed
Zheng, Y.S. & Sirard, M.A. (1992). The effect of sera, bovine serum albumin and follicular cells on in vitro maturation and fertilization of porcine oocytes. Theriogenology 37, 779–90.CrossRefGoogle ScholarPubMed