Hostname: page-component-848d4c4894-mwx4w Total loading time: 0 Render date: 2024-06-26T07:30:23.443Z Has data issue: false hasContentIssue false
  • English
  • Français

Cytological studies on induced mitogynogenesis in Japanese flounder Paralichthys olivaceus (Temminck et Schlegel)

Published online by Cambridge University Press:  21 January 2016

Jilun Hou ,
Guixing Wang ,
Xiaoyan Zhang  and
Haijin Liu
Jilun Hou*
Affiliation:
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Guixing Wang
Affiliation:
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Xiaoyan Zhang
Affiliation:
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Haijin Liu
Affiliation:
Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, 100141, China.
*
All correspondence to: Jilun Hou. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China. Tel.: +86 335 5922354. Fax: +86 335 4260826. E-mail: jilunhou@hotmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

The effect of hydrostatic pressure treatment on the induction of mitogynogenesis in the eggs of Japanese flounder Paralichthys olivaceus (Temminck et Schlegel) by using heterospecific sperm were studied. Before treatment, the eggs were at metaphase of the first mitosis. The spindle was disassembled by the treatment and then resembled in its pretreatment position, and the chromosomes were rearranged, i.e., the first mitosis was not blocked. During the second mitotic cycle, only a monopolar spindle was assembled in each blastomere and the chromosomes doubled, but cell cleavage was blocked. In the third cycle, mitosis proceeded normally with a bipolar spindle in each blastomere. Flow cytometric analysis of ploidy demonstrated that mitogynogenetic larvae were all diploid. The ultraviolet light-irradiated sperm of the red sea bream (Pagrus major) was condensed, formed a dense chromatin body, and randomly entered one blastomere.

Keywords

CytologyHydrostatic pressureJapanese flounder Paralichthys olivaceusMitogynogenesisMonopolar spindle
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 700 - 706
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bertotto, D., Cepollaro, F., Libertini, A., Barbaro, A., Francescon, A., Belvedere, P., Barbaro, J. & Colombo, N. (2005). Production of clonal founders in the European sea bass, Dicentrarchus labrax L., by mitotic gynogenesis. Aquaculture 246, 115–24.Google Scholar
Chourrout, D. (1984). Pressure-induced retention of second polar body and suppression of first cleavage in rainbow trout: production of all-triploids, all-tetraploids, and heterozygous and homozygous diploid gynogenetics. Aquaculture 36, 111–26.Google Scholar
Hinchcliffe, E.H. & Sluder, G. (2001). “It takes two to tango”: understanding how centrosome duplication is regulated throughout the cell cycle. Genes Dev. 15, 1167–81.CrossRefGoogle ScholarPubMed
Hinchcliffe, E.H., Cassels, G.O., Rieder, C.L. & Sluder, G. (1998). The coordination of centrosome reproduction with nuclear events during the cell cycle in the sea urchin zygote. J. Cell Biol. 140, 1417–26.Google Scholar
Ihssen, P.E., McKay, L.R., McMillan, I. & Phillips, R.B. (1990). Ploidy manipulation and gynogenesis in fishes: cytogenetic and fisheries applications. Trans. Am. Fish Soc. 119, 698–717.2.3.CO;2>CrossRefGoogle Scholar
Kobayashi, T. (1997). Survival and cytological observations on early development of normal, hybrid, and gynogenetic embryos of amago salmon. Fisheries Sci. 63, 33–6.CrossRefGoogle Scholar
Komen, H. & Thorgaard, G.H. (2007). Androgenesis, gynogenesis and the production of clones in fishes: a review. Aquaculture 269, 150–73.CrossRefGoogle Scholar
Loncarek, J., Sluder, G. & Khodjakov, A. (2007). Centriole biogenesis: a tale of two pathways. Nat. Cell Biol. 9, 736–8.CrossRefGoogle ScholarPubMed
Mazia, D., Harris, P. & Bibring, T. (1960). The multiplicity of the mitotic centers and the time-course of their duplication and separation. J. Biophys. Biochem. Cytol. 7, 1–20.Google Scholar
Morelli, M. & Aquacop. (2003). Effects of heat-shock on cell division and microtubule organization in zygotes of the shrimp Penaeus indicus (Crustacea, Decapoda) observed with confocal microscopy. Aquaculture 216: 39–53.Google Scholar
Nam, Y.K., Choi, G.C., Park, D.J. & Kim, D.S. (2001). Survival and growth of induced tetraploid mud loach. Aquacult. Int. 9, 61–71.CrossRefGoogle Scholar
Sakao, S., Fujimoto, T., Tanaka, M., Yamaha, E. & Arai, K. (2003). Aberrant and arrested embryos from masu salmon eggs treated for tetraploidization by inhibition of the first cleavage. Nippon Suisan Gakk. 69, 738–48. [in Japanese]Google Scholar
Schartl, M., Wilde, B., Schlupp, I. & Parzefall, J. (1995). Evolutionary origin of a parthenoform, the Amazon molly Poecilia formosa, on the basis of a molecular genealogy. Evolution 49, 827–35.Google ScholarPubMed
Sluder, G. & Begg, D.A. (1985). Experimental analysis of the reproduction of spindle poles. J. Cell Sci. 76, 35–51.CrossRefGoogle ScholarPubMed
Streisinger, G., Walker, C., Dower, N., Knauber, D. & Singer, F. (1981). Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature 291, 293–6.CrossRefGoogle ScholarPubMed
Sun, W., You, F., Zhang, P.J. & Xu, J.H. (2005). Study on insemination biology of turbot (Psetta maxima) Mar. Sci. 29, 75–80. [in Chinese]Google Scholar
Yamamoto, E. (1995). Studies on sex-manipulation and production of cloned population in hirame flounder, Paralichthys olivaceus (Temminck et Schlegel). Bull. Tottori Pr. Fisheries Exp. Stat. 34, 1–145. [in Japanese]Google Scholar
Yamamoto, E. (1999). Studies on sex-manipulation and production of cloned population in hirame flounder, Paralichthys olivaceus (Temminck et Schlegel). Aquaculture 173, 235–46.Google Scholar
Yamashita, M., Jiang, J., Onozato, H., Nakanishi, T. & Nagahama, Y. (1993). A tripolar spindle formed at meiosis-I assures the retention of the original ploidy in the gynogenetic triploid crucian carp Ginbuna, Carassius auratus langsdorfii. Dev. Growth Differ. 35, 631–6.CrossRefGoogle ScholarPubMed
Zhang, X.L. & Onozato, H. (2004). Hydrostatic pressure treatment during the first mitosis does not suppress the first cleavage but the second one. Aquaculture 240, 101–13.CrossRefGoogle Scholar
Zhu, X.P., You, F., Zhang, P.J., Xu, Y.L. & Xu, J.H. (2006). Effects of cold shock on microtubule organization and cell cycle in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus). Mar. Biotechnol. 8, 312–8.Google Scholar
Zhu, X.P., You, F., Zhang, P.J., Xu, J.H. & Sun, W. (2007). Effects of hydrostatic pressure on microtubule organization and cell cycle in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus). Theriogenology 68, 873–81.CrossRefGoogle ScholarPubMed