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Comparison of two culture media on morphokinetics and ploidy status of sibling embryos

Published online by Cambridge University Press:  09 December 2021

Michael Urich ,
Muhammet Rasit Ugur ,
Fang Li ,
F. Nicholas Shamma ,
Ahmad Hammoud ,
Hanh N. Cottrell  and
Sule Dogan Open the ORCID record for Sule Dogan [Opens in a new window]
Michael Urich
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Muhammet Rasit Ugur
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Fang Li
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
F. Nicholas Shamma
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Ahmad Hammoud
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Hanh N. Cottrell
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
Sule Dogan*
Affiliation:
IVF Michigan, 37000 Woodward Ave, 350 Bloomfield Hills, Michigan 48304, USA
*
Author for correspondence: Sule Dogan. IVF Michigan, 37000 Woodward Ave #350 Bloomfield Hills, Michigan48304, USA. E-mail: suledogann@gmail.com
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Summary

To investigate the effects of culture media with different lactate concentrations on early embryonic development, data collected from our patients undergoing preimplantation genetic testing (PGT) were assessed using the EmbryoScope™ time-lapse culturing system. After intracytoplasmic sperm injection (ICSI), sibling oocytes were cultured in the same EmbryoScope (Vitrolife) slides including two different commercially available media. The patients with fewer than five mature oocytes were not included in the analyses. All embryos were hatched on day 3, and trophectoderm biopsies (n = 212) were performed accordingly. PGT for aneuploidy (PGT-A) on biopsied materials was carried out using next generation sequencing. Morphokinetic parameters, fertilization, irregular division, degeneration, blastulation, euploidy, and pregnancy rates of embryos cultured in LifeGlobal Global Total medium (LGGT) and Continuous Single Culture-NX Complete medium (CSCM-NXC) were compared. There were no differences observed in time to pronuclear fade, or in time spent as 2-cell (cc2) and 3-cell (s2), to 4-cell, 5-cell, morula and blastocyst stages (P > 0.05). Embryos reached the 2-cell (t2) and 3-cell (t3) stages significantly faster in LGGT (P < 0.05), whereas embryos grown in CSCM-NXC with lower lactate reached starting blastulation significantly sooner (P = 0.026). However, there were no statistical differences observed in fertilization, blastulation, degeneration, irregular division euploidy, and pregnancy rates between the two groups (P > 0.05). Even though pregnancy and fertilization rates did not indicate statistical differences, results are significant to provide better insight on potential roles of lactate in embryo development. These finding will advance the fundamental knowledge of human embryo development and assisted reproductive technologies.

Keywords

EuploidyLow lactate mediaMorphokineticsPreimplantation genetic testingTime-lapse system
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 410 - 415
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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References

