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DNA methylation pattern in mouse oocytes and their in vitro fertilized early embryos: effect of oocyte vitrification

Published online by Cambridge University Press:  31 October 2012

Ying Liang ,
Xiang-Wei Fu ,
Jun-Jie Li ,
Dian-Shuai Yuan  and
Shi-En Zhu
Ying Liang
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Xiang-Wei Fu
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Jun-Jie Li
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Dian-Shuai Yuan
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
Shi-En Zhu*
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
*
All correspondence to: Shi-En Zhu. Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China. Tel: +86 10 62731767. e-mail: zhushien@cau.edu.cn
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Summary

This study was conducted to investigate the pattern of DNA methylation in vitrified–thawed mouse oocytes and their in vitro fertilized early embryos. Firstly, mouse oocytes at metaphase II (MII) stage of meiosis were allocated randomly into three groups: (1) untreated (control); (2) exposed to vitrification solution without being plunged into liquid nitrogen (toxicity); or (3) vitrified by open-pulled straw (OPS) method (vitrification). Oocytes from all three groups were fertilized subsequently in vitro. The level of DNA methylation in the MII oocytes and their early embryos was then examined by immunofluorescence using an anti-5-methylcytosine (anti-5-MeC) monoclonal antibody and fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG. Developmental rates to 2-cell embryos (62.28%) and blastocysts (43.68%) of the vitrified–thawed oocytes were lower (P < 0.01) than those of fresh oocytes (81.47%, 61.99%) and vitrification solution treated (79.20%, 60.04%) oocytes. DNA methylation (as reflected by 5-MeC fluorescence intensity) in the vitrification group was less (P < 0.01) for MII oocyte and 2- to 8-cell stages compared with that in the control and toxicity groups. Accordingly, a reduction in global genomic methylation due to vitrification of MII oocytes may result in compromised in vitro developmental potential in early mouse embryos.

Keywords

Early EmbryoDNA MethylationMouseOocyteOPS Vitrification
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 138 - 145
Copyright
Copyright © Cambridge University Press 2012 

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