Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-20T01:58:42.361Z Has data issue: false hasContentIssue false
  • English
  • Français

Combination of spindle and first polar body chromosome images for the enhanced prediction of developmental potency of mouse metaphase II oocytes

Published online by Cambridge University Press:  13 October 2016

Yukou Sugano ,
Manami Yazawa ,
Sachio Takino ,
Sueo Niimura  and
Hideaki Yamashiro
Yukou Sugano
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Manami Yazawa
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Sachio Takino
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Sueo Niimura
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
Hideaki Yamashiro*
Affiliation:
Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan
*
All correspondence to: Hideaki Yamashiro. Laboratory of Animal Reproduction, Graduate School of Science and Technology, Niigata University, 2–8050 Ikarashi, Nishiku, Niigata 950–2181, Japan. Tel:/Fax: +81 25 262 6596. E-mail: hyamashiro@agr.niigata-u.ac.jp
Get access Rights & Permissions [Opens in a new window]

Summary

The objective of this study was to classify spindle and first polar body (PB1) chromosome images in ovulated mouse oocytes over time to predict the developmental competence of metaphase II (MII) oocytes. Oocytes were collected at 12, 15, 20, and 25 h after human chorionic gonadotropin (hCG) injection, and stained for spindle tubulin, chromosomes, and PB1 chromosomes. MII spindle morphology was classified as tapered type or barrel type and PB1 chromosomes were categorized as aggregated, separated, dot, or collapsed. To determine whether differences in spindle and PB1 images in MII oocytes are associated with fertilization success, we performed in vitro fertilization (IVF) at various times after hCG injection. Barrel-type spindles and aggregate-type PB1 were dominant at 12 h after hCG injection. Oocyte spindles collected 1 h after injection were tapered, and PB1 chromosomes were separated. At 20 and 25 h after treatment, spindle and PB1 images were classified as collapsed. The rate of development to 2-cell embryos after IVF did not differ between the 12 h and 15 h treatments; however, it was significantly lower for the 25 h treatment than for other treatments. The rates of development to blastocysts at 12, 15, 20, and 25 h after hCG injection were 61, 46, 42, and 9%, respectively. MII oocytes with barrel-type spindles and aggregate-type PB1 had high rates of fertilization and blastocyst development, and spindle and PB1 characteristics were correlated with the outcomes of IVF and embryo culture. These results suggested that images of spindles combined with those of PB1 chromosomes enable the prediction of oocytic and/or embryonic quality.

