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Antioxidant treatment during preservation of bovine ovaries increased the development potential of embryos

Published online by Cambridge University Press:  06 May 2010

Yoshikazu Nagao ,
Yumiko Harada ,
Mari Yamaguchi ,
Akane Igarashi ,
Yuki Ooshima  and
Yoku Kato
Yoshikazu Nagao*
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Shimokomoriya 443, Mohka, Tochigi 321–4415, Japan. University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan. Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan.
Yumiko Harada
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Mari Yamaguchi
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Akane Igarashi
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Yuki Ooshima
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321–4415, Japan.
Yoku Kato
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183–8509, Japan.
*
All correspondence to: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, Shimokomoriya 443, Mohka, Tochigi 321–4415, Japan. Tel/Fax: +81 285 84 1321. e-mail: ynagao@cc.utsunomiya-u.ac.jp
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Summary

The overnight preservation of bovine ovaries would be highly useful in the subsequent harvest of viable oocytes for reproductive study. The present study aimed to optimize conditions for overnight preservation of bovine ovaries by examining the effects of temperature, solution and supplementation. In Experiment 1, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved at 15°C was higher than that at either 5 or 25°C (p < 0.05). In Experiment 2, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved in University of Wisconsin solution was higher than when PBS or saline was used (p < 0.05). In Experiment 3, oocytes preserved in saline supplemented with 0.3 mM glutathione (GSH) exhibited an increase in the rate of blastocyst formation compared with oocytes supplemented with 0 or 3 mM GSH (p < 0.05). In Experiment 4, supplementation with 10 μM epigallocatechin gallate during ovary preservation increased the rate of blastocyst formation (p < 0.05). The blastocysts derived from ovaries stored in saline supplemented with GSH at 15°C for 24 h were shown to develop into normal offsprings following transfer to recipient heifers. Our studies indicate that bovine IVM/IVF embryos derived from ovaries preserved in saline supplemented with an antioxidant at 15°C for 24 h can successfully develop to the blastocyst stage and result in offspring.

Keywords

AntioxidantBovineOocyteOvaryPreservation
Type
Research Article
Information
Zygote , Volume 18 , Issue 4 , November 2010 , pp. 315 - 321
Copyright
Copyright © Cambridge University Press 2010

