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Effect of embryonic cell cycle of nuclear donor embryos on the efficiency of nuclear transfer in Japanese black cattle

Published online by Cambridge University Press:  01 May 2007

M. Kishi ,
R. Takakura ,
Y. Nagao ,
K. Saeki  and
Y. Takahashi
M. Kishi
Affiliation:
Embryo Transplantation Laboratory, Snow Brand Milk Products Co., Ltd. Tomakomai, 059-1365, Japan. Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
R. Takakura
Affiliation:
Embryo Transplantation Laboratory, Snow Brand Milk Products Co., Ltd. Tomakomai, 059-1365, Japan.
Y. Nagao
Affiliation:
Embryo Transplantation Laboratory, Snow Brand Milk Products Co., Ltd. Tomakomai, 059-1365, Japan.
K. Saeki
Affiliation:
Embryo Transplantation Laboratory, Snow Brand Milk Products Co., Ltd. Tomakomai, 059-1365, Japan.
Y. Takahashi*
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
*
All correspondence to: Yoshiyuki Takahashi, Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan. Tel: +81 11 706 5231. Fax: +81 11 706 5232. e-mail: ytaka@vetmed.hokudai.ac.jp
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Summary

In the present study, the development in vitro and in vivo of nuclear transfer (NT) embryos reconstructed with embryonic cells (blastomeres) at the 32- to 63-cell (sixth cell cycle) and 64- to 127-cell (seventh cell cycle) stages was investigated to determine the optimum range of embryonic cell cycles for yielding the highest number of identical calves in Japanese black cattle. Rates of development to the blastocyst stage (overall efficiency) were higher in the sixth cell-cycle stage (45%) than in the seventh cell-cycle stage (12%). After the transfer of the blastocysts reconstructed with blastomeres of the sixth and seventh cell cycle-stage embryos to recipient heifers, there were no differences in the pregnancy (14/35: 40% versus 3/13: 23%, respectively) or calving rates (11/39: 28% versus 3/13: 23%, respectively). These results indicate that the highest number of identical calves would be obtained by using sixth cell cycle (32- to 63-cell)-stage embryos as nuclear donors.

Keywords

BlastomeresEmbryonic cell cycleJapanese black cattleNuclear donorsNuclear transfer
Type
Research Articles
Information
Zygote , Volume 15 , Issue 2 , May 2007 , pp. 165 - 171
Copyright
Copyright © Cambridge University Press 2007

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