Keywords

Developmental rateFirst polar bodyIVFMetaphase IISpindle
Type
Research Article
Information
Zygote , Volume 24 , Issue 6 , December 2016 , pp. 900 - 908
Copyright
Copyright © Cambridge University Press 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Battaglia, D.E., Goodwin, P., Klein, N.A. & Soules, M.R. (1996). Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum. Reprod. 11, 2217–22.Google Scholar
Capalbo, A., Bono, S., Spizzichino, L., Biricik, A., Baldi, M., Colamaria, S., Ubaldi, F.M., Rienzi, L. & Fiorentino, F. (2013). Sequential comprehensive chromosome analysis on polar bodies, blastomeres and trophoblast: insight into female meiotic errors and chromosomal segregation in the preimplantation window of embryo development. Hum. Reprod. 28, 509–18.Google Scholar
Christopikou, D., Tsorva, E., Economou, K., Shelley, P., Davies, S., Mastrominas, M. & Handyside, A.H. (2013). Polar body analysis by array comparative genomic hybridization accurately predicts aneuploidies of maternal meiotic origin in cleavage stage embryos of women of advanced maternal age. Hum. Reprod. 28, 1426–34.Google Scholar
Coticchio, G., Guglielmo, M.C., Dal Canto, M., Fadini, R., Mignini Renzini, M., De Ponti, E., Brambillasca, F. & Albertini, D.F. (2013). Mechanistic foundations of the metaphase II spindle of human oocytes matured in vivo and in vitro . Hum. Reprod. 28, 3271–82.Google Scholar
Fabian, D., Cikos, S., Rehak, P. & Koppel, J. (2012). Do embryonic polar bodies commit suicide? Zygote 22, 10–7.Google Scholar
Fabritius, A.S., Ellefson, M.L. & McNally, F.J. (2011). Nuclear and spindle positioning during oocyte meiosis. Curr. Opin. Cell. Biol. 23, 78–84.Google Scholar
Geraedts, J., Montag, M., Magli, M.C., Repping, S., Handyside, A., Staessen, C., Harper, J., Schmutzler, A., Collins, J., Goossens, V., van der Ven, H., Vesela, K. & Gianaroli, L. (2011). Polar body array CGH for prediction of the status of the corresponding oocyte. Part I: clinical results. Hum. Reprod. 26, 3173–80.Google Scholar
Igarashi, H., Takahashi, E., Hiroi, M. & Doi, K. (1997). Aging-related changes in calcium oscillations in fertilized mouse oocytes. Mol. Reprod. Dev. 48, 383–90.Google Scholar
Keefe, D., Kumar, M. & Kalmbach, K. (2015). Oocyte competency is the key to embryo potential. Fertil. Steril. 103, 317–22.CrossRefGoogle ScholarPubMed
Mailhes, J.B., Young, D. & London, S.N. (1998). Postovulatory ageing of mouse oocytes in vivo and premature centromere separation and aneuploidy. Biol. Reprod. 58, 1206–10.CrossRefGoogle ScholarPubMed
Montag, M., Van der Ven, K., Dorn, C. & Van der Ven, H. (2004). Outcome of laser-assisted polar body biopsy. Reprod. Biomed. Online 9, 425–9.Google Scholar
Montag, M., Koster, M., Strowitzki, T. & Toth, B. (2013). Polar body biopsy. Fertil. Steril. 100, 603–7.Google Scholar
Ortiz, M., Lucero, P. & Coxatto, H. (1983). Post ovulatory aging of human ova: spontaneous division of the first polar body. Gamete Res. 7, 269–76.Google Scholar
Sakai, C., Hoshino, Y., Sato, Y. & Sato, E. (2011). Evaluation of maturation competence of metaphase II oocytes in mice based on the distance between pericentriolar materials of meiotic spindle: distance of PCM during oocyte maturation. J. Assist. Reprod. Genet. 28, 157–66.CrossRefGoogle ScholarPubMed
Salvaggio, C.N., Forman, E.J., Garnsey, H.M., Treff, N.R. & Scott, R.T. Jr. (2014). Polar body based aneuploidy screening is poorly predictive of embryo ploidy and reproductive potential. J. Assist. Reprod. Genet. 31, 1221–6.Google Scholar
Sanfins, A., Lee, G.Y., Plancha, C.E., Overstrom, E.W. & Albertini, D.F. (2003). Distinctions in meiotic spindle structure and assembly during in vitro and in vivo maturation of mouse oocytes. Biol. Reprod. 69, 2059–67.CrossRefGoogle ScholarPubMed
Stern, H.J. (2014). Preimplantation genetic diagnosis: Prenatal testing for embryos finally achieving its potential. J. Clin. Med. 3, 280–309.Google Scholar
Uchiyama, K., Kikuchi, M., Ieda, S., Yamashita, N., Takehara, Y., Kaijima, H. & Kato, O. (2008). Efficiency of oocyte spindle observation with a LC-Polscope. J. Mamm. Ova Res. 25, 105–10.Google Scholar
Verlinsky, Y., Tur-Kaspa, I., Cieslak, J., Bernal, A., Morris, R., Taranissi, M., Kaplan, B. & Kuliev, A. (2005). Preimplantation testing for chromosomal disorders improves reproductive outcome of poor-prognosis patients. Reprod. Biomed. Online 11, 219–25.Google Scholar
Wei, Y., Zhang, T., Wang, Y.P., Schatten, H. & Sun, Q.Y. (2015). Polar bodies in assisted reproductive technology: current progress and future perspectives. Biol. Reprod. 92, 1–8.Google Scholar
Zhou, W., Fu, L., Sha, W., Chu, D. & Li, Y. (2015). Relationship of polar bodies morphology to embryo quality and pregnancy outcome. Zygote 22, 1–7.Google Scholar