Barberet, J, Chammas, J, Bruno, C, Valot, E, Vuillemin, C, Jonval, L, Choux, C, Sagot, P, Soudry, A and Fauque, P (2018). Randomized controlled trial comparing embryo culture in two incubator systems: G185 K-System versus Embryoscope. Fertil Steril 109, 302–9.e1.CrossRefGoogle ScholarPubMed
Biggers, JD, Whittingham, DG and Donahue, RP (1967). The pattern of energy metabolism in the mouse oocyte and zygote. Proc Natl Acad Sci USA 58, 560–7.CrossRefGoogle ScholarPubMed
Campbell, A, Fishel, S, Bowman, N, Duffy, S, Sedler, M and Hickman, CFL (2013). Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics. Reprod Biomed Online 26, 477–85.CrossRefGoogle ScholarPubMed
Cimadomo, D, Scarica, C, Maggiulli, R, Orlando, G, Soscia, D, Albricci, L, Romano, S, Sanges, F, Ubaldi, FM and Rienzi, L (2018). Continuous embryo culture elicits higher blastulation but similar cumulative delivery rates than sequential: A large prospective study. J Assist Reprod Genet 35, 1329–38.CrossRefGoogle ScholarPubMed
Costa-Borges, N, Bellés, M, Meseguer, M, Galliano, D, Ballesteros, A and Calderón, G (2016). Blastocyst development in single medium with or without renewal on day 3: A prospective cohort study on sibling donor oocytes in a time-lapse incubator. Fertil Steril 105, 707–13.CrossRefGoogle ScholarPubMed
Durand, M, Sermondade, N, Herbemont, C, Benard, J, Gronier, H, Boujenah, J, Cédrin-Durnerin, I, Poncelet, C, Grynberg, M and Sifer, C (2016). Embryo development in two single-step media: analysis of 2059 sibling oocytes. Gynecol Obstet Fertil 44, 163–7.CrossRefGoogle ScholarPubMed
Gardner, DK (2015). Lactate production by the mammalian blastocyst: manipulating the microenvironment for uterine implantation and invasion? BioEssays 37, 364–71.CrossRefGoogle ScholarPubMed
Gardner, DK, Schoolcraft, WB, Wagley, L, Schlenker, T, Stevens, J and Hesla, J (1998). A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. Hum Reprod 13, 3434–40.CrossRefGoogle ScholarPubMed
Gazzo, E, Peña, F, Valdéz, F, Chung, A, Bonomini, C, Ascenzo, M, Velit, M and Escudero, E (2020). The kidscoreⓞ d5 algorithm as an additional tool to morphological assessment and PGT-A in embryo selection: a time-lapse study. JBRA Assist Reprod 24, 55–60.Google ScholarPubMed
Gilbert, R, White, E and Ni, HT (2016). Low lactate culture benefits the in vitro development of preimplantation mammalian embryos. Abstract, 32nd Annual Meeting of the European Society of Human Reproduction and Embryology, Helsinki, Finland. Hum Reprod 31 (suppl 1), i235–6.Google Scholar
Khoury, C, Coffler, M, Potter, D, Frederick, J and Battaglia, D (2012). Improved blastocyst development using A single step medium versus A sequential medium. Fertil Steril 97, S4–5.CrossRefGoogle Scholar
Lane, M and Gardner, DK (2000). Lactate regulates pyruvate uptake and metabolism in the preimplantation mouse embryo. Biol Reprod 62, 16–22.CrossRefGoogle ScholarPubMed
Meseguer, M, Herrero, J, Tejera, A, Hilligsøe, KM, Ramsing, NB and Remohí, J (2011). The use of morphokinetics as a predictor of embryo implantation. Hum Reprod 26, 2658–71.CrossRefGoogle ScholarPubMed
Morbeck, DE, Baumann, NA and Oglesbee, D (2017). Composition of single-step media used for human embryo culture. Fertil Steril 107, 1055–60.e1.CrossRefGoogle ScholarPubMed
Patel, DV, Shah, PB, Kotdawala, AP, Herrero, J, Rubio, I and Banker, MR (2016). Morphokinetic behavior of euploid and aneuploid embryos analyzed by time-lapse in Embryoscope. J Hum Reprod Sci 9, 112–8.CrossRefGoogle ScholarPubMed
Quinn, P (2012). Culture systems: sequential. Method Mol Biol 912, 211–30.Google ScholarPubMed
Racowsky, C and Martins, WP (2017). Effectiveness and safety of time-lapse imaging for embryo culture and selection: It is still too early for any conclusions? Fertil Steril 108, 450–2.CrossRefGoogle ScholarPubMed
Racowsky, C, Kovacs, P and Martins, WP (2015). A critical appraisal of time-lapse imaging for embryo selection: where are we and where do we need to go? J Assist Reprod Genet 32, 1025–30.CrossRefGoogle ScholarPubMed
Rodríguez-Alonso, B, Maillo, V, Acuña, OS, López-Úbeda, R, Torrecillas, A, Simintiras, CA, Sturmey, R, Avilés, M, Lonergan, P and Rizos, D (2020). Spatial and pregnancy-related changes in the protein, amino acid, and carbohydrate composition of bovine oviduct fluid. Int J Mol Sci 21, 1681.CrossRefGoogle ScholarPubMed
Sciorio, R, Thong, JK and Pickering, SJ (2018). Comparison of the development of human embryos cultured in either an Embryoscope or benchtop incubator. J Assist Reprod Genet 35, 515–22.CrossRefGoogle ScholarPubMed
Sfontouris, IA, Kolibianakis, EM, Lainas, GT, Venetis, CA, Petsas, GK, Tarlatzis, BC and Lainas, TG (2017). Blastocyst utilization rates after continuous culture in two commercial single-step media: A prospective randomized study with sibling oocytes. J Assist Reprod Genet 34, 1377–83.CrossRefGoogle ScholarPubMed
Summers, MC, Bird, S, Mirzai, FM, Thornhill, A and Biggers, JD (2013). Human preimplantation embryo development in vitro: A morphological assessment of sibling zygotes cultured in a single medium or in sequential media. Hum Fertil 16, 278–85.CrossRefGoogle Scholar
Vera-Rodriguez, M, Chavez, SL, Rubio, C, Reijo Pera, RA and Simon, C (2015). Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis. Nat Commun 6, 7601.CrossRefGoogle ScholarPubMed
VerMilyea, M, Rios, C, Maizar, AMA, James, RM, Picou, A, Werland, HJ, Marrs, RP and Silverberg, K (2018). Stress relief: Can continuous culture in a low lactate culture media reduce numerical chromosomal abnormalities and therefore improve euploidy rates? American Society for Reproductive Medicine Scientific Congress Supplement Poster. Fertil Steril 110, e361–2.CrossRefGoogle Scholar
Werner, MD, Hong, KH, Franasiak, JM, Forman, EJ, Reda, CV, Molinaro, TA, Upham, KM and Scott, RT (2016). Sequential versus Monophasic Media Impact Trial (SuMMIT): A paired randomized controlled trial comparing a sequential media system to a monophasic medium. Fertil Steril 105, 1215–21.CrossRefGoogle ScholarPubMed
White, E, Popoff, T and Ni, HT (2017). Low lactate culture medium improves the development of human preimplantation embryos in vitro. Abstract, 33rd Annual Meeting of the European Society of Human Reproduction and Embryology, Geneva, Switzerland. Hum Reprod 32 (suppl 1), i229–30.Google Scholar