Hostname: page-component-7479d7b7d-jwnkl Total loading time: 0 Render date: 2024-07-12T01:16:56.906Z Has data issue: false hasContentIssue false

Comparative study between SpermSlow™ hyaluronan and traditional sperm selection in ICSI outcome

Published online by Cambridge University Press:  23 January 2023

Ahmed Abd Elraouf
Affiliation:
International Islamic Center for Population Studies and Research, Al-Azhar University, Darassa, Cairo, Egypt
Sayed Bakry
Affiliation:
Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
Ahmed Kadry
Affiliation:
International Islamic Center for Population Studies and Research, Al-Azhar University, Darassa, Cairo, Egypt
Othman F. Abdelzaher
Affiliation:
Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
Mohammed Adel*
Affiliation:
Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
*
Author for correspondence: Mohammed Adel. Laboratory of Embryology, Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt. E-mail: dr.m.adel2016@gmail.com

Summary

The role of hyaluronic acid (HA) as a ‘physiologic selector’ is also well recognized in vitro: it has been demonstrated that spermatozoa that bind to immobilized HA in vitro are those having completed their plasma membrane remodelling, and cytoplasmic and meiotic maturation. Sperm selection using HA has been expected to increase the implantation rate in intracytoplasmic sperm injection (ICSI) cycles. This work was designed to evaluate an alternative product for slowing sperm motility that contains HA and measures its outcomes: fertilization rate, embryo quality, and implantation and pregnancy rates. The present study found a positive drift in embryo quality that was statistically significant in the study group (SpermSlow™-ICSI) with teratozoospermia compared with PVP-ICSI in the same group. There were differences in the pregnancy rate (statistically insignificant in normozoospermia, asthenozoospermia, oligozoospermia, and teratozoospermia) in the SpermSlow-ICSI group compared with PVP-ICSI. The HA-ICSI technique in assisted reproduction technology (ART) is an important way to improve fertilization rate, embryo quality, and pregnancy rate.