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References

Abe, S. & Shioya, Y. (1996). Effects of temperature and duration of preservation of bovine ovaries in physiological saline on the development of bovine embryos derived from follicular oocytes matured and fertilized in vitro. Anim. Sci. Technol. Jpn 67, 633–8.Google Scholar
Ali, A.A., Bilodeau, J.F. & Sirard, M.A. (2003). Antioxidant requirements for bovine oocytes vary during in vitro maturation, fertilization and development. Theriogenology 59, 939–49.CrossRefGoogle Scholar
Blondin, P., Coenen, K., Guilbault, L.A. & Sirard, M.A. (1997). In vitro production of bovine embryos: developmental competence is acquired before maturation. Theriogenology 47, 1061–75.CrossRefGoogle ScholarPubMed
Collins, G.M. & Wicomb, W.N. (1992). New organ preservation solutions. Kidney Int. Suppl. 38, S197–S202.Google ScholarPubMed
Delcorso, C.A., Cappiello, M. & Mura, U. (1994) Thiol dependent oxidation of enzymes: the last chance against oxidative stress. Int. J. Biochem. 26, 745–50.CrossRefGoogle Scholar
Flood, M.R., Gage, T.L. & Bunch, T.D. (1993). Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro. Theriogenology 39, 823–33.CrossRefGoogle ScholarPubMed
Guang, Z.W., Yu, S.D. & Xu, Z.R. (2007). Improvement in bovine embryo production in vitro by treatment with green tea polyphenols during in vitro maturation of oocytes. Anim. Reprod. Sci. 100, 22–31.Google Scholar
Guerin, P., Mouatassim, S. E.I. & Menezo, Y. (2001). Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7, 175–89.CrossRefGoogle ScholarPubMed
Guo, Q., Zhao, B.L., Li, M.F., Shen, S.R. & Xin, W.J. (1996). Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes. Biochim. Biophys. Acta 1304, 210–22.CrossRefGoogle ScholarPubMed
Iwata, H., Hayashi, T., Sato, H., Kimura, K., Kuwayama, T. & Monji, Y. (2005). Modification of ovary stock solution with magnesium and raffinose improves the developmental competence of oocytes after long preservation. Zygote 13, 303–8.CrossRefGoogle ScholarPubMed
Jaeschke, H., Smith, C.V. & Mitchell, J.R. (1988). Reactive oxygen species during ischemia-reflow injury in isolated perfused rat liver. J. Clin. Invest. 81, 1240–6.CrossRefGoogle ScholarPubMed
Jamieson, N.V., Sundberg, R., Lindell, S., Claesson, K., Moen, J., Vreugdenhil, P.K., Wight, D.G., Southard, J.H. & Belzer, F.O. (1988). Preservation of the canine liver for 24–48 hours using simple cold storage with UW solution. Transplantation. 46, 517–22.CrossRefGoogle ScholarPubMed
Koyama, I., Bulkley, G.B., Williams, G.M. & Im, M.J. (1985). The role of oxygen free radicals in mediating the reperfusion injury of cold-preserved ischemic kidneys. Transplantation 40, 590–5.CrossRefGoogle ScholarPubMed
Lafleur, M.V.M., Hoorweg, J.J., Joenje, H., Westmijze, E.J. & Retel, J. (1994). The ambivalent role of glutathione in the protection of DNA against single oxygen. Free. Radic. Res. 21, 9–17.CrossRefGoogle Scholar
Matos, D.G., Furnus, C.C., Moses, D.F. & Baldassarre, H. (1995). Effect of cysteamine on glutathione level and developmental capacity of bovine oocyte matured in vitro. Mol. Reprod. Dev. 42, 432–6.CrossRefGoogle ScholarPubMed
Matsushita, S., Tani, T., Kato, Y. & Tsunoda, Y. (2004). Effect of low-temperature bovine ovary storage on the maturation rate and developmental potential of follicular oocytes after in vitro fertilization, parthenogenetic activation, or somatic cell nucleus transfer. Anim. Reprod. Sci. 84, 293–301.CrossRefGoogle ScholarPubMed
Nagao, Y., Saeki, K., Hoshi, M., Takahashi, Y. & Kanagawa, H. (1995). Effects of water quality on in vitro fertilization and development of bovine oocytes in protein-free medium. Theriogenology 44, 433–44.CrossRefGoogle ScholarPubMed
Nagao, Y., Iijima, R. & Saeki, K. (2008). Interaction between embryos and culture conditions during in vitro development of bovine early embryos. Zygote 16, 127–33.CrossRefGoogle ScholarPubMed
Roberts, R.F., Nishanian, G. P., Carey, J.N., Sakamaki, Y., Starnes, V.A. & Barr, M.L. (1999). A comparison of the new preservation solution Celsior to Euro-Collins and University of Wisconsin solutions in lung reperfusion injury. Transplantation 67, 152–5.CrossRefGoogle ScholarPubMed
Saeki, K., Hoshi, M., Leibfried, M.L. & First, N.L. (1990). In vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormone. Theriogenology 34, 1035–9.CrossRefGoogle Scholar
Schroeder, P., Klotz, L.O. & Sies, H. (2003). Amphiphilic properties of (–)epicatechin and their significance of protection of cells against peroxynitrite. Biochem Biophys Res Commun. 307, 69–73.CrossRefGoogle ScholarPubMed
Vinson, J.A., Dabbagh, Y., Serry, M. & Jang, J. (1995). Plant flavonoids, especially tea flavonols, are powerful antioxidants using an in vitro oxidation model for heart disease. J. Agric. Food. Chem. 43, 2800–2.CrossRefGoogle Scholar
Vreugdenhil, P.K., Belzer, F.O. & Southard, J.H. (1991). Effect of cold storage on tissue and cellular glutathione. Cryobiology 28, 143–9.CrossRefGoogle ScholarPubMed
Yao, N., Wan, P.C., Hao, Z.D., Gao, F.F., Yang, L., Cui, M.S., Wu, Y., Liu, J.H., Liu, S., Chen, H. & Zeng, S.M. (2009). Expression of interferon-tau mRNA in bovine embryos derived from different procedures. Reprod Domest Anim. 44, 132–9.CrossRefGoogle ScholarPubMed
Yoshizawa, M., Konno, H., Zhu, S., Kageyama, S., Fukui, E., Muramatsu, S., Kim, S. & Araki, Y. (1999). Chromosomal diagnosis in each individual blastomere of 5- to 10-cell bovine embryos derived from in vitro fertilization. Theriogenology 51, 1239–50.CrossRefGoogle ScholarPubMed