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Artificial oocyte activation and human failed-matured oocyte vitrification followed by in vitro maturation

Published online by Cambridge University Press:  26 August 2011

Y. Liu ,
Y.X. Cao ,
Z.G. Zhang  and
Q. Xing
Y. Liu
Affiliation:
Department of Obstetrics and Gynecology, Maternal and Child Care Hospital of Hefei City, Hefei, China.
Y.X. Cao*
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China.
Z.G. Zhang
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, China.
Q. Xing
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, China.
*
All correspondence to: Yunxia Cao. Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022, China. Tel: +86 551 2922071. Fax: +86 551 2922071. E-mail: happysubmission@163.com
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Summary

The investigation presented in this paper was conducted on the effect of oocytes activation on frozen–thawed human immature oocytes followed by in vitro maturation (IVM). A total of 386 failed-matured oocytes (germinal vesicle (GV) and metaphase I (MI) stages) was randomly divided into two groups: fresh group and vitrification group, GV group and MI group, respectively). The matured oocytes were subject to intracytoplasmic sperm injection (ICSI) after IVM had been carried out. The vitrification group was randomly divided into two groups: controlled and artificial oocyte activation (AOA). The injected oocytes in the controlled group were cultured in cleavage medium. The AOA group oocytes were activated by exposing them to 7% anhydrous alcohol for 6 min then cultured in cleavage medium as well. The rates of fertilization and early embryonic development were compared between the controlled and AOA groups. In MI vitrification group, the high-quality embryo formation rate and blastocyst formation rate were significantly higher in the AOA group than in the controlled group (P < 0.01). In the GV vitrification group, the high-quality embryo formation rate was significantly higher in the AOA group than in the controlled group (P < 0.05). These results indicate that AOA may be good for early embryonic development of vitrified immature human oocytes.

Keywords

DevelopmentFertilizationHuman oocyteOocyte activationVitrification
Type
Research Article
Information
Zygote , Volume 21 , Issue 1 , February 2013 , pp. 71 - 76
Copyright
Copyright © Cambridge University Press 2011

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