Type
Research Article
Copyright
© The Author(s), 2023. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agarwal, A., Majzoub, A., Baskaran, S., Panner Selvam, M. K. P., Cho, C. L., Henkel, R., Finelli, R., Leisegang, K., SenGupta, P., Barbarosie, C., Parekh, N., Alves, M. G., Ko, E., Arafa, M., Tadros, N., Ramasamy, R., Kavoussi, P., Ambar, R., Kuchakulla, M., et al. (2020). Sperm DNA fragmentation: A new guideline for clinicians. World Journal of Men’s Health, 38(4), 412471. doi: 10.5534/wjmh.200128 CrossRefGoogle ScholarPubMed
Avalos-Durán, G., Cañedo-Del Ángel, A. M. E., Rivero-Murillo, J., Zambrano-Guerrero, J. E., Carballo-Mondragón, E. and Checa-Vizcaíno, M. Á. (2018). Physiological ICSI (PICSI) vs. conventional ICSI in couples with male factor: A systematic review. JBRA Assisted Reproduction, 22(2), 139147. doi: 10.5935/1518-0557.20180027 Google ScholarPubMed
Cohen, J. (2006). Micromanipulation as a clinical tool. In (ed. Gardner, D. K. in Vitro Fertilization. A Practical Approach. Citeseer, CRC Press.Google Scholar
Chan, Y. H. (2003a). Biostatistics102: Quantitative data – Parametric and non-parametric tests. Singapore Medical Journal, 44(8), 391396.Google Scholar
Chan, Y. H. (2003b). Biostatistics 103: Qualitative data – Tests of independence. Singapore Medical Journal, 44(10), 498503.Google ScholarPubMed
Duca, Y., Calogero, A. E., Cannarella, R., Condorelli, R. A. and La Vignera, S. (2019). Current and emerging medical therapeutic agents for idiopathic male infertility. Expert Opinion on Pharmacotherapy, 20(1), 5567. doi: 10.1080/14656566.2018.1543405 CrossRefGoogle ScholarPubMed
Erberelli, R. F., Salgado, R. M., Pereira, D. H. and Wolff, P. (2017). Hyaluronan-binding system for sperm selection enhances pregnancy rates in ICSI cycles associated with male factor infertility. JBRA Assisted Reproduction, 21(1), 26. doi: 10.5935/1518-0557.20170002 CrossRefGoogle ScholarPubMed
Esteves, S. C., Roque, M., Bedoschi, G., Haahr, T. and Humaidan, P. (2018) Intracytoplasmic sperm injection for male infertility and consequences for offspring. Nature Reviews. Urology, 15(9), 535562. doi: 10.1038/s41585-018-0051-8 CrossRefGoogle ScholarPubMed
Evans, E. P. P., Scholten, J. T. M., Mzyk, A., Reyes-San-Martin, C., Llumbet, A. E., Hamoh, T., Arts, E. G. J. M., Schirhagl, R. and Cantineau, A. E. P. (2021). Male subfertility and oxidative stress. Redox Biology, 46, 102071. doi: 10.1016/j.redox.2021.102071 CrossRefGoogle ScholarPubMed
Hill, G. A., Freeman, M., Bastias, M. C., Rogers, B. J., Herbert, C. M. III, Osteen, K. G. and Wentz, A. C. (1989). The influence of oocyte maturity and embryo quality on pregnancy rate in a program for in vitro fertilization-embryo transfer. Fertility and Sterility, 52(5), 801806. doi: 10.1016/s0015-0282(16)61034-8 CrossRefGoogle Scholar
Igawa, K., Yamashita, Y., Inoue, T., Taguchi, S., Tujimoto, Y., Kayoko, H., Yamamoto, S., Nakamura, N., Miyazaki, K., Terai, Y. and Ohmichi, M. (2019). The evaluation of sperm’s hyaluronic acid binding ability as a predictive value for the clinical success of intracytoplasmic sperm injection. Bulletin of the Osaka Medical College, 65, 1318.Google Scholar
Kim, S. J., Kim, H., Kim, T. H., Jeong, J., Lee, W. S. and Lyu, S. W. (2020). Effect of sperm selection using hyaluronan on fertilization and quality of cleavage-stage embryos in intracytoplasmic sperm injection (ICSI) cycles of couples with severe teratozoospermia. Gynecological Endocrinology, 36(5), 456459. doi: 10.1080/09513590.2019.1681960 CrossRefGoogle ScholarPubMed
Liu, Y., Feenan, K., Chapple, V., Roberts, P. and Matson, P. (2019). Intracytoplasmic sperm injection using hyaluronic acid or polyvinylpyrrolidone: A time-lapse sibling oocyte study. Human Fertility, 22(1), 3945. doi: 10.1080/14647273.2017.1366077 CrossRefGoogle ScholarPubMed
Longenecker, G., Cho, K., Khillan, J. S. and Kulkarni, A. B. (2021). Cryopreservation protocols for genetically engineered mice. Current Protocols, 1(5), e138. doi: 10.1002/cpz1.138 CrossRefGoogle ScholarPubMed
Miller, D., Pavitt, S., Sharma, V., Forbes, G., Hooper, R., Bhattacharya, S., Kirkman-Brown, J., Coomarasamy, A., Lewis, S., Cutting, R., Brison, D., Pacey, A., West, R., Brian, K., Griffin, D. and Khalaf, Y. (2019). Physiological, hyaluronan-selected intracytoplasmic sperm injection for infertility treatment (HABSelect): A parallel, two-group, randomised trial. Lancet, 393(10170), 416422. doi: 10.1016/S0140-6736(18)32989-1 CrossRefGoogle ScholarPubMed
Mishra, S., Jain, B. and Shankar, S. (2021). Comparison of physiological-ICSI (PICSI) with ICSI in cases of moderate to severe oligoasthenoteratozoospermia (OAT) − a randomized control study. Fertility Science and Research, 8(1), 4451. doi: 10.4103/fsr.fsr_40_20 Google Scholar
Morselli, M. (2016). Three-dimensional scaffolds for in vitro culture of feline oocytes.Google Scholar
Nabi, A., Entezari, F., Miresmaeili, S. Mohsen, Vahidi, S., Lorian, K., Anbari, F. and Motamedzadeh, L. (2021). Evaluation of sperm parameters and DNA integrity following different incubation times in PVP medium. Urology Journal, 19(3), 232237. doi: 10.22037/uj.v18i.6936 Google ScholarPubMed
Nel, M. (2015). A comparison of the effect of polyvinylpyrrolidone (PVP) and SpermSlow on human spermatozoa. Stellenbosch University.Google Scholar
Novoselsky Persky, M., Hershko-Klement, A., Solnica, A., Bdolah, Y., Hurwitz, A., Ketzin El Gilad, M., Nefesh, I. and Esh-Broder, E. (2021). Conventional ICSI vs. physiological selection of spermatozoa for ICSI (PICSI in sibling oocytes. Andrology, 9(3), 873877. doi: 10.1111/andr.12982 CrossRefGoogle ScholarPubMed
Oseguera-López, I., Ruiz-Díaz, S., Ramos-Ibeas, P. and Pérez-Cerezales, S. (2019). Novel techniques of sperm selection for improving IVF and ICSI outcomes. Frontiers in Cell and Developmental Biology, 7, 298. doi: 10.3389/fcell.2019.00298 CrossRefGoogle ScholarPubMed
Parmegiani, L., Cognigni, G. E., Bernardi, S., Troilo, E., Ciampaglia, W. and Filicori, M. (2010). ‘Physiological ICSI’: hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertility and Sterility, 93(2), 598604. doi: 10.1016/j.fertnstert.2009.03.033 CrossRefGoogle ScholarPubMed
Parmegiani, L., Cognigni, G. E. and Filicori, M. (2014). Sperm selection: Effect on sperm DNA quality. In Genetic damage in human spermatozoa (pp. 151–172). doi:10.1007/978-1-4614-7783-9_10CrossRefGoogle Scholar
Sciorio, R. and Smith, G. D. (2019). Embryo culture at a reduced oxygen concentration of 5%: A mini review; 27. Zygote, 27(6), 355361. doi: 10.1017/S0967199419000522 CrossRefGoogle Scholar
Wang, C., Feng, G., Shu, J., Zhou, H., Zhang, B., Chen, H., Lin, R., Gan, X., Wu, Z. and Wei, T. (2018). Cumulus oophorus complexes favor physiologic selection of spermatozoa for intracytoplasmic sperm injection. Fertility and Sterility, 109(5), 823831. doi: 10.1016/j.fertnstert.2017.12.026 CrossRefGoogle ScholarPubMed