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Aloe vera increases collagen fibres in extracellular matrix and mRNA expression of peroxiredoxin-6 in bovine ovarian cortical tissues cultured in vitro

Published online by Cambridge University Press:  01 December 2021

Francisco das Chagas Costa ,
Erlândia Márcia Vasconcelos ,
Venância Antônia Nunes Azevedo ,
Ernando Igo Teixeira de Assis ,
Laís Raiane Feitosa Melo Paulino ,
Anderson Weiny Barbalho Silva ,
José Roberto Viana Silva Open the ORCID record for José Roberto Viana Silva [Opens in a new window]  and
Ana Liza Paz Souza Batista
Francisco das Chagas Costa
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
Erlândia Márcia Vasconcelos
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
Venância Antônia Nunes Azevedo
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
Ernando Igo Teixeira de Assis
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
Laís Raiane Feitosa Melo Paulino
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
Anderson Weiny Barbalho Silva
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
José Roberto Viana Silva
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
Ana Liza Paz Souza Batista*
Affiliation:
Postgraduate Program in Biotechnology, Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
*
Author for correspondence: Ana Liza Paz Souza Batista. Federal University of Ceará, Av. Comandante Maurocélio Rocha Ponte, 100, CEP 62042–280, Sobral, CE, Brazil. Tel: /Fax: +55 88 36118000. E-mail: analizabatista@gmail.com
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Summary

In vitro culture of ovarian tissue containing primordial follicles is an important tool to study the initiation of follicular populations and to develop efficient culture systems to support in vitro follicle growth. Considering that in vitro culture favours oxidative stress, it is very important to supplement culture medium with antioxidant substances such as Aloe vera extract. This study aims to evaluate the effects of different concentrations of Aloe vera on the distribution of collagen fibres in the extracellular matrix, follicular activation, development and survival in bovine ovarian cortical tissues cultured in vitro, as well as on expression of mRNAs for antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), peroxiredoxin 6 (PRDX6) and glutathione peroxidase 1 (GPX1)]. To this end, ovarian cortical tissues were cultured for 6 days in α-MEM alone or supplemented with different concentrations of Aloe vera extract (1.0, 5.0, 10.0 or 50.0%). After culture, fragments were fixed and processed histologically to evaluate follicular morphology and activation, as well as the extracellular matrix by staining with picrosirius red. The levels of mRNA for SOD, CAT, PRDX6 and GPX1 in cultured ovarian tissues were evaluated by real-time polymerase chain reaction (PCR). Ovarian tissues cultured with 10.0 or 50.0% Aloe vera had higher percentages of collagen fibres than tissues cultured in control medium. A significant increase in developing follicles was observed in ovarian tissues cultured in α-MEM alone or supplemented with 10% Aloe vera when compared with fresh control or tissues cultured with 1.0% Aloe vera. Presence of Aloe vera did not influence the percentage of morphologically normal follicles when compared with control medium. Ovarian tissues cultured with 50.0% Aloe vera had higher percentages of morphologically normal follicles than those cultured with 10.0% Aloe vera. Furthermore, 10% Aloe vera significantly increased mRNA levels for PRDX6. In conclusion, 10.0% Aloe vera improves extracellular matrix distribution in cultured tissues and increases the expression of mRNA for PRDX6 after 6 days in vitro.

Keywords

Aloe veraAntioxidantBovine ovarian folliclesCollagenIn vitro culture
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 365 - 372
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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