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Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)

Published online by Cambridge University Press:  05 December 2018

Iván Valdebenito Isler Open the ORCID record for Iván Valdebenito Isler [Opens in a new window] ,
Elías Figueroa Villalobos ,
Leydy Sandoval ,
Pablo Contreras Mellado ,
Juan Carlos Sánchez Caamaño ,
Jorge Farías Avendaño  and
Jennie Risopatrón
Iván Valdebenito Isler*
Affiliation:
Temuco Catholic University, Faculty of Natural Resources, Temuco Chile; Alimentary Production Research Nucleus, School of Aquaculture; Universidad Catolica de Temuco, Acuicultura; Fundación Chile, Estación Experimental Quillaipe; Universidad de La Frontera, Ingeniería Química; and Universidad de La Frontera, CEBIOR
Elías Figueroa Villalobos
Affiliation:
Temuco Catholic University, Faculty of Natural Resources, Temuco Chile Alimentary Production Research Nucleus, School of Aquaculture
Leydy Sandoval
Affiliation:
Universidad Catolica de Temuco, Acuicultura
Pablo Contreras Mellado
Affiliation:
Temuco Catholic University, Faculty of Natural Resources, Temuco Chile Alimentary Production Research Nucleus, School of Aquaculture
Juan Carlos Sánchez Caamaño
Affiliation:
Fundación Chile, Estación Experimental Quillaipe
Jorge Farías Avendaño
Affiliation:
Universidad de La Frontera, Ingeniería Química
Jennie Risopatrón
Affiliation:
Universidad de La Frontera, CEBIOR, Chile
*
Author for correspondence: Iván Valdebenito Isler. Temuco Catholic University, Faculty of Natural Resources, Temuco Chile; or Alimentary Production Research Nucleus, School of Aquaculture, Chile. E-mail: ivisler@uct.cl
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Summary

There is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically. Of a ‘pool’ of incubated eggs at 10.7 ± 0.5°C, 100 microphotographs of blastodiscs were extracted at different incubation periods from 0.25 to 5 h after fertilization and analyzed. Blastodiscs taken at 3.5, 4.0 and 5.0 h were characterized and classified into symmetric or asymmetric groups according to their morphology. The proportions of length (L) and width (W) of each blastomere were determined to establish its symmetry. Additionally, 20 microphotographs of blastodiscs of normal appearance were analyzed morphologically (control blastodisc: CB) and compared other blastodiscs (4.0 and 5.0 h). The results showed that before fertilization oocytes presented a somehow turgid aspect (maximum average diameter of 987 ± 41 µm) and after fertilization and hydration, their diameter increased to 1001.5 ± 11 µm (but not statistically significant) and presented a spherical shape. First cleavage ends after 3.5 h of development, forming two blastomeres 467 ± 45 μm length (L) and 328 ± 21 μm width (W) with a L/W ratio of 1.43 ± 0.19. The second cleavage ends after development at 4.5 h forming four blastomeres 238 ± 65 μm length and 227 ± 65 μm width with a ratio L/W of 1.06 ± 0.09. Five categories were identified during the blastomere characterization: 70% normal or symmetric; 8% with odd numbers of blastomeres; 6% unequal; 6% ‘pie shaped’ and 10% amorphous.

Keywords

BlastomeresCleavageEleginops maclovinusNotothenidSegmentation
Type
Research Article
Information
Zygote , Volume 26 , Issue 6 , December 2018 , pp. 443 - 448
Copyright
© Cambridge University Press 2018